Psychrobacter phage Psymv2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3ENA4|G3ENA4_9CAUD Hypothetical phage protein OS=Psychrobacter phage Psymv2 OX=1071177 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.33KK3 pKa = 8.67VLKK6 pKa = 9.92WIGIVVIALIAIGFIGAMLGDD27 pKa = 4.48DD28 pKa = 4.03TDD30 pKa = 3.99STSTSDD36 pKa = 4.39TSLNDD41 pKa = 3.13NNTVSTEE48 pKa = 4.14TEE50 pKa = 4.06AVPEE54 pKa = 4.62DD55 pKa = 3.66NSMTAQQKK63 pKa = 9.21NAVRR67 pKa = 11.84SVKK70 pKa = 10.63NYY72 pKa = 9.73IGFAGFSRR80 pKa = 11.84DD81 pKa = 3.22GLINQLSSDD90 pKa = 3.83AGDD93 pKa = 4.6GYY95 pKa = 11.37NIDD98 pKa = 4.36DD99 pKa = 3.7ATIAVDD105 pKa = 5.19SMDD108 pKa = 3.85IDD110 pKa = 4.13FNEE113 pKa = 4.26QAGKK117 pKa = 9.49SAKK120 pKa = 10.33NYY122 pKa = 10.09ISIQGFSCDD131 pKa = 3.33GLINQLSSPAGDD143 pKa = 3.82KK144 pKa = 8.72YY145 pKa = 9.58TKK147 pKa = 10.1EE148 pKa = 3.74QAEE151 pKa = 4.44YY152 pKa = 9.97GAKK155 pKa = 10.37LAGACDD161 pKa = 3.43

Molecular weight: 17.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3EN88|G3EN88_9CAUD Hypothetical phage protein OS=Psychrobacter phage Psymv2 OX=1071177 PE=4 SV=1
MM1 pKa = 6.77NTNLITGFKK10 pKa = 10.35EE11 pKa = 3.93RR12 pKa = 11.84INLCRR17 pKa = 11.84HH18 pKa = 5.97LANRR22 pKa = 11.84HH23 pKa = 4.95PAWVGRR29 pKa = 11.84VEE31 pKa = 4.2MNSYY35 pKa = 8.7LTGCSRR41 pKa = 11.84THH43 pKa = 5.83EE44 pKa = 4.29RR45 pKa = 11.84LLSGLVKK52 pKa = 10.1IGYY55 pKa = 8.71LEE57 pKa = 4.43RR58 pKa = 11.84SNTSPAGWRR67 pKa = 11.84VVKK70 pKa = 10.81SKK72 pKa = 11.34VKK74 pKa = 10.35GFKK77 pKa = 10.54ALL79 pKa = 3.47

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11099

68

1503

226.5

25.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.83 ± 0.72

0.91 ± 0.197

6.451 ± 0.389

5.712 ± 0.267

3.361 ± 0.238

6.514 ± 0.366

1.982 ± 0.35

6.325 ± 0.337

6.127 ± 0.411

8.046 ± 0.366

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.973 ± 0.208

5.577 ± 0.216

3.334 ± 0.264

4.136 ± 0.215

4.64 ± 0.287

6.775 ± 0.35

6.712 ± 0.702

6.334 ± 0.249

1.27 ± 0.139

2.991 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski