Peptoniphilus lacrimalis 315-B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; Peptoniphilus lacrimalis

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1VTC7|D1VTC7_9FIRM Acyl-ACP thioesterase OS=Peptoniphilus lacrimalis 315-B OX=596330 GN=HMPREF0628_0388 PE=4 SV=1
MM1 pKa = 7.89AEE3 pKa = 3.93NEE5 pKa = 4.4KK6 pKa = 10.96NCGCSCGCSHH16 pKa = 7.35EE17 pKa = 4.75EE18 pKa = 4.02GHH20 pKa = 6.16QEE22 pKa = 4.17GYY24 pKa = 10.98DD25 pKa = 3.77IINLSLDD32 pKa = 3.33DD33 pKa = 4.62DD34 pKa = 4.78RR35 pKa = 11.84EE36 pKa = 3.84VSYY39 pKa = 10.75IILGIFEE46 pKa = 5.25CDD48 pKa = 3.34DD49 pKa = 3.59EE50 pKa = 5.41EE51 pKa = 5.19YY52 pKa = 10.51IALIPEE58 pKa = 4.37DD59 pKa = 4.03EE60 pKa = 4.56KK61 pKa = 11.44EE62 pKa = 4.43GEE64 pKa = 4.01DD65 pKa = 4.04VYY67 pKa = 11.4LFRR70 pKa = 11.84YY71 pKa = 9.4KK72 pKa = 10.63EE73 pKa = 3.99SGEE76 pKa = 4.36DD77 pKa = 3.56EE78 pKa = 4.53VEE80 pKa = 3.66LDD82 pKa = 3.7EE83 pKa = 5.84IEE85 pKa = 4.42NEE87 pKa = 4.16EE88 pKa = 4.07EE89 pKa = 3.71FDD91 pKa = 3.92RR92 pKa = 11.84VSKK95 pKa = 11.02KK96 pKa = 10.12FDD98 pKa = 3.16QLFIEE103 pKa = 4.44EE104 pKa = 4.9DD105 pKa = 3.75DD106 pKa = 4.17FNEE109 pKa = 4.07

Molecular weight: 12.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1VRL9|D1VRL9_9FIRM Peptidase M16 inactive domain protein OS=Peptoniphilus lacrimalis 315-B OX=596330 GN=HMPREF0628_1616 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.18NKK10 pKa = 8.76QRR12 pKa = 11.84KK13 pKa = 7.85RR14 pKa = 11.84EE15 pKa = 3.9HH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84NVIKK32 pKa = 10.48ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.94GRR39 pKa = 11.84KK40 pKa = 8.89RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1588

0

1588

511444

37

4631

322.1

36.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.441 ± 0.063

0.935 ± 0.024

6.316 ± 0.065

7.226 ± 0.07

4.793 ± 0.056

6.127 ± 0.055

1.238 ± 0.023

9.543 ± 0.082

9.98 ± 0.072

9.34 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.033

6.31 ± 0.057

2.782 ± 0.047

2.343 ± 0.027

3.536 ± 0.044

6.386 ± 0.049

4.586 ± 0.068

5.912 ± 0.054

0.568 ± 0.021

4.288 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski