Staphylococcus phage BP39

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Rakietenvirinae; Rosenblumvirus; Staphylococcus virus BP39

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A185AMV8|A0A185AMV8_9CAUD Uncharacterized protein OS=Staphylococcus phage BP39 OX=1543206 GN=BP39_03 PE=4 SV=1
MM1 pKa = 7.27VNVDD5 pKa = 3.72NAPEE9 pKa = 4.15EE10 pKa = 4.21KK11 pKa = 10.3GQAYY15 pKa = 8.38TEE17 pKa = 3.92MLQLFNKK24 pKa = 9.67LIQWNPAYY32 pKa = 9.81TFDD35 pKa = 3.95NAINLVSACQQLLLNYY51 pKa = 9.28NSSVVKK57 pKa = 9.87FLNDD61 pKa = 3.22EE62 pKa = 4.62LNNEE66 pKa = 4.41TKK68 pKa = 10.41PEE70 pKa = 4.48SILSYY75 pKa = 10.51IAGDD79 pKa = 4.14DD80 pKa = 5.49SIEE83 pKa = 3.7QWNMHH88 pKa = 5.76KK89 pKa = 10.66GFYY92 pKa = 7.63EE93 pKa = 4.27TYY95 pKa = 9.67NVYY98 pKa = 10.34VFF100 pKa = 4.47

Molecular weight: 11.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A185AMW6|A0A185AMW6_9CAUD Holin OS=Staphylococcus phage BP39 OX=1543206 GN=BP39_11 PE=4 SV=1
MM1 pKa = 7.66NDD3 pKa = 2.99QEE5 pKa = 5.77KK6 pKa = 10.06IDD8 pKa = 4.58KK9 pKa = 7.93FTHH12 pKa = 6.46SYY14 pKa = 10.93INDD17 pKa = 3.77DD18 pKa = 3.78FGLTIDD24 pKa = 3.66QLVPKK29 pKa = 9.85VKK31 pKa = 10.36GYY33 pKa = 11.2GRR35 pKa = 11.84FNVWLGGNEE44 pKa = 4.07SKK46 pKa = 10.39IRR48 pKa = 11.84QVLKK52 pKa = 9.89AVKK55 pKa = 9.61EE56 pKa = 4.15IGVSPTLFAVYY67 pKa = 9.33EE68 pKa = 4.19KK69 pKa = 11.33NEE71 pKa = 4.39GYY73 pKa = 10.08SAGLGWLNHH82 pKa = 4.64TSARR86 pKa = 11.84GDD88 pKa = 3.67YY89 pKa = 9.83LTDD92 pKa = 3.0AKK94 pKa = 10.81FIARR98 pKa = 11.84KK99 pKa = 9.11LVSQSKK105 pKa = 9.27QAGQPSWYY113 pKa = 9.92DD114 pKa = 3.2YY115 pKa = 11.1GNPVHH120 pKa = 7.0FVPQDD125 pKa = 3.43VQRR128 pKa = 11.84KK129 pKa = 8.58GNADD133 pKa = 3.15FSKK136 pKa = 10.88NMKK139 pKa = 10.18AGTIGRR145 pKa = 11.84AYY147 pKa = 9.86IPLTAAATWAAYY159 pKa = 10.19YY160 pKa = 9.97PLGLKK165 pKa = 10.27ASYY168 pKa = 11.1NKK170 pKa = 8.87VQNYY174 pKa = 8.36GNPFLDD180 pKa = 4.01GANTILAWGGKK191 pKa = 8.9IDD193 pKa = 4.74GKK195 pKa = 10.89GGSPNSGSSDD205 pKa = 3.32SSSDD209 pKa = 3.31SGGNSLLALAKK220 pKa = 10.19QAMQEE225 pKa = 4.1LLKK228 pKa = 10.63KK229 pKa = 10.48VQDD232 pKa = 3.53ALQWDD237 pKa = 4.06VHH239 pKa = 7.39SIGSDD244 pKa = 2.68KK245 pKa = 10.79FFSNDD250 pKa = 2.89YY251 pKa = 8.37FTLQKK256 pKa = 9.76TFNNTYY262 pKa = 9.12HH263 pKa = 7.0IKK265 pKa = 8.04MTIGLLDD272 pKa = 3.83SLKK275 pKa = 10.93KK276 pKa = 10.89LIDD279 pKa = 3.73SVQVDD284 pKa = 3.66SGGSSSNPTDD294 pKa = 4.41DD295 pKa = 6.01DD296 pKa = 4.66GDD298 pKa = 3.93HH299 pKa = 6.69KK300 pKa = 10.7PISGKK305 pKa = 7.43SVKK308 pKa = 10.22PNGKK312 pKa = 8.68SGRR315 pKa = 11.84VIGGNWTYY323 pKa = 11.51AQLPEE328 pKa = 4.51KK329 pKa = 9.49YY330 pKa = 9.06KK331 pKa = 10.77KK332 pKa = 10.74AIGVPLFKK340 pKa = 10.35KK341 pKa = 10.27EE342 pKa = 3.9YY343 pKa = 9.69LYY345 pKa = 11.13KK346 pKa = 10.3PGNIFPQTGNAGQCTEE362 pKa = 4.3LTWAYY367 pKa = 9.46MSQLHH372 pKa = 6.84GKK374 pKa = 8.57RR375 pKa = 11.84QPTDD379 pKa = 3.37DD380 pKa = 3.88GQVTNGQRR388 pKa = 11.84VWYY391 pKa = 9.59VYY393 pKa = 10.68KK394 pKa = 10.88KK395 pKa = 10.51LGAKK399 pKa = 6.42TTHH402 pKa = 6.34NPTVGYY408 pKa = 9.76GFSSKK413 pKa = 10.35PPYY416 pKa = 10.08LQATAYY422 pKa = 10.12GIGHH426 pKa = 6.67TGVVVAVFDD435 pKa = 4.62DD436 pKa = 4.68GSFLVANYY444 pKa = 8.94NVPPYY449 pKa = 9.33VAPSRR454 pKa = 11.84VVLYY458 pKa = 8.72TLINGVPHH466 pKa = 6.57NAGDD470 pKa = 3.9NIVFFSGIAA479 pKa = 3.09

Molecular weight: 52.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

5467

54

762

273.4

31.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.951 ± 0.42

0.512 ± 0.147

7.481 ± 0.354

6.292 ± 0.677

5.433 ± 0.389

4.701 ± 0.881

1.774 ± 0.222

7.353 ± 0.594

8.579 ± 0.364

7.92 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.292

8.652 ± 0.545

2.543 ± 0.325

3.933 ± 0.353

3.329 ± 0.294

6.311 ± 0.361

6.109 ± 0.426

5.396 ± 0.302

0.933 ± 0.222

6.219 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski