Streptococcus phage Javan446

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B3I1|A0A4D6B3I1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan446 OX=2548172 GN=Javan446_0021 PE=4 SV=1
MM1 pKa = 7.56AKK3 pKa = 9.45IDD5 pKa = 4.44IIDD8 pKa = 3.77NYY10 pKa = 8.7EE11 pKa = 3.83TLLISVEE18 pKa = 4.2EE19 pKa = 4.01IRR21 pKa = 11.84ASLEE25 pKa = 3.87SLYY28 pKa = 11.29AWLDD32 pKa = 3.24KK33 pKa = 11.25DD34 pKa = 4.45IDD36 pKa = 3.8WDD38 pKa = 4.35SQCDD42 pKa = 3.35WYY44 pKa = 10.84DD45 pKa = 4.29FISQHH50 pKa = 5.75SSQIAILNLIMYY62 pKa = 9.65RR63 pKa = 11.84LDD65 pKa = 3.44SLEE68 pKa = 3.94VEE70 pKa = 4.13HH71 pKa = 7.25RR72 pKa = 11.84SVIEE76 pKa = 3.64NTMKK80 pKa = 10.92GKK82 pKa = 10.21

Molecular weight: 9.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B4A2|A0A4D6B4A2_9CAUD Lysin OS=Streptococcus phage Javan446 OX=2548172 GN=Javan446_0006 PE=4 SV=1
MM1 pKa = 7.15TFLMVVSYY9 pKa = 9.42TVLPHH14 pKa = 5.57AQSRR18 pKa = 11.84QTLRR22 pKa = 11.84AWGFFIFLKK31 pKa = 10.07QKK33 pKa = 9.98RR34 pKa = 11.84ALIRR38 pKa = 11.84KK39 pKa = 7.71QALTVEE45 pKa = 4.62LDD47 pKa = 3.54TPKK50 pKa = 11.07

Molecular weight: 5.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13722

37

1211

201.8

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.945 ± 0.985

0.598 ± 0.1

6.53 ± 0.405

7.113 ± 0.605

4.271 ± 0.18

6.253 ± 0.507

1.596 ± 0.164

7.302 ± 0.212

9.058 ± 0.424

8.468 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.166

5.276 ± 0.274

2.325 ± 0.155

4.059 ± 0.229

4.125 ± 0.285

6.355 ± 0.482

6.369 ± 0.244

6.216 ± 0.244

1.1 ± 0.142

3.556 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski