Amino acid dipepetide frequency for Vibrio phage vB_VcaS_HC

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.425AlaAla: 8.425 ± 0.889
0.996AlaCys: 0.996 ± 0.182
5.105AlaAsp: 5.105 ± 0.473
6.89AlaGlu: 6.89 ± 0.692
2.905AlaPhe: 2.905 ± 0.363
4.648AlaGly: 4.648 ± 0.401
1.328AlaHis: 1.328 ± 0.245
5.894AlaIle: 5.894 ± 0.539
5.852AlaLys: 5.852 ± 0.613
8.591AlaLeu: 8.591 ± 0.634
2.739AlaMet: 2.739 ± 0.281
4.482AlaAsn: 4.482 ± 0.61
3.777AlaPro: 3.777 ± 0.333
4.648AlaGln: 4.648 ± 0.689
4.939AlaArg: 4.939 ± 0.457
4.731AlaSer: 4.731 ± 0.449
5.437AlaThr: 5.437 ± 0.584
5.23AlaVal: 5.23 ± 0.603
0.664AlaTrp: 0.664 ± 0.187
2.117AlaTyr: 2.117 ± 0.288
0.0AlaXaa: 0.0 ± 0.0
Cys
0.913CysAla: 0.913 ± 0.218
0.291CysCys: 0.291 ± 0.106
0.291CysAsp: 0.291 ± 0.12
0.872CysGlu: 0.872 ± 0.182
0.498CysPhe: 0.498 ± 0.129
1.038CysGly: 1.038 ± 0.226
0.291CysHis: 0.291 ± 0.105
0.581CysIle: 0.581 ± 0.15
1.038CysLys: 1.038 ± 0.198
1.121CysLeu: 1.121 ± 0.198
0.125CysMet: 0.125 ± 0.073
0.623CysAsn: 0.623 ± 0.173
0.706CysPro: 0.706 ± 0.178
0.457CysGln: 0.457 ± 0.124
0.83CysArg: 0.83 ± 0.17
0.54CysSer: 0.54 ± 0.163
0.789CysThr: 0.789 ± 0.162
0.706CysVal: 0.706 ± 0.164
0.125CysTrp: 0.125 ± 0.094
0.374CysTyr: 0.374 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
4.773AspAla: 4.773 ± 0.498
0.623AspCys: 0.623 ± 0.163
3.071AspAsp: 3.071 ± 0.331
3.984AspGlu: 3.984 ± 0.371
2.573AspPhe: 2.573 ± 0.336
2.864AspGly: 2.864 ± 0.332
1.37AspHis: 1.37 ± 0.238
2.698AspIle: 2.698 ± 0.33
3.943AspLys: 3.943 ± 0.486
7.18AspLeu: 7.18 ± 0.508
1.245AspMet: 1.245 ± 0.282
2.615AspAsn: 2.615 ± 0.287
3.901AspPro: 3.901 ± 0.483
3.279AspGln: 3.279 ± 0.41
3.901AspArg: 3.901 ± 0.338
2.656AspSer: 2.656 ± 0.372
3.03AspThr: 3.03 ± 0.362
3.196AspVal: 3.196 ± 0.393
0.747AspTrp: 0.747 ± 0.208
2.49AspTyr: 2.49 ± 0.338
0.0AspXaa: 0.0 ± 0.0
Glu
7.844GluAla: 7.844 ± 0.648
0.955GluCys: 0.955 ± 0.206
4.482GluAsp: 4.482 ± 0.469
4.482GluGlu: 4.482 ± 0.446
2.117GluPhe: 2.117 ± 0.279
4.69GluGly: 4.69 ± 0.512
1.743GluHis: 1.743 ± 0.303
4.69GluIle: 4.69 ± 0.435
3.984GluLys: 3.984 ± 0.486
7.927GluLeu: 7.927 ± 0.662
1.536GluMet: 1.536 ± 0.233
2.781GluAsn: 2.781 ± 0.367
1.785GluPro: 1.785 ± 0.283
4.067GluGln: 4.067 ± 0.663
3.652GluArg: 3.652 ± 0.429
4.026GluSer: 4.026 ± 0.448
5.23GluThr: 5.23 ± 0.384
5.188GluVal: 5.188 ± 0.468
0.623GluTrp: 0.623 ± 0.169
2.158GluTyr: 2.158 ± 0.286
0.0GluXaa: 0.0 ± 0.0
Phe
2.905PheAla: 2.905 ± 0.403
0.581PheCys: 0.581 ± 0.172
3.113PheAsp: 3.113 ± 0.404
3.03PheGlu: 3.03 ± 0.324
1.162PhePhe: 1.162 ± 0.232
2.615PheGly: 2.615 ± 0.326
0.457PheHis: 0.457 ± 0.134
2.241PheIle: 2.241 ± 0.327
2.449PheLys: 2.449 ± 0.349
3.03PheLeu: 3.03 ± 0.333
0.913PheMet: 0.913 ± 0.192
2.324PheAsn: 2.324 ± 0.376
0.872PhePro: 0.872 ± 0.237
1.204PheGln: 1.204 ± 0.218
1.619PheArg: 1.619 ± 0.254
2.158PheSer: 2.158 ± 0.278
2.075PheThr: 2.075 ± 0.311
2.075PheVal: 2.075 ± 0.271
0.0PheTrp: 0.0 ± 0.0
1.328PheTyr: 1.328 ± 0.242
0.0PheXaa: 0.0 ± 0.0
Gly
4.98GlyAla: 4.98 ± 0.469
0.664GlyCys: 0.664 ± 0.192
4.69GlyAsp: 4.69 ± 0.486
5.064GlyGlu: 5.064 ± 0.511
2.449GlyPhe: 2.449 ± 0.346
4.565GlyGly: 4.565 ± 0.485
0.996GlyHis: 0.996 ± 0.252
3.528GlyIle: 3.528 ± 0.411
3.237GlyLys: 3.237 ± 0.414
5.728GlyLeu: 5.728 ± 0.52
1.121GlyMet: 1.121 ± 0.241
2.905GlyAsn: 2.905 ± 0.41
0.0GlyPro: 0.0 ± 0.0
2.117GlyGln: 2.117 ± 0.311
3.154GlyArg: 3.154 ± 0.32
3.528GlySer: 3.528 ± 0.353
3.86GlyThr: 3.86 ± 0.481
4.482GlyVal: 4.482 ± 0.445
0.747GlyTrp: 0.747 ± 0.197
2.739GlyTyr: 2.739 ± 0.278
0.0GlyXaa: 0.0 ± 0.0
His
1.577HisAla: 1.577 ± 0.265
0.374HisCys: 0.374 ± 0.148
1.453HisAsp: 1.453 ± 0.288
1.37HisGlu: 1.37 ± 0.274
0.706HisPhe: 0.706 ± 0.182
1.204HisGly: 1.204 ± 0.236
0.498HisHis: 0.498 ± 0.136
0.706HisIle: 0.706 ± 0.18
1.577HisLys: 1.577 ± 0.267
1.743HisLeu: 1.743 ± 0.27
0.415HisMet: 0.415 ± 0.125
0.747HisAsn: 0.747 ± 0.19
0.996HisPro: 0.996 ± 0.232
0.789HisGln: 0.789 ± 0.143
1.453HisArg: 1.453 ± 0.257
1.079HisSer: 1.079 ± 0.222
1.245HisThr: 1.245 ± 0.244
1.287HisVal: 1.287 ± 0.22
0.125HisTrp: 0.125 ± 0.066
1.079HisTyr: 1.079 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.603IleAla: 5.603 ± 0.54
0.955IleCys: 0.955 ± 0.168
4.69IleAsp: 4.69 ± 0.481
5.894IleGlu: 5.894 ± 0.476
1.37IlePhe: 1.37 ± 0.234
3.154IleGly: 3.154 ± 0.472
1.204IleHis: 1.204 ± 0.262
2.532IleIle: 2.532 ± 0.386
3.943IleLys: 3.943 ± 0.509
3.86IleLeu: 3.86 ± 0.437
1.411IleMet: 1.411 ± 0.298
3.362IleAsn: 3.362 ± 0.372
2.905IlePro: 2.905 ± 0.338
2.158IleGln: 2.158 ± 0.37
3.694IleArg: 3.694 ± 0.508
3.652IleSer: 3.652 ± 0.406
3.362IleThr: 3.362 ± 0.375
3.154IleVal: 3.154 ± 0.38
0.332IleTrp: 0.332 ± 0.146
2.117IleTyr: 2.117 ± 0.366
0.0IleXaa: 0.0 ± 0.0
Lys
6.143LysAla: 6.143 ± 0.648
0.747LysCys: 0.747 ± 0.223
3.403LysAsp: 3.403 ± 0.448
4.15LysGlu: 4.15 ± 0.547
1.66LysPhe: 1.66 ± 0.346
2.864LysGly: 2.864 ± 0.363
1.785LysHis: 1.785 ± 0.312
2.822LysIle: 2.822 ± 0.424
3.943LysLys: 3.943 ± 0.601
5.852LysLeu: 5.852 ± 0.606
1.245LysMet: 1.245 ± 0.244
3.196LysAsn: 3.196 ± 0.456
4.067LysPro: 4.067 ± 0.531
3.652LysGln: 3.652 ± 0.517
3.86LysArg: 3.86 ± 0.5
2.988LysSer: 2.988 ± 0.459
3.652LysThr: 3.652 ± 0.534
2.698LysVal: 2.698 ± 0.406
0.623LysTrp: 0.623 ± 0.181
1.826LysTyr: 1.826 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
8.01LeuAla: 8.01 ± 0.806
1.162LeuCys: 1.162 ± 0.189
5.728LeuAsp: 5.728 ± 0.495
6.599LeuGlu: 6.599 ± 0.534
2.988LeuPhe: 2.988 ± 0.367
4.648LeuGly: 4.648 ± 0.44
1.826LeuHis: 1.826 ± 0.261
6.682LeuIle: 6.682 ± 0.576
5.686LeuLys: 5.686 ± 0.596
6.848LeuLeu: 6.848 ± 0.577
2.366LeuMet: 2.366 ± 0.27
6.06LeuAsn: 6.06 ± 0.546
3.652LeuPro: 3.652 ± 0.407
3.943LeuGln: 3.943 ± 0.432
4.897LeuArg: 4.897 ± 0.412
5.935LeuSer: 5.935 ± 0.524
6.558LeuThr: 6.558 ± 0.559
5.105LeuVal: 5.105 ± 0.498
0.623LeuTrp: 0.623 ± 0.184
2.573LeuTyr: 2.573 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
1.951MetAla: 1.951 ± 0.254
0.457MetCys: 0.457 ± 0.135
0.664MetAsp: 0.664 ± 0.146
0.498MetGlu: 0.498 ± 0.127
1.038MetPhe: 1.038 ± 0.24
1.038MetGly: 1.038 ± 0.21
0.498MetHis: 0.498 ± 0.125
1.826MetIle: 1.826 ± 0.256
1.577MetLys: 1.577 ± 0.241
1.992MetLeu: 1.992 ± 0.254
0.291MetMet: 0.291 ± 0.122
1.494MetAsn: 1.494 ± 0.25
1.162MetPro: 1.162 ± 0.204
0.955MetGln: 0.955 ± 0.205
1.328MetArg: 1.328 ± 0.191
0.913MetSer: 0.913 ± 0.19
1.619MetThr: 1.619 ± 0.301
1.577MetVal: 1.577 ± 0.301
0.208MetTrp: 0.208 ± 0.084
0.581MetTyr: 0.581 ± 0.17
0.0MetXaa: 0.0 ± 0.0
Asn
5.686AsnAla: 5.686 ± 0.521
0.54AsnCys: 0.54 ± 0.159
2.573AsnAsp: 2.573 ± 0.332
3.445AsnGlu: 3.445 ± 0.407
2.698AsnPhe: 2.698 ± 0.395
3.943AsnGly: 3.943 ± 0.429
1.038AsnHis: 1.038 ± 0.242
2.573AsnIle: 2.573 ± 0.387
2.739AsnLys: 2.739 ± 0.384
4.69AsnLeu: 4.69 ± 0.402
0.996AsnMet: 0.996 ± 0.173
2.822AsnAsn: 2.822 ± 0.355
2.49AsnPro: 2.49 ± 0.366
2.241AsnGln: 2.241 ± 0.326
2.573AsnArg: 2.573 ± 0.327
2.2AsnSer: 2.2 ± 0.293
3.279AsnThr: 3.279 ± 0.355
3.237AsnVal: 3.237 ± 0.433
0.457AsnTrp: 0.457 ± 0.116
1.743AsnTyr: 1.743 ± 0.251
0.0AsnXaa: 0.0 ± 0.0
Pro
2.034ProAla: 2.034 ± 0.301
0.581ProCys: 0.581 ± 0.139
2.49ProAsp: 2.49 ± 0.301
4.067ProGlu: 4.067 ± 0.466
1.245ProPhe: 1.245 ± 0.247
1.992ProGly: 1.992 ± 0.26
0.747ProHis: 0.747 ± 0.221
2.075ProIle: 2.075 ± 0.321
3.445ProLys: 3.445 ± 0.421
3.611ProLeu: 3.611 ± 0.384
0.374ProMet: 0.374 ± 0.136
1.992ProAsn: 1.992 ± 0.284
1.536ProPro: 1.536 ± 0.261
1.577ProGln: 1.577 ± 0.243
2.2ProArg: 2.2 ± 0.392
2.449ProSer: 2.449 ± 0.366
2.988ProThr: 2.988 ± 0.361
2.739ProVal: 2.739 ± 0.33
0.332ProTrp: 0.332 ± 0.14
1.204ProTyr: 1.204 ± 0.251
0.0ProXaa: 0.0 ± 0.0
Gln
4.275GlnAla: 4.275 ± 0.692
0.291GlnCys: 0.291 ± 0.113
1.743GlnAsp: 1.743 ± 0.256
3.071GlnGlu: 3.071 ± 0.422
1.743GlnPhe: 1.743 ± 0.239
3.196GlnGly: 3.196 ± 0.353
0.706GlnHis: 0.706 ± 0.162
3.237GlnIle: 3.237 ± 0.307
2.158GlnLys: 2.158 ± 0.366
4.897GlnLeu: 4.897 ± 0.53
0.83GlnMet: 0.83 ± 0.148
1.951GlnAsn: 1.951 ± 0.292
1.494GlnPro: 1.494 ± 0.254
2.034GlnGln: 2.034 ± 0.312
2.947GlnArg: 2.947 ± 0.621
2.905GlnSer: 2.905 ± 0.384
3.237GlnThr: 3.237 ± 0.384
2.781GlnVal: 2.781 ± 0.429
0.291GlnTrp: 0.291 ± 0.138
1.162GlnTyr: 1.162 ± 0.249
0.0GlnXaa: 0.0 ± 0.0
Arg
5.064ArgAla: 5.064 ± 0.55
0.332ArgCys: 0.332 ± 0.096
2.656ArgAsp: 2.656 ± 0.385
5.064ArgGlu: 5.064 ± 0.843
2.656ArgPhe: 2.656 ± 0.377
3.735ArgGly: 3.735 ± 0.424
1.162ArgHis: 1.162 ± 0.221
3.943ArgIle: 3.943 ± 0.386
3.528ArgLys: 3.528 ± 0.55
5.105ArgLeu: 5.105 ± 0.444
0.955ArgMet: 0.955 ± 0.198
2.449ArgAsn: 2.449 ± 0.3
1.868ArgPro: 1.868 ± 0.321
2.573ArgGln: 2.573 ± 0.467
3.777ArgArg: 3.777 ± 0.524
2.532ArgSer: 2.532 ± 0.322
3.32ArgThr: 3.32 ± 0.305
3.818ArgVal: 3.818 ± 0.393
0.415ArgTrp: 0.415 ± 0.139
1.619ArgTyr: 1.619 ± 0.201
0.0ArgXaa: 0.0 ± 0.0
Ser
5.479SerAla: 5.479 ± 0.587
0.54SerCys: 0.54 ± 0.181
3.362SerAsp: 3.362 ± 0.363
3.735SerGlu: 3.735 ± 0.356
2.075SerPhe: 2.075 ± 0.348
3.735SerGly: 3.735 ± 0.463
1.577SerHis: 1.577 ± 0.291
3.652SerIle: 3.652 ± 0.455
2.947SerLys: 2.947 ± 0.453
4.482SerLeu: 4.482 ± 0.456
1.204SerMet: 1.204 ± 0.249
3.196SerAsn: 3.196 ± 0.384
2.158SerPro: 2.158 ± 0.353
2.449SerGln: 2.449 ± 0.295
2.822SerArg: 2.822 ± 0.388
2.532SerSer: 2.532 ± 0.382
2.947SerThr: 2.947 ± 0.315
3.901SerVal: 3.901 ± 0.364
0.332SerTrp: 0.332 ± 0.111
1.785SerTyr: 1.785 ± 0.371
0.0SerXaa: 0.0 ± 0.0
Thr
5.313ThrAla: 5.313 ± 0.449
0.581ThrCys: 0.581 ± 0.134
3.735ThrAsp: 3.735 ± 0.373
4.109ThrGlu: 4.109 ± 0.429
2.615ThrPhe: 2.615 ± 0.366
5.23ThrGly: 5.23 ± 0.459
1.245ThrHis: 1.245 ± 0.258
3.777ThrIle: 3.777 ± 0.494
2.988ThrLys: 2.988 ± 0.385
6.35ThrLeu: 6.35 ± 0.566
1.536ThrMet: 1.536 ± 0.244
2.988ThrAsn: 2.988 ± 0.341
2.449ThrPro: 2.449 ± 0.335
2.573ThrGln: 2.573 ± 0.348
3.113ThrArg: 3.113 ± 0.363
3.445ThrSer: 3.445 ± 0.467
4.275ThrThr: 4.275 ± 0.484
4.399ThrVal: 4.399 ± 0.45
0.457ThrTrp: 0.457 ± 0.132
1.909ThrTyr: 1.909 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
5.105ValAla: 5.105 ± 0.441
0.913ValCys: 0.913 ± 0.195
4.067ValAsp: 4.067 ± 0.349
4.814ValGlu: 4.814 ± 0.512
2.283ValPhe: 2.283 ± 0.306
3.279ValGly: 3.279 ± 0.378
0.789ValHis: 0.789 ± 0.194
3.735ValIle: 3.735 ± 0.321
4.192ValLys: 4.192 ± 0.5
4.358ValLeu: 4.358 ± 0.441
1.287ValMet: 1.287 ± 0.245
3.445ValAsn: 3.445 ± 0.329
2.698ValPro: 2.698 ± 0.412
2.117ValGln: 2.117 ± 0.287
3.569ValArg: 3.569 ± 0.339
4.524ValSer: 4.524 ± 0.383
4.399ValThr: 4.399 ± 0.554
4.233ValVal: 4.233 ± 0.56
0.332ValTrp: 0.332 ± 0.12
2.034ValTyr: 2.034 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
0.374TrpAla: 0.374 ± 0.12
0.083TrpCys: 0.083 ± 0.062
0.415TrpAsp: 0.415 ± 0.142
0.415TrpGlu: 0.415 ± 0.128
0.125TrpPhe: 0.125 ± 0.073
0.54TrpGly: 0.54 ± 0.185
0.208TrpHis: 0.208 ± 0.101
0.664TrpIle: 0.664 ± 0.182
0.415TrpLys: 0.415 ± 0.133
1.121TrpLeu: 1.121 ± 0.201
0.208TrpMet: 0.208 ± 0.093
0.374TrpAsn: 0.374 ± 0.119
0.0TrpPro: 0.0 ± 0.0
0.623TrpGln: 0.623 ± 0.142
0.581TrpArg: 0.581 ± 0.147
0.457TrpSer: 0.457 ± 0.132
0.166TrpThr: 0.166 ± 0.086
0.706TrpVal: 0.706 ± 0.171
0.166TrpTrp: 0.166 ± 0.082
0.208TrpTyr: 0.208 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.947TyrAla: 2.947 ± 0.351
0.54TyrCys: 0.54 ± 0.172
2.407TyrAsp: 2.407 ± 0.353
2.449TyrGlu: 2.449 ± 0.338
1.245TyrPhe: 1.245 ± 0.213
1.577TyrGly: 1.577 ± 0.258
0.955TyrHis: 0.955 ± 0.219
1.494TyrIle: 1.494 ± 0.188
1.536TyrLys: 1.536 ± 0.28
3.403TyrLeu: 3.403 ± 0.366
0.83TyrMet: 0.83 ± 0.187
2.158TyrAsn: 2.158 ± 0.29
1.162TyrPro: 1.162 ± 0.258
1.494TyrGln: 1.494 ± 0.266
1.66TyrArg: 1.66 ± 0.24
1.66TyrSer: 1.66 ± 0.272
1.619TyrThr: 1.619 ± 0.264
1.66TyrVal: 1.66 ± 0.244
0.249TyrTrp: 0.249 ± 0.105
0.872TyrTyr: 0.872 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 113 proteins (24095 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski