Mycobacterium phage PhrostyMug

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T2A9G4|T2A9G4_9CAUD Structural protein OS=Mycobacterium phage PhrostyMug OX=1354512 GN=PHROSTYMUG_32 PE=4 SV=1
MM1 pKa = 7.61GFTLRR6 pKa = 11.84LFGIPVLGLEE16 pKa = 4.12ITGDD20 pKa = 3.77GSAEE24 pKa = 3.94EE25 pKa = 4.62YY26 pKa = 10.64INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T2A8Q2|T2A8Q2_9CAUD Uncharacterized protein OS=Mycobacterium phage PhrostyMug OX=1354512 GN=PHROSTYMUG_47 PE=4 SV=1
MM1 pKa = 7.58SKK3 pKa = 10.44HH4 pKa = 6.12EE5 pKa = 4.3YY6 pKa = 8.17VTEE9 pKa = 4.26LGGEE13 pKa = 4.12LWVQEE18 pKa = 3.98HH19 pKa = 6.47LARR22 pKa = 11.84RR23 pKa = 11.84LGLTRR28 pKa = 11.84VRR30 pKa = 11.84EE31 pKa = 4.51SVSGRR36 pKa = 11.84HH37 pKa = 5.61RR38 pKa = 11.84LPDD41 pKa = 3.18VRR43 pKa = 11.84FSQEE47 pKa = 3.84LPGGTVYY54 pKa = 10.56WSVNRR59 pKa = 11.84KK60 pKa = 8.97NFFRR64 pKa = 11.84RR65 pKa = 11.84DD66 pKa = 3.31DD67 pKa = 3.91SLPSGWVQRR76 pKa = 11.84IYY78 pKa = 10.86PRR80 pKa = 11.84VATSFRR86 pKa = 11.84TAEE89 pKa = 3.85

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

16724

21

826

172.4

18.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.603 ± 0.428

0.783 ± 0.13

6.512 ± 0.216

6.565 ± 0.242

3.085 ± 0.18

8.461 ± 0.367

1.901 ± 0.159

4.849 ± 0.221

4.186 ± 0.244

8.497 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.116

3.151 ± 0.137

5.734 ± 0.253

3.259 ± 0.136

6.775 ± 0.399

5.89 ± 0.285

6.53 ± 0.287

7.283 ± 0.241

2.009 ± 0.115

2.84 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski