Mycobacterium phage SamScheppers

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4AQU6|A0A2R4AQU6_9CAUD Uncharacterized protein OS=Mycobacterium phage SamScheppers OX=2126955 GN=85 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84YY3 pKa = 9.54GVAFQTAYY11 pKa = 10.99SDD13 pKa = 3.57LGGTTEE19 pKa = 4.09VIEE22 pKa = 4.64FEE24 pKa = 4.43SEE26 pKa = 3.59QAAQAFIDD34 pKa = 4.09TVQPGVGVVLTLVRR48 pKa = 11.84FDD50 pKa = 3.56YY51 pKa = 10.13STYY54 pKa = 9.36EE55 pKa = 4.1TIGG58 pKa = 3.21

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4AQQ9|A0A2R4AQQ9_9CAUD SprT-like protease OS=Mycobacterium phage SamScheppers OX=2126955 GN=80 PE=4 SV=1
MM1 pKa = 7.55GHH3 pKa = 5.5RR4 pKa = 11.84QKK6 pKa = 11.44GKK8 pKa = 7.64GCKK11 pKa = 9.47RR12 pKa = 11.84KK13 pKa = 10.06RR14 pKa = 11.84SWHH17 pKa = 5.81RR18 pKa = 11.84LGNQDD23 pKa = 2.84RR24 pKa = 11.84LYY26 pKa = 10.86RR27 pKa = 11.84RR28 pKa = 11.84AIEE31 pKa = 4.55ALDD34 pKa = 3.89AAQQRR39 pKa = 11.84AIEE42 pKa = 4.14YY43 pKa = 10.81AEE45 pKa = 3.88VDD47 pKa = 3.19DD48 pKa = 5.05RR49 pKa = 11.84EE50 pKa = 4.43VVRR53 pKa = 11.84RR54 pKa = 11.84GAA56 pKa = 3.36

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

18614

27

1395

198.0

21.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.426 ± 0.465

1.123 ± 0.148

6.662 ± 0.264

5.969 ± 0.295

2.735 ± 0.155

8.569 ± 0.44

2.074 ± 0.18

3.89 ± 0.236

3.315 ± 0.188

8.327 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.886 ± 0.109

2.928 ± 0.195

5.85 ± 0.236

3.524 ± 0.173

7.806 ± 0.495

4.948 ± 0.249

6.033 ± 0.24

7.527 ± 0.228

1.907 ± 0.091

2.503 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski