Sclerotinia sclerotiorum mitovirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus; unclassified Mitovirus

Average proteome isoelectric point is 8.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2EQQ4|H2EQQ4_9VIRU RNA-dependent RNA polymerases OS=Sclerotinia sclerotiorum mitovirus 2 OX=1133728 GN=SsMV2_gp1 PE=4 SV=1
MM1 pKa = 6.89TQPLVLKK8 pKa = 10.31LMKK11 pKa = 9.52WVRR14 pKa = 11.84KK15 pKa = 9.24YY16 pKa = 10.61YY17 pKa = 10.92YY18 pKa = 10.3KK19 pKa = 10.34GTSPNLAAFTRR30 pKa = 11.84HH31 pKa = 6.34AIHH34 pKa = 7.38LFFLWGKK41 pKa = 7.16TKK43 pKa = 9.66GWKK46 pKa = 6.47WTATAFKK53 pKa = 9.25LTRR56 pKa = 11.84LAITRR61 pKa = 11.84TLAGQEE67 pKa = 3.97LPRR70 pKa = 11.84PFGISLCKK78 pKa = 9.36KK79 pKa = 8.23TNLPMMLPLQVRR91 pKa = 11.84LAILRR96 pKa = 11.84KK97 pKa = 9.69DD98 pKa = 3.6FLLISYY104 pKa = 9.31VLTVLQLSRR113 pKa = 11.84LILGTGVIEE122 pKa = 4.74SYY124 pKa = 11.46DD125 pKa = 4.05SITRR129 pKa = 11.84PCTGSNPFTGASVSIVMKK147 pKa = 10.62RR148 pKa = 11.84MGIRR152 pKa = 11.84RR153 pKa = 11.84LDD155 pKa = 3.61EE156 pKa = 4.15PTSFTFPWVSTSGPNGISIATSFSDD181 pKa = 5.24LMNIPQPILEE191 pKa = 4.41CLYY194 pKa = 10.39TLGGPEE200 pKa = 4.17FKK202 pKa = 10.64EE203 pKa = 4.19KK204 pKa = 10.78VSSLKK209 pKa = 10.96GFVEE213 pKa = 4.61TTNGLLWKK221 pKa = 8.83LTKK224 pKa = 10.23LDD226 pKa = 3.8PDD228 pKa = 3.32KK229 pKa = 11.18TGNLLRR235 pKa = 11.84RR236 pKa = 11.84ISIKK240 pKa = 9.94KK241 pKa = 9.15DD242 pKa = 2.89KK243 pKa = 9.76EE244 pKa = 4.15YY245 pKa = 10.85KK246 pKa = 9.9SRR248 pKa = 11.84PFAIVDD254 pKa = 3.93YY255 pKa = 8.89ITQSALTPLHH265 pKa = 6.46DD266 pKa = 3.38RR267 pKa = 11.84LYY269 pKa = 10.35RR270 pKa = 11.84VLGSIPQDD278 pKa = 3.31CTFDD282 pKa = 3.49QNKK285 pKa = 9.3GFKK288 pKa = 10.18DD289 pKa = 3.49LLYY292 pKa = 11.07GGGPYY297 pKa = 10.63YY298 pKa = 11.11SFDD301 pKa = 3.51LTSATDD307 pKa = 3.65RR308 pKa = 11.84FPIFVQEE315 pKa = 4.98MVLAWLTSEE324 pKa = 4.48QYY326 pKa = 11.27ASAWVQAMVGIPFSTPNGPEE346 pKa = 3.78VEE348 pKa = 5.03FKK350 pKa = 10.81CGQPLGAKK358 pKa = 9.83SSWAMFTLSHH368 pKa = 6.42HH369 pKa = 6.58FVVQYY374 pKa = 10.63CAMVLNIDD382 pKa = 3.61NPRR385 pKa = 11.84YY386 pKa = 9.97KK387 pKa = 10.33ILGDD391 pKa = 4.46DD392 pKa = 4.66IVICDD397 pKa = 3.45HH398 pKa = 6.99ALAAKK403 pKa = 9.21YY404 pKa = 10.51LEE406 pKa = 4.38VMSQLGVEE414 pKa = 4.15ISSVKK419 pKa = 8.97THH421 pKa = 5.2VSEE424 pKa = 4.97NLFEE428 pKa = 4.05FAKK431 pKa = 10.57RR432 pKa = 11.84FGLQSVEE439 pKa = 3.69ISSFPITALVSDD451 pKa = 4.01INNYY455 pKa = 9.3VSIVATLATTAVEE468 pKa = 4.26RR469 pKa = 11.84GNLPLFVSGNTPRR482 pKa = 11.84FWEE485 pKa = 4.16SLLKK489 pKa = 10.78KK490 pKa = 9.49EE491 pKa = 4.81DD492 pKa = 3.4KK493 pKa = 10.37PRR495 pKa = 11.84LVNNLVHH502 pKa = 6.66SARR505 pKa = 11.84LLSYY509 pKa = 11.27LLMSNKK515 pKa = 9.6PYY517 pKa = 10.82LVAQSDD523 pKa = 4.08LLRR526 pKa = 11.84FAEE529 pKa = 4.21AAGFGGLAISQVFAAYY545 pKa = 9.2KK546 pKa = 10.15RR547 pKa = 11.84AVLYY551 pKa = 10.08MKK553 pKa = 10.52DD554 pKa = 3.55RR555 pKa = 11.84EE556 pKa = 4.08IDD558 pKa = 3.34KK559 pKa = 10.74LADD562 pKa = 3.0MSMRR566 pKa = 11.84FIRR569 pKa = 11.84PVQSMLSQIMFLPWRR584 pKa = 11.84GRR586 pKa = 11.84VTVDD590 pKa = 2.9YY591 pKa = 10.77RR592 pKa = 11.84KK593 pKa = 9.93FIPIIDD599 pKa = 4.58SIDD602 pKa = 3.83SNKK605 pKa = 10.13EE606 pKa = 3.46LYY608 pKa = 10.79LSFRR612 pKa = 11.84EE613 pKa = 4.19EE614 pKa = 3.87FEE616 pKa = 4.28KK617 pKa = 11.14ADD619 pKa = 5.3DD620 pKa = 4.09LVTLNKK626 pKa = 9.96VLDD629 pKa = 3.92DD630 pKa = 3.88HH631 pKa = 7.5PIRR634 pKa = 11.84PMPNLNGLQPNRR646 pKa = 11.84PKK648 pKa = 10.95LLVGQTRR655 pKa = 11.84AALIRR660 pKa = 11.84ALVKK664 pKa = 10.35EE665 pKa = 4.24LTMLAKK671 pKa = 10.72GEE673 pKa = 4.09EE674 pKa = 4.39PKK676 pKa = 11.0

Molecular weight: 76.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2EQQ4|H2EQQ4_9VIRU RNA-dependent RNA polymerases OS=Sclerotinia sclerotiorum mitovirus 2 OX=1133728 GN=SsMV2_gp1 PE=4 SV=1
MM1 pKa = 6.89TQPLVLKK8 pKa = 10.31LMKK11 pKa = 9.52WVRR14 pKa = 11.84KK15 pKa = 9.24YY16 pKa = 10.61YY17 pKa = 10.92YY18 pKa = 10.3KK19 pKa = 10.34GTSPNLAAFTRR30 pKa = 11.84HH31 pKa = 6.34AIHH34 pKa = 7.38LFFLWGKK41 pKa = 7.16TKK43 pKa = 9.66GWKK46 pKa = 6.47WTATAFKK53 pKa = 9.25LTRR56 pKa = 11.84LAITRR61 pKa = 11.84TLAGQEE67 pKa = 3.97LPRR70 pKa = 11.84PFGISLCKK78 pKa = 9.36KK79 pKa = 8.23TNLPMMLPLQVRR91 pKa = 11.84LAILRR96 pKa = 11.84KK97 pKa = 9.69DD98 pKa = 3.6FLLISYY104 pKa = 9.31VLTVLQLSRR113 pKa = 11.84LILGTGVIEE122 pKa = 4.74SYY124 pKa = 11.46DD125 pKa = 4.05SITRR129 pKa = 11.84PCTGSNPFTGASVSIVMKK147 pKa = 10.62RR148 pKa = 11.84MGIRR152 pKa = 11.84RR153 pKa = 11.84LDD155 pKa = 3.61EE156 pKa = 4.15PTSFTFPWVSTSGPNGISIATSFSDD181 pKa = 5.24LMNIPQPILEE191 pKa = 4.41CLYY194 pKa = 10.39TLGGPEE200 pKa = 4.17FKK202 pKa = 10.64EE203 pKa = 4.19KK204 pKa = 10.78VSSLKK209 pKa = 10.96GFVEE213 pKa = 4.61TTNGLLWKK221 pKa = 8.83LTKK224 pKa = 10.23LDD226 pKa = 3.8PDD228 pKa = 3.32KK229 pKa = 11.18TGNLLRR235 pKa = 11.84RR236 pKa = 11.84ISIKK240 pKa = 9.94KK241 pKa = 9.15DD242 pKa = 2.89KK243 pKa = 9.76EE244 pKa = 4.15YY245 pKa = 10.85KK246 pKa = 9.9SRR248 pKa = 11.84PFAIVDD254 pKa = 3.93YY255 pKa = 8.89ITQSALTPLHH265 pKa = 6.46DD266 pKa = 3.38RR267 pKa = 11.84LYY269 pKa = 10.35RR270 pKa = 11.84VLGSIPQDD278 pKa = 3.31CTFDD282 pKa = 3.49QNKK285 pKa = 9.3GFKK288 pKa = 10.18DD289 pKa = 3.49LLYY292 pKa = 11.07GGGPYY297 pKa = 10.63YY298 pKa = 11.11SFDD301 pKa = 3.51LTSATDD307 pKa = 3.65RR308 pKa = 11.84FPIFVQEE315 pKa = 4.98MVLAWLTSEE324 pKa = 4.48QYY326 pKa = 11.27ASAWVQAMVGIPFSTPNGPEE346 pKa = 3.78VEE348 pKa = 5.03FKK350 pKa = 10.81CGQPLGAKK358 pKa = 9.83SSWAMFTLSHH368 pKa = 6.42HH369 pKa = 6.58FVVQYY374 pKa = 10.63CAMVLNIDD382 pKa = 3.61NPRR385 pKa = 11.84YY386 pKa = 9.97KK387 pKa = 10.33ILGDD391 pKa = 4.46DD392 pKa = 4.66IVICDD397 pKa = 3.45HH398 pKa = 6.99ALAAKK403 pKa = 9.21YY404 pKa = 10.51LEE406 pKa = 4.38VMSQLGVEE414 pKa = 4.15ISSVKK419 pKa = 8.97THH421 pKa = 5.2VSEE424 pKa = 4.97NLFEE428 pKa = 4.05FAKK431 pKa = 10.57RR432 pKa = 11.84FGLQSVEE439 pKa = 3.69ISSFPITALVSDD451 pKa = 4.01INNYY455 pKa = 9.3VSIVATLATTAVEE468 pKa = 4.26RR469 pKa = 11.84GNLPLFVSGNTPRR482 pKa = 11.84FWEE485 pKa = 4.16SLLKK489 pKa = 10.78KK490 pKa = 9.49EE491 pKa = 4.81DD492 pKa = 3.4KK493 pKa = 10.37PRR495 pKa = 11.84LVNNLVHH502 pKa = 6.66SARR505 pKa = 11.84LLSYY509 pKa = 11.27LLMSNKK515 pKa = 9.6PYY517 pKa = 10.82LVAQSDD523 pKa = 4.08LLRR526 pKa = 11.84FAEE529 pKa = 4.21AAGFGGLAISQVFAAYY545 pKa = 9.2KK546 pKa = 10.15RR547 pKa = 11.84AVLYY551 pKa = 10.08MKK553 pKa = 10.52DD554 pKa = 3.55RR555 pKa = 11.84EE556 pKa = 4.08IDD558 pKa = 3.34KK559 pKa = 10.74LADD562 pKa = 3.0MSMRR566 pKa = 11.84FIRR569 pKa = 11.84PVQSMLSQIMFLPWRR584 pKa = 11.84GRR586 pKa = 11.84VTVDD590 pKa = 2.9YY591 pKa = 10.77RR592 pKa = 11.84KK593 pKa = 9.93FIPIIDD599 pKa = 4.58SIDD602 pKa = 3.83SNKK605 pKa = 10.13EE606 pKa = 3.46LYY608 pKa = 10.79LSFRR612 pKa = 11.84EE613 pKa = 4.19EE614 pKa = 3.87FEE616 pKa = 4.28KK617 pKa = 11.14ADD619 pKa = 5.3DD620 pKa = 4.09LVTLNKK626 pKa = 9.96VLDD629 pKa = 3.92DD630 pKa = 3.88HH631 pKa = 7.5PIRR634 pKa = 11.84PMPNLNGLQPNRR646 pKa = 11.84PKK648 pKa = 10.95LLVGQTRR655 pKa = 11.84AALIRR660 pKa = 11.84ALVKK664 pKa = 10.35EE665 pKa = 4.24LTMLAKK671 pKa = 10.72GEE673 pKa = 4.09EE674 pKa = 4.39PKK676 pKa = 11.0

Molecular weight: 76.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

676

676

676

676.0

76.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.361 ± 0.0

1.036 ± 0.0

4.586 ± 0.0

4.29 ± 0.0

5.325 ± 0.0

5.621 ± 0.0

1.331 ± 0.0

5.917 ± 0.0

6.657 ± 0.0

12.722 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.959 ± 0.0

3.55 ± 0.0

5.769 ± 0.0

3.107 ± 0.0

5.473 ± 0.0

7.544 ± 0.0

6.213 ± 0.0

6.509 ± 0.0

1.627 ± 0.0

3.402 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski