Thioalbus denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thioalbus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3827 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369C7S2|A0A369C7S2_9GAMM Ankyrin repeat protein OS=Thioalbus denitrificans OX=547122 GN=DFQ59_10635 PE=4 SV=1
MM1 pKa = 6.95EE2 pKa = 4.9TSTPSKK8 pKa = 10.15NSRR11 pKa = 11.84AIWLASICLLVGVLGFGAVPASAASGIFTRR41 pKa = 11.84IFEE44 pKa = 4.35MEE46 pKa = 3.94GNAIDD51 pKa = 4.54EE52 pKa = 4.67NDD54 pKa = 3.16ASLYY58 pKa = 10.81KK59 pKa = 10.58EE60 pKa = 4.31GEE62 pKa = 3.73DD63 pKa = 3.24WQRR66 pKa = 11.84TNNDD70 pKa = 2.06IWVDD74 pKa = 3.2LWNTYY79 pKa = 10.06LPSATKK85 pKa = 8.66PTGWDD90 pKa = 2.89FDD92 pKa = 3.87VPYY95 pKa = 10.15SGKK98 pKa = 9.54AAVRR102 pKa = 11.84VFVPDD107 pKa = 3.59YY108 pKa = 9.91PGLPGFVGTIFDD120 pKa = 4.84DD121 pKa = 4.59NIFGAGCKK129 pKa = 9.84DD130 pKa = 3.69DD131 pKa = 6.19LDD133 pKa = 5.73LNTCKK138 pKa = 10.49SEE140 pKa = 4.22TGNSPDD146 pKa = 3.88KK147 pKa = 11.03NQWTNGYY154 pKa = 9.55AAFYY158 pKa = 10.19NIEE161 pKa = 4.05GLAAPVPVDD170 pKa = 3.44GLPPGTLYY178 pKa = 10.46HH179 pKa = 6.26QNGDD183 pKa = 3.66LVIVMGGDD191 pKa = 3.27VHH193 pKa = 8.54DD194 pKa = 5.38GDD196 pKa = 5.13GSSQVGGWLFRR207 pKa = 11.84QQVPAEE213 pKa = 4.33LIVPGEE219 pKa = 4.36DD220 pKa = 3.51FPFSRR225 pKa = 11.84SIGDD229 pKa = 3.38ILVTVDD235 pKa = 2.81WSEE238 pKa = 4.14GGRR241 pKa = 11.84TATVRR246 pKa = 11.84AYY248 pKa = 10.37RR249 pKa = 11.84WIGGDD254 pKa = 3.28TTEE257 pKa = 4.95CPTPGVVLGTDD268 pKa = 3.76DD269 pKa = 4.18NLCKK273 pKa = 10.27IFEE276 pKa = 4.66GPNAVCTPEE285 pKa = 3.91YY286 pKa = 10.8NGLACAIANYY296 pKa = 10.35APTEE300 pKa = 3.94EE301 pKa = 4.33APEE304 pKa = 4.49GVGEE308 pKa = 4.67TPAPWPYY315 pKa = 9.53IAKK318 pKa = 9.85GDD320 pKa = 3.98RR321 pKa = 11.84SVDD324 pKa = 3.38PANGIFTEE332 pKa = 4.34NFPEE336 pKa = 4.29TTLLEE341 pKa = 5.02GILNLSAILRR351 pKa = 11.84LNDD354 pKa = 3.21EE355 pKa = 4.61TVGCFSDD362 pKa = 5.35FMWEE366 pKa = 4.22TRR368 pKa = 11.84SSGAPGEE375 pKa = 4.36TYY377 pKa = 9.91NAKK380 pKa = 10.59LKK382 pKa = 10.7DD383 pKa = 3.84YY384 pKa = 11.0LLGDD388 pKa = 3.92TEE390 pKa = 4.64LCGIDD395 pKa = 3.48VDD397 pKa = 4.47KK398 pKa = 11.7SGGTLSKK405 pKa = 11.16VGDD408 pKa = 3.69LVSYY412 pKa = 10.71NILVEE417 pKa = 3.83NTGIVRR423 pKa = 11.84LYY425 pKa = 9.75KK426 pKa = 10.5QSITDD431 pKa = 3.62TLLGDD436 pKa = 4.04LTSTNGNCPGGDD448 pKa = 3.54EE449 pKa = 5.18DD450 pKa = 5.59NLTEE454 pKa = 4.65GEE456 pKa = 4.42SCVEE460 pKa = 4.24SNACGAYY467 pKa = 10.31LEE469 pKa = 4.87PDD471 pKa = 3.74GTCAITAWRR480 pKa = 11.84RR481 pKa = 11.84VATGDD486 pKa = 4.28PDD488 pKa = 4.13PLPNTVTVVYY498 pKa = 10.59DD499 pKa = 3.98SDD501 pKa = 4.15PGLQGDD507 pKa = 4.67EE508 pKa = 4.19VTSSNDD514 pKa = 2.82HH515 pKa = 6.09SVNLFQPAVEE525 pKa = 4.28VVKK528 pKa = 11.17GGDD531 pKa = 3.64TLSKK535 pKa = 10.98AGDD538 pKa = 3.59DD539 pKa = 3.58VTYY542 pKa = 11.16SFTINNLSSDD552 pKa = 4.07DD553 pKa = 4.21APNLLIDD560 pKa = 4.29DD561 pKa = 4.35TSGVTDD567 pKa = 3.62TVLGNLTATAKK578 pKa = 11.02ANGCTSLAYY587 pKa = 10.16GGSCSFNVVYY597 pKa = 9.18TVQSGDD603 pKa = 4.24PDD605 pKa = 4.13PLDD608 pKa = 3.44NTVSVLYY615 pKa = 10.71HH616 pKa = 6.9PDD618 pKa = 3.31GFPNNITDD626 pKa = 3.87EE627 pKa = 4.72DD628 pKa = 4.18SHH630 pKa = 8.01SVNLFQPSVDD640 pKa = 3.49VTKK643 pKa = 10.58TGDD646 pKa = 3.51TLSKK650 pKa = 11.03VGDD653 pKa = 3.77PVNYY657 pKa = 9.55TITVTNDD664 pKa = 2.78GSSDD668 pKa = 3.68NPGLEE673 pKa = 4.12NGTITDD679 pKa = 3.87TLLGNLLDD687 pKa = 4.24GSNPYY692 pKa = 8.2VTSSDD697 pKa = 3.59CTGTLGPSGSCTIQATRR714 pKa = 11.84TVQAGDD720 pKa = 4.24PDD722 pKa = 4.55PLPNTVTVHH731 pKa = 6.46FDD733 pKa = 3.25PTGSFDD739 pKa = 4.94NDD741 pKa = 2.95ITDD744 pKa = 3.76SDD746 pKa = 3.9GHH748 pKa = 5.8SVNLFQPGVDD758 pKa = 3.51VTKK761 pKa = 10.59TGDD764 pKa = 3.51TLSKK768 pKa = 10.94VGDD771 pKa = 3.69PASYY775 pKa = 9.97TITVNNTGSGDD786 pKa = 3.77SPNLVNGTISDD797 pKa = 3.99TLLGNLLDD805 pKa = 4.24GSNPYY810 pKa = 8.27VTSSTCSATLATGASCTIQATRR832 pKa = 11.84TVQAGDD838 pKa = 4.24PDD840 pKa = 4.55PLPNTVTVHH849 pKa = 5.52YY850 pKa = 10.62NPDD853 pKa = 3.4GFPNDD858 pKa = 3.34ISDD861 pKa = 4.19SDD863 pKa = 3.75GHH865 pKa = 5.89SVNLFQPGVDD875 pKa = 3.51VTKK878 pKa = 10.59TGDD881 pKa = 3.51TLSKK885 pKa = 10.94VGDD888 pKa = 3.69PASYY892 pKa = 9.97TITVNNTGSGDD903 pKa = 3.77SPNLVNGTISDD914 pKa = 3.99TLLGNLLDD922 pKa = 4.24GSNPYY927 pKa = 8.27VTSSTCSAALATGASCTIQATRR949 pKa = 11.84TVQAGDD955 pKa = 4.24PDD957 pKa = 4.55PLPNTVTVHH966 pKa = 5.52YY967 pKa = 10.62NPDD970 pKa = 3.4GFPNDD975 pKa = 3.82ISDD978 pKa = 3.8STT980 pKa = 3.85

Molecular weight: 102.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369CH38|A0A369CH38_9GAMM DinB family protein OS=Thioalbus denitrificans OX=547122 GN=DFQ59_102144 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.33NGRR28 pKa = 11.84QIIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3827

0

3827

1199143

29

2919

313.3

34.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.957 ± 0.055

0.98 ± 0.015

5.306 ± 0.031

6.628 ± 0.04

3.347 ± 0.022

9.124 ± 0.04

2.313 ± 0.021

4.147 ± 0.032

2.324 ± 0.034

11.576 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.194 ± 0.021

2.327 ± 0.021

5.507 ± 0.035

3.078 ± 0.027

8.375 ± 0.044

4.544 ± 0.025

4.83 ± 0.023

7.565 ± 0.032

1.42 ± 0.018

2.458 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski