Amino acid dipepetide frequency for Streptomyces phage VWB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.726AlaAla: 22.726 ± 1.523
1.173AlaCys: 1.173 ± 0.374
8.504AlaAsp: 8.504 ± 0.768
7.624AlaGlu: 7.624 ± 0.867
3.006AlaPhe: 3.006 ± 0.529
11.949AlaGly: 11.949 ± 1.23
2.346AlaHis: 2.346 ± 0.553
4.472AlaIle: 4.472 ± 0.792
3.812AlaLys: 3.812 ± 0.778
10.336AlaLeu: 10.336 ± 0.836
2.419AlaMet: 2.419 ± 0.392
1.833AlaAsn: 1.833 ± 0.407
8.724AlaPro: 8.724 ± 1.368
4.325AlaGln: 4.325 ± 0.515
13.049AlaArg: 13.049 ± 1.481
6.231AlaSer: 6.231 ± 0.637
7.697AlaThr: 7.697 ± 0.751
11.143AlaVal: 11.143 ± 1.079
2.712AlaTrp: 2.712 ± 0.408
2.273AlaTyr: 2.273 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
1.32CysAla: 1.32 ± 0.356
0.22CysCys: 0.22 ± 0.155
0.88CysAsp: 0.88 ± 0.273
0.586CysGlu: 0.586 ± 0.219
0.22CysPhe: 0.22 ± 0.136
0.88CysGly: 0.88 ± 0.25
0.293CysHis: 0.293 ± 0.154
0.147CysIle: 0.147 ± 0.106
0.293CysLys: 0.293 ± 0.153
0.586CysLeu: 0.586 ± 0.209
0.147CysMet: 0.147 ± 0.09
0.147CysAsn: 0.147 ± 0.119
0.88CysPro: 0.88 ± 0.286
0.147CysGln: 0.147 ± 0.099
1.1CysArg: 1.1 ± 0.28
0.953CysSer: 0.953 ± 0.414
0.586CysThr: 0.586 ± 0.209
0.513CysVal: 0.513 ± 0.204
0.44CysTrp: 0.44 ± 0.178
0.073CysTyr: 0.073 ± 0.053
0.0CysXaa: 0.0 ± 0.0
Asp
9.017AspAla: 9.017 ± 0.792
0.367AspCys: 0.367 ± 0.148
5.571AspAsp: 5.571 ± 1.808
3.299AspGlu: 3.299 ± 0.617
1.1AspPhe: 1.1 ± 0.285
6.891AspGly: 6.891 ± 0.82
1.466AspHis: 1.466 ± 0.3
2.126AspIle: 2.126 ± 0.343
1.393AspLys: 1.393 ± 0.379
5.278AspLeu: 5.278 ± 0.603
0.953AspMet: 0.953 ± 0.237
0.88AspAsn: 0.88 ± 0.241
6.085AspPro: 6.085 ± 0.597
1.539AspGln: 1.539 ± 0.335
5.425AspArg: 5.425 ± 0.87
2.639AspSer: 2.639 ± 0.471
4.032AspThr: 4.032 ± 0.554
4.325AspVal: 4.325 ± 0.655
0.806AspTrp: 0.806 ± 0.286
1.539AspTyr: 1.539 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
6.451GluAla: 6.451 ± 0.849
0.88GluCys: 0.88 ± 0.299
3.152GluAsp: 3.152 ± 0.506
1.979GluGlu: 1.979 ± 0.489
1.466GluPhe: 1.466 ± 0.291
3.519GluGly: 3.519 ± 0.455
1.759GluHis: 1.759 ± 0.462
1.759GluIle: 1.759 ± 0.436
0.953GluLys: 0.953 ± 0.277
6.158GluLeu: 6.158 ± 0.656
0.88GluMet: 0.88 ± 0.291
0.953GluAsn: 0.953 ± 0.333
4.765GluPro: 4.765 ± 1.163
3.226GluGln: 3.226 ± 0.481
4.912GluArg: 4.912 ± 0.778
2.932GluSer: 2.932 ± 0.474
3.079GluThr: 3.079 ± 0.395
3.885GluVal: 3.885 ± 0.898
1.759GluTrp: 1.759 ± 0.456
1.32GluTyr: 1.32 ± 0.253
0.0GluXaa: 0.0 ± 0.0
Phe
2.786PheAla: 2.786 ± 0.474
0.147PheCys: 0.147 ± 0.093
1.539PheAsp: 1.539 ± 0.375
1.466PheGlu: 1.466 ± 0.382
0.513PhePhe: 0.513 ± 0.202
2.346PheGly: 2.346 ± 0.472
0.293PheHis: 0.293 ± 0.15
0.806PheIle: 0.806 ± 0.239
0.367PheLys: 0.367 ± 0.188
1.906PheLeu: 1.906 ± 0.302
0.147PheMet: 0.147 ± 0.122
0.44PheAsn: 0.44 ± 0.149
1.026PhePro: 1.026 ± 0.274
0.367PheGln: 0.367 ± 0.155
1.979PheArg: 1.979 ± 0.445
0.733PheSer: 0.733 ± 0.285
1.32PheThr: 1.32 ± 0.342
1.979PheVal: 1.979 ± 0.416
0.22PheTrp: 0.22 ± 0.134
0.293PheTyr: 0.293 ± 0.173
0.0PheXaa: 0.0 ± 0.0
Gly
9.75GlyAla: 9.75 ± 1.378
1.173GlyCys: 1.173 ± 0.347
5.058GlyAsp: 5.058 ± 0.718
5.425GlyGlu: 5.425 ± 0.589
2.273GlyPhe: 2.273 ± 0.571
8.65GlyGly: 8.65 ± 1.323
1.466GlyHis: 1.466 ± 0.29
3.592GlyIle: 3.592 ± 0.518
2.273GlyLys: 2.273 ± 0.484
7.111GlyLeu: 7.111 ± 1.015
1.686GlyMet: 1.686 ± 0.327
1.466GlyAsn: 1.466 ± 0.339
4.985GlyPro: 4.985 ± 0.781
2.492GlyGln: 2.492 ± 0.473
7.844GlyArg: 7.844 ± 0.782
5.645GlySer: 5.645 ± 0.687
5.425GlyThr: 5.425 ± 0.821
7.184GlyVal: 7.184 ± 0.931
1.759GlyTrp: 1.759 ± 0.42
1.906GlyTyr: 1.906 ± 0.366
0.0GlyXaa: 0.0 ± 0.0
His
2.419HisAla: 2.419 ± 0.469
0.44HisCys: 0.44 ± 0.178
1.393HisAsp: 1.393 ± 0.37
0.953HisGlu: 0.953 ± 0.273
0.293HisPhe: 0.293 ± 0.143
2.053HisGly: 2.053 ± 0.48
0.66HisHis: 0.66 ± 0.246
0.733HisIle: 0.733 ± 0.199
0.513HisLys: 0.513 ± 0.2
1.393HisLeu: 1.393 ± 0.372
0.293HisMet: 0.293 ± 0.16
0.44HisAsn: 0.44 ± 0.183
2.639HisPro: 2.639 ± 0.745
0.073HisGln: 0.073 ± 0.078
1.906HisArg: 1.906 ± 0.628
1.393HisSer: 1.393 ± 0.344
1.466HisThr: 1.466 ± 0.301
1.393HisVal: 1.393 ± 0.303
0.293HisTrp: 0.293 ± 0.172
0.293HisTyr: 0.293 ± 0.15
0.0HisXaa: 0.0 ± 0.0
Ile
5.791IleAla: 5.791 ± 0.61
0.293IleCys: 0.293 ± 0.149
2.566IleAsp: 2.566 ± 0.398
3.445IleGlu: 3.445 ± 0.632
0.22IlePhe: 0.22 ± 0.112
3.885IleGly: 3.885 ± 0.506
0.513IleHis: 0.513 ± 0.2
1.173IleIle: 1.173 ± 0.296
1.173IleLys: 1.173 ± 0.394
1.759IleLeu: 1.759 ± 0.313
0.293IleMet: 0.293 ± 0.137
0.513IleAsn: 0.513 ± 0.237
2.859IlePro: 2.859 ± 0.349
0.367IleGln: 0.367 ± 0.15
2.492IleArg: 2.492 ± 0.397
1.32IleSer: 1.32 ± 0.294
3.519IleThr: 3.519 ± 0.503
2.786IleVal: 2.786 ± 0.461
0.147IleTrp: 0.147 ± 0.121
0.293IleTyr: 0.293 ± 0.134
0.0IleXaa: 0.0 ± 0.0
Lys
2.566LysAla: 2.566 ± 0.563
0.293LysCys: 0.293 ± 0.131
1.686LysAsp: 1.686 ± 0.391
0.733LysGlu: 0.733 ± 0.21
0.586LysPhe: 0.586 ± 0.217
1.833LysGly: 1.833 ± 0.348
0.513LysHis: 0.513 ± 0.201
1.026LysIle: 1.026 ± 0.238
0.806LysLys: 0.806 ± 0.241
2.053LysLeu: 2.053 ± 0.392
0.44LysMet: 0.44 ± 0.255
0.586LysAsn: 0.586 ± 0.209
1.979LysPro: 1.979 ± 0.659
0.586LysGln: 0.586 ± 0.241
1.686LysArg: 1.686 ± 0.384
1.759LysSer: 1.759 ± 0.515
1.393LysThr: 1.393 ± 0.324
2.419LysVal: 2.419 ± 0.66
0.44LysTrp: 0.44 ± 0.151
0.66LysTyr: 0.66 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
11.876LeuAla: 11.876 ± 1.072
0.733LeuCys: 0.733 ± 0.235
7.551LeuAsp: 7.551 ± 0.839
2.492LeuGlu: 2.492 ± 0.425
1.466LeuPhe: 1.466 ± 0.259
7.038LeuGly: 7.038 ± 0.76
2.053LeuHis: 2.053 ± 0.451
2.566LeuIle: 2.566 ± 0.332
1.613LeuLys: 1.613 ± 0.578
6.378LeuLeu: 6.378 ± 0.687
2.199LeuMet: 2.199 ± 0.412
1.613LeuAsn: 1.613 ± 0.397
5.278LeuPro: 5.278 ± 0.55
2.932LeuGln: 2.932 ± 0.533
9.164LeuArg: 9.164 ± 0.92
4.252LeuSer: 4.252 ± 0.664
5.498LeuThr: 5.498 ± 0.513
6.011LeuVal: 6.011 ± 0.745
2.492LeuTrp: 2.492 ± 0.473
1.173LeuTyr: 1.173 ± 0.368
0.0LeuXaa: 0.0 ± 0.0
Met
2.419MetAla: 2.419 ± 0.458
0.44MetCys: 0.44 ± 0.213
0.513MetAsp: 0.513 ± 0.22
0.586MetGlu: 0.586 ± 0.215
0.147MetPhe: 0.147 ± 0.085
1.686MetGly: 1.686 ± 0.434
0.367MetHis: 0.367 ± 0.239
0.513MetIle: 0.513 ± 0.191
0.367MetLys: 0.367 ± 0.155
1.246MetLeu: 1.246 ± 0.321
0.147MetMet: 0.147 ± 0.082
0.22MetAsn: 0.22 ± 0.126
2.273MetPro: 2.273 ± 0.449
0.66MetGln: 0.66 ± 0.218
1.906MetArg: 1.906 ± 0.408
1.173MetSer: 1.173 ± 0.313
1.32MetThr: 1.32 ± 0.284
1.026MetVal: 1.026 ± 0.277
0.586MetTrp: 0.586 ± 0.18
0.586MetTyr: 0.586 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
2.273AsnAla: 2.273 ± 0.439
0.293AsnCys: 0.293 ± 0.148
0.586AsnAsp: 0.586 ± 0.187
0.586AsnGlu: 0.586 ± 0.193
0.44AsnPhe: 0.44 ± 0.147
1.466AsnGly: 1.466 ± 0.318
0.147AsnHis: 0.147 ± 0.118
0.44AsnIle: 0.44 ± 0.164
0.513AsnLys: 0.513 ± 0.186
1.466AsnLeu: 1.466 ± 0.265
0.073AsnMet: 0.073 ± 0.085
0.293AsnAsn: 0.293 ± 0.135
1.686AsnPro: 1.686 ± 0.4
0.44AsnGln: 0.44 ± 0.167
1.613AsnArg: 1.613 ± 0.363
0.586AsnSer: 0.586 ± 0.223
1.1AsnThr: 1.1 ± 0.321
1.173AsnVal: 1.173 ± 0.332
0.44AsnTrp: 0.44 ± 0.183
0.0AsnTyr: 0.0 ± 0.0
0.0AsnXaa: 0.0 ± 0.0
Pro
10.263ProAla: 10.263 ± 1.286
0.88ProCys: 0.88 ± 0.274
5.352ProAsp: 5.352 ± 0.815
6.158ProGlu: 6.158 ± 1.398
1.613ProPhe: 1.613 ± 0.351
5.791ProGly: 5.791 ± 0.752
1.613ProHis: 1.613 ± 0.475
2.199ProIle: 2.199 ± 0.459
1.759ProLys: 1.759 ± 0.398
6.231ProLeu: 6.231 ± 0.734
1.32ProMet: 1.32 ± 0.381
0.88ProAsn: 0.88 ± 0.279
4.545ProPro: 4.545 ± 0.911
1.833ProGln: 1.833 ± 0.413
6.305ProArg: 6.305 ± 1.12
3.519ProSer: 3.519 ± 0.544
4.472ProThr: 4.472 ± 0.486
6.158ProVal: 6.158 ± 0.676
1.539ProTrp: 1.539 ± 0.393
1.613ProTyr: 1.613 ± 0.435
0.0ProXaa: 0.0 ± 0.0
Gln
4.765GlnAla: 4.765 ± 0.715
0.367GlnCys: 0.367 ± 0.161
1.613GlnAsp: 1.613 ± 0.348
0.88GlnGlu: 0.88 ± 0.21
1.026GlnPhe: 1.026 ± 0.245
1.173GlnGly: 1.173 ± 0.359
0.513GlnHis: 0.513 ± 0.236
1.1GlnIle: 1.1 ± 0.301
0.586GlnLys: 0.586 ± 0.197
3.885GlnLeu: 3.885 ± 0.575
0.513GlnMet: 0.513 ± 0.211
0.293GlnAsn: 0.293 ± 0.15
2.346GlnPro: 2.346 ± 0.634
0.806GlnGln: 0.806 ± 0.195
2.932GlnArg: 2.932 ± 0.581
1.906GlnSer: 1.906 ± 0.411
1.393GlnThr: 1.393 ± 0.319
2.566GlnVal: 2.566 ± 0.37
0.513GlnTrp: 0.513 ± 0.186
0.586GlnTyr: 0.586 ± 0.202
0.0GlnXaa: 0.0 ± 0.0
Arg
10.336ArgAla: 10.336 ± 1.434
0.586ArgCys: 0.586 ± 0.234
4.179ArgAsp: 4.179 ± 0.503
4.618ArgGlu: 4.618 ± 0.62
1.906ArgPhe: 1.906 ± 0.326
7.331ArgGly: 7.331 ± 0.676
2.053ArgHis: 2.053 ± 0.449
4.179ArgIle: 4.179 ± 0.58
2.126ArgLys: 2.126 ± 0.372
8.211ArgLeu: 8.211 ± 0.933
1.613ArgMet: 1.613 ± 0.334
1.246ArgAsn: 1.246 ± 0.386
6.524ArgPro: 6.524 ± 1.001
3.812ArgGln: 3.812 ± 0.554
11.363ArgArg: 11.363 ± 2.152
4.912ArgSer: 4.912 ± 0.761
7.258ArgThr: 7.258 ± 1.023
6.231ArgVal: 6.231 ± 0.773
1.906ArgTrp: 1.906 ± 0.33
1.979ArgTyr: 1.979 ± 0.358
0.0ArgXaa: 0.0 ± 0.0
Ser
6.964SerAla: 6.964 ± 0.986
0.22SerCys: 0.22 ± 0.105
3.152SerAsp: 3.152 ± 0.521
2.126SerGlu: 2.126 ± 0.438
0.733SerPhe: 0.733 ± 0.215
4.692SerGly: 4.692 ± 0.739
1.393SerHis: 1.393 ± 0.348
1.32SerIle: 1.32 ± 0.336
1.466SerLys: 1.466 ± 0.306
3.812SerLeu: 3.812 ± 0.506
1.833SerMet: 1.833 ± 0.389
1.026SerAsn: 1.026 ± 0.295
3.959SerPro: 3.959 ± 0.422
1.173SerGln: 1.173 ± 0.318
3.885SerArg: 3.885 ± 0.559
3.079SerSer: 3.079 ± 0.572
3.739SerThr: 3.739 ± 0.556
3.959SerVal: 3.959 ± 0.551
1.246SerTrp: 1.246 ± 0.371
1.026SerTyr: 1.026 ± 0.279
0.0SerXaa: 0.0 ± 0.0
Thr
9.823ThrAla: 9.823 ± 0.879
0.513ThrCys: 0.513 ± 0.207
3.665ThrAsp: 3.665 ± 0.622
4.472ThrGlu: 4.472 ± 0.637
1.466ThrPhe: 1.466 ± 0.325
6.378ThrGly: 6.378 ± 0.504
0.953ThrHis: 0.953 ± 0.3
2.273ThrIle: 2.273 ± 0.536
1.246ThrLys: 1.246 ± 0.541
5.718ThrLeu: 5.718 ± 0.828
1.1ThrMet: 1.1 ± 0.23
0.953ThrAsn: 0.953 ± 0.227
5.058ThrPro: 5.058 ± 0.739
1.613ThrGln: 1.613 ± 0.325
5.132ThrArg: 5.132 ± 0.864
2.712ThrSer: 2.712 ± 0.507
2.932ThrThr: 2.932 ± 0.55
4.765ThrVal: 4.765 ± 0.734
1.32ThrTrp: 1.32 ± 0.301
1.1ThrTyr: 1.1 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
9.164ValAla: 9.164 ± 0.753
0.88ValCys: 0.88 ± 0.297
4.985ValAsp: 4.985 ± 0.583
6.231ValGlu: 6.231 ± 1.033
1.466ValPhe: 1.466 ± 0.485
6.011ValGly: 6.011 ± 0.894
1.979ValHis: 1.979 ± 0.438
3.665ValIle: 3.665 ± 0.502
1.686ValLys: 1.686 ± 0.388
6.671ValLeu: 6.671 ± 0.719
1.393ValMet: 1.393 ± 0.299
1.32ValAsn: 1.32 ± 0.301
6.011ValPro: 6.011 ± 0.845
1.833ValGln: 1.833 ± 0.501
5.645ValArg: 5.645 ± 0.65
3.372ValSer: 3.372 ± 0.515
4.545ValThr: 4.545 ± 0.659
5.498ValVal: 5.498 ± 0.817
1.906ValTrp: 1.906 ± 0.398
1.466ValTyr: 1.466 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
2.199TrpAla: 2.199 ± 0.489
0.147TrpCys: 0.147 ± 0.117
1.32TrpAsp: 1.32 ± 0.413
1.173TrpGlu: 1.173 ± 0.322
0.22TrpPhe: 0.22 ± 0.136
1.539TrpGly: 1.539 ± 0.308
0.66TrpHis: 0.66 ± 0.205
0.953TrpIle: 0.953 ± 0.238
0.66TrpLys: 0.66 ± 0.205
2.566TrpLeu: 2.566 ± 0.451
0.367TrpMet: 0.367 ± 0.163
0.293TrpAsn: 0.293 ± 0.13
1.393TrpPro: 1.393 ± 0.404
1.1TrpGln: 1.1 ± 0.33
2.199TrpArg: 2.199 ± 0.403
0.733TrpSer: 0.733 ± 0.224
1.686TrpThr: 1.686 ± 0.462
1.246TrpVal: 1.246 ± 0.307
0.733TrpTrp: 0.733 ± 0.237
0.293TrpTyr: 0.293 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.445TyrAla: 3.445 ± 0.425
0.367TyrCys: 0.367 ± 0.148
1.466TyrAsp: 1.466 ± 0.281
0.953TyrGlu: 0.953 ± 0.262
0.513TyrPhe: 0.513 ± 0.191
2.053TyrGly: 2.053 ± 0.389
0.073TyrHis: 0.073 ± 0.063
0.367TyrIle: 0.367 ± 0.134
0.44TyrLys: 0.44 ± 0.205
1.613TyrLeu: 1.613 ± 0.395
0.293TyrMet: 0.293 ± 0.149
0.22TyrAsn: 0.22 ± 0.113
1.026TyrPro: 1.026 ± 0.324
0.44TyrGln: 0.44 ± 0.15
1.833TyrArg: 1.833 ± 0.381
0.953TyrSer: 0.953 ± 0.246
0.733TyrThr: 0.733 ± 0.226
1.466TyrVal: 1.466 ± 0.312
0.22TyrTrp: 0.22 ± 0.135
0.367TyrTyr: 0.367 ± 0.139
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (13642 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski