Rhodococcus jostii (strain RHA1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; Rhodococcus jostii

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0S6K0|Q0S6K0_RHOJR Uncharacterized protein OS=Rhodococcus jostii (strain RHA1) OX=101510 GN=RHA1_ro05055 PE=4 SV=1
MM1 pKa = 8.23RR2 pKa = 11.84DD3 pKa = 2.8TAIAVDD9 pKa = 4.45TIGVATVISEE19 pKa = 3.76EE20 pKa = 4.14RR21 pKa = 11.84VADD24 pKa = 3.57GHH26 pKa = 5.59LTEE29 pKa = 5.33GSDD32 pKa = 3.21MTGLVRR38 pKa = 11.84RR39 pKa = 11.84VCTAVVALSATAVLVAGCGSDD60 pKa = 3.43TTPTAVPEE68 pKa = 4.48SGASAATTSSEE79 pKa = 4.01STTVIDD85 pKa = 4.1GQEE88 pKa = 4.22GEE90 pKa = 4.47DD91 pKa = 3.6AGGDD95 pKa = 3.27VDD97 pKa = 5.34FEE99 pKa = 4.51VAIGEE104 pKa = 4.42CVKK107 pKa = 11.09LGGTVSDD114 pKa = 4.22AEE116 pKa = 4.07IDD118 pKa = 3.71KK119 pKa = 10.7AVCGSADD126 pKa = 3.31SNYY129 pKa = 10.61KK130 pKa = 10.45VIAKK134 pKa = 9.33AAKK137 pKa = 9.55NSQCISDD144 pKa = 3.84ADD146 pKa = 3.64SYY148 pKa = 11.75YY149 pKa = 10.63YY150 pKa = 8.66EE151 pKa = 4.14TLGGIEE157 pKa = 4.08QGAICLDD164 pKa = 3.75VDD166 pKa = 3.83WVIGGCMDD174 pKa = 4.44VGGEE178 pKa = 3.92DD179 pKa = 4.28PARR182 pKa = 11.84IDD184 pKa = 4.56CGDD187 pKa = 3.41TTAVDD192 pKa = 4.14GVKK195 pKa = 9.01VTEE198 pKa = 4.22IVQGATSVDD207 pKa = 3.6SCSTSSNGYY216 pKa = 9.2EE217 pKa = 3.83YY218 pKa = 10.24PEE220 pKa = 4.15RR221 pKa = 11.84KK222 pKa = 9.12FVVCVDD228 pKa = 3.62EE229 pKa = 4.47LL230 pKa = 3.79

Molecular weight: 23.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0SFB2|Q0SFB2_RHOJR Possible membrane protein OS=Rhodococcus jostii (strain RHA1) OX=101510 GN=RHA1_ro01963 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9075

0

9075

2871138

24

11258

316.4

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.473 ± 0.035

0.867 ± 0.009

6.266 ± 0.024

5.464 ± 0.025

3.024 ± 0.016

8.903 ± 0.028

2.348 ± 0.014

4.181 ± 0.021

2.083 ± 0.022

9.779 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.012

2.072 ± 0.014

5.766 ± 0.021

2.812 ± 0.013

7.579 ± 0.032

5.827 ± 0.016

6.392 ± 0.021

8.785 ± 0.031

1.442 ± 0.011

2.019 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski