Amino acid dipepetide frequency for Bacillus sp. Y1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.29AlaAla: 5.29 ± 0.09	0.573AlaCys: 0.573 ± 0.023	3.095AlaAsp: 3.095 ± 0.049	4.404AlaGlu: 4.404 ± 0.06	3.244AlaPhe: 3.244 ± 0.045	5.038AlaGly: 5.038 ± 0.071	1.233AlaHis: 1.233 ± 0.032	5.615AlaIle: 5.615 ± 0.075	4.475AlaLys: 4.475 ± 0.068	7.021AlaLeu: 7.021 ± 0.073	1.889AlaMet: 1.889 ± 0.038	2.848AlaAsn: 2.848 ± 0.048	1.975AlaPro: 1.975 ± 0.046	2.017AlaGln: 2.017 ± 0.039	2.485AlaArg: 2.485 ± 0.047	4.072AlaSer: 4.072 ± 0.06	3.572AlaThr: 3.572 ± 0.054	5.103AlaVal: 5.103 ± 0.072	0.628AlaTrp: 0.628 ± 0.022	2.159AlaTyr: 2.159 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.396CysAla: 0.396 ± 0.019	0.084CysCys: 0.084 ± 0.008	0.401CysAsp: 0.401 ± 0.018	0.484CysGlu: 0.484 ± 0.02	0.336CysPhe: 0.336 ± 0.017	0.65CysGly: 0.65 ± 0.025	0.218CysHis: 0.218 ± 0.014	0.516CysIle: 0.516 ± 0.021	0.4CysLys: 0.4 ± 0.018	0.682CysLeu: 0.682 ± 0.023	0.175CysMet: 0.175 ± 0.012	0.281CysAsn: 0.281 ± 0.015	0.344CysPro: 0.344 ± 0.017	0.222CysGln: 0.222 ± 0.013	0.278CysArg: 0.278 ± 0.015	0.539CysSer: 0.539 ± 0.022	0.392CysThr: 0.392 ± 0.018	0.42CysVal: 0.42 ± 0.02	0.06CysTrp: 0.06 ± 0.006	0.26CysTyr: 0.26 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.052AspAla: 3.052 ± 0.05	0.372AspCys: 0.372 ± 0.018	2.352AspAsp: 2.352 ± 0.044	4.041AspGlu: 4.041 ± 0.062	2.374AspPhe: 2.374 ± 0.04	3.359AspGly: 3.359 ± 0.052	1.196AspHis: 1.196 ± 0.03	4.06AspIle: 4.06 ± 0.058	2.8AspLys: 2.8 ± 0.043	5.022AspLeu: 5.022 ± 0.054	1.297AspMet: 1.297 ± 0.03	1.715AspAsn: 1.715 ± 0.042	1.96AspPro: 1.96 ± 0.044	2.033AspGln: 2.033 ± 0.041	2.253AspArg: 2.253 ± 0.041	2.708AspSer: 2.708 ± 0.043	2.376AspThr: 2.376 ± 0.041	3.739AspVal: 3.739 ± 0.049	0.652AspTrp: 0.652 ± 0.023	2.139AspTyr: 2.139 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	5.246GluAla: 5.246 ± 0.071	0.381GluCys: 0.381 ± 0.017	3.682GluAsp: 3.682 ± 0.062	7.384GluGlu: 7.384 ± 0.098	2.72GluPhe: 2.72 ± 0.047	4.528GluGly: 4.528 ± 0.058	1.508GluHis: 1.508 ± 0.034	5.715GluIle: 5.715 ± 0.082	6.699GluLys: 6.699 ± 0.077	7.452GluLeu: 7.452 ± 0.072	2.268GluMet: 2.268 ± 0.041	3.715GluAsn: 3.715 ± 0.054	1.889GluPro: 1.889 ± 0.034	3.161GluGln: 3.161 ± 0.058	3.413GluArg: 3.413 ± 0.055	3.726GluSer: 3.726 ± 0.053	3.895GluThr: 3.895 ± 0.054	5.41GluVal: 5.41 ± 0.065	0.818GluTrp: 0.818 ± 0.029	2.406GluTyr: 2.406 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	2.947PheAla: 2.947 ± 0.049	0.332PheCys: 0.332 ± 0.015	2.328PheAsp: 2.328 ± 0.037	2.914PheGlu: 2.914 ± 0.045	2.433PhePhe: 2.433 ± 0.053	3.427PheGly: 3.427 ± 0.058	1.08PheHis: 1.08 ± 0.026	3.974PheIle: 3.974 ± 0.055	2.36PheLys: 2.36 ± 0.042	4.729PheLeu: 4.729 ± 0.071	1.171PheMet: 1.171 ± 0.031	1.829PheAsn: 1.829 ± 0.041	1.731PhePro: 1.731 ± 0.038	1.722PheGln: 1.722 ± 0.034	1.546PheArg: 1.546 ± 0.036	3.464PheSer: 3.464 ± 0.05	2.646PheThr: 2.646 ± 0.045	3.261PheVal: 3.261 ± 0.051	0.48PheTrp: 0.48 ± 0.02	1.772PheTyr: 1.772 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.751GlyAla: 4.751 ± 0.07	0.642GlyCys: 0.642 ± 0.025	3.15GlyAsp: 3.15 ± 0.049	4.742GlyGlu: 4.742 ± 0.069	3.523GlyPhe: 3.523 ± 0.059	4.863GlyGly: 4.863 ± 0.078	1.358GlyHis: 1.358 ± 0.036	5.907GlyIle: 5.907 ± 0.07	4.993GlyLys: 4.993 ± 0.066	6.645GlyLeu: 6.645 ± 0.067	2.14GlyMet: 2.14 ± 0.039	2.855GlyAsn: 2.855 ± 0.054	1.749GlyPro: 1.749 ± 0.039	2.108GlyGln: 2.108 ± 0.047	2.59GlyArg: 2.59 ± 0.055	4.032GlySer: 4.032 ± 0.056	4.124GlyThr: 4.124 ± 0.059	5.275GlyVal: 5.275 ± 0.066	0.841GlyTrp: 0.841 ± 0.028	2.796GlyTyr: 2.796 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.257HisAla: 1.257 ± 0.029	0.187HisCys: 0.187 ± 0.013	0.996HisAsp: 0.996 ± 0.023	1.443HisGlu: 1.443 ± 0.032	1.15HisPhe: 1.15 ± 0.032	1.406HisGly: 1.406 ± 0.034	0.648HisHis: 0.648 ± 0.025	1.55HisIle: 1.55 ± 0.032	1.024HisLys: 1.024 ± 0.029	2.092HisLeu: 2.092 ± 0.049	0.511HisMet: 0.511 ± 0.02	0.767HisAsn: 0.767 ± 0.025	1.122HisPro: 1.122 ± 0.03	0.846HisGln: 0.846 ± 0.029	0.832HisArg: 0.832 ± 0.024	1.34HisSer: 1.34 ± 0.031	1.062HisThr: 1.062 ± 0.027	1.455HisVal: 1.455 ± 0.035	0.232HisTrp: 0.232 ± 0.014	0.848HisTyr: 0.848 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.662IleAla: 5.662 ± 0.078	0.666IleCys: 0.666 ± 0.024	4.193IleAsp: 4.193 ± 0.059	5.809IleGlu: 5.809 ± 0.071	3.315IlePhe: 3.315 ± 0.056	6.005IleGly: 6.005 ± 0.073	1.81IleHis: 1.81 ± 0.038	5.967IleIle: 5.967 ± 0.082	4.663IleLys: 4.663 ± 0.056	7.343IleLeu: 7.343 ± 0.088	1.803IleMet: 1.803 ± 0.035	3.358IleAsn: 3.358 ± 0.062	3.418IlePro: 3.418 ± 0.056	3.081IleGln: 3.081 ± 0.043	2.935IleArg: 2.935 ± 0.052	5.412IleSer: 5.412 ± 0.074	4.545IleThr: 4.545 ± 0.058	5.723IleVal: 5.723 ± 0.07	0.67IleTrp: 0.67 ± 0.024	2.486IleTyr: 2.486 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.413LysAla: 4.413 ± 0.068	0.327LysCys: 0.327 ± 0.017	3.714LysAsp: 3.714 ± 0.058	7.044LysGlu: 7.044 ± 0.073	1.967LysPhe: 1.967 ± 0.042	4.584LysGly: 4.584 ± 0.058	1.313LysHis: 1.313 ± 0.034	4.703LysIle: 4.703 ± 0.067	5.736LysLys: 5.736 ± 0.074	5.932LysLeu: 5.932 ± 0.073	2.177LysMet: 2.177 ± 0.036	3.325LysAsn: 3.325 ± 0.05	2.125LysPro: 2.125 ± 0.035	2.906LysGln: 2.906 ± 0.049	3.036LysArg: 3.036 ± 0.043	3.446LysSer: 3.446 ± 0.061	3.418LysThr: 3.418 ± 0.05	4.977LysVal: 4.977 ± 0.068	0.797LysTrp: 0.797 ± 0.025	2.2LysTyr: 2.2 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	7.115LeuAla: 7.115 ± 0.081	0.68LeuCys: 0.68 ± 0.019	4.702LeuAsp: 4.702 ± 0.068	6.832LeuGlu: 6.832 ± 0.083	4.97LeuPhe: 4.97 ± 0.081	6.458LeuGly: 6.458 ± 0.079	1.994LeuHis: 1.994 ± 0.042	7.197LeuIle: 7.197 ± 0.079	6.497LeuLys: 6.497 ± 0.064	10.54LeuLeu: 10.54 ± 0.113	2.441LeuMet: 2.441 ± 0.046	4.138LeuAsn: 4.138 ± 0.062	3.932LeuPro: 3.932 ± 0.057	3.829LeuGln: 3.829 ± 0.05	3.557LeuArg: 3.557 ± 0.056	7.241LeuSer: 7.241 ± 0.074	5.865LeuThr: 5.865 ± 0.072	6.614LeuVal: 6.614 ± 0.071	0.835LeuTrp: 0.835 ± 0.026	3.247LeuTyr: 3.247 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	1.926MetAla: 1.926 ± 0.041	0.14MetCys: 0.14 ± 0.011	1.4MetAsp: 1.4 ± 0.033	1.988MetGlu: 1.988 ± 0.034	1.12MetPhe: 1.12 ± 0.029	1.763MetGly: 1.763 ± 0.032	0.383MetHis: 0.383 ± 0.016	2.223MetIle: 2.223 ± 0.044	2.475MetLys: 2.475 ± 0.04	2.533MetLeu: 2.533 ± 0.047	0.841MetMet: 0.841 ± 0.025	1.597MetAsn: 1.597 ± 0.029	0.983MetPro: 0.983 ± 0.034	0.784MetGln: 0.784 ± 0.026	1.019MetArg: 1.019 ± 0.028	1.636MetSer: 1.636 ± 0.033	1.59MetThr: 1.59 ± 0.035	1.891MetVal: 1.891 ± 0.039	0.175MetTrp: 0.175 ± 0.011	0.801MetTyr: 0.801 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.517AsnAla: 2.517 ± 0.047	0.332AsnCys: 0.332 ± 0.018	2.211AsnAsp: 2.211 ± 0.044	3.657AsnGlu: 3.657 ± 0.053	1.643AsnPhe: 1.643 ± 0.038	3.516AsnGly: 3.516 ± 0.061	1.107AsnHis: 1.107 ± 0.028	3.415AsnIle: 3.415 ± 0.057	2.899AsnLys: 2.899 ± 0.048	3.873AsnLeu: 3.873 ± 0.053	1.22AsnMet: 1.22 ± 0.029	2.024AsnAsn: 2.024 ± 0.047	2.113AsnPro: 2.113 ± 0.049	1.992AsnGln: 1.992 ± 0.044	1.981AsnArg: 1.981 ± 0.04	2.516AsnSer: 2.516 ± 0.044	2.234AsnThr: 2.234 ± 0.04	2.963AsnVal: 2.963 ± 0.047	0.578AsnTrp: 0.578 ± 0.023	1.65AsnTyr: 1.65 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	2.123ProAla: 2.123 ± 0.039	0.213ProCys: 0.213 ± 0.013	1.93ProAsp: 1.93 ± 0.047	2.833ProGlu: 2.833 ± 0.051	2.101ProPhe: 2.101 ± 0.042	2.261ProGly: 2.261 ± 0.046	0.764ProHis: 0.764 ± 0.025	2.994ProIle: 2.994 ± 0.043	2.102ProLys: 2.102 ± 0.04	3.509ProLeu: 3.509 ± 0.05	0.833ProMet: 0.833 ± 0.027	1.776ProAsn: 1.776 ± 0.038	0.948ProPro: 0.948 ± 0.026	1.07ProGln: 1.07 ± 0.026	1.06ProArg: 1.06 ± 0.027	2.343ProSer: 2.343 ± 0.046	2.088ProThr: 2.088 ± 0.043	2.793ProVal: 2.793 ± 0.047	0.41ProTrp: 0.41 ± 0.017	1.394ProTyr: 1.394 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.608GlnAla: 2.608 ± 0.046	0.191GlnCys: 0.191 ± 0.012	1.494GlnAsp: 1.494 ± 0.031	2.751GlnGlu: 2.751 ± 0.048	1.624GlnPhe: 1.624 ± 0.036	2.122GlnGly: 2.122 ± 0.039	0.706GlnHis: 0.706 ± 0.022	2.674GlnIle: 2.674 ± 0.043	2.637GlnLys: 2.637 ± 0.04	4.056GlnLeu: 4.056 ± 0.062	1.137GlnMet: 1.137 ± 0.03	1.57GlnAsn: 1.57 ± 0.037	1.22GlnPro: 1.22 ± 0.032	1.672GlnGln: 1.672 ± 0.048	1.395GlnArg: 1.395 ± 0.029	2.276GlnSer: 2.276 ± 0.04	2.072GlnThr: 2.072 ± 0.041	2.554GlnVal: 2.554 ± 0.044	0.405GlnTrp: 0.405 ± 0.017	1.36GlnTyr: 1.36 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.335ArgAla: 2.335 ± 0.044	0.244ArgCys: 0.244 ± 0.014	2.003ArgAsp: 2.003 ± 0.04	3.239ArgGlu: 3.239 ± 0.051	1.844ArgPhe: 1.844 ± 0.047	2.278ArgGly: 2.278 ± 0.039	0.716ArgHis: 0.716 ± 0.024	2.896ArgIle: 2.896 ± 0.052	3.063ArgLys: 3.063 ± 0.045	3.846ArgLeu: 3.846 ± 0.058	1.278ArgMet: 1.278 ± 0.031	1.902ArgAsn: 1.902 ± 0.03	1.247ArgPro: 1.247 ± 0.034	1.325ArgGln: 1.325 ± 0.035	1.656ArgArg: 1.656 ± 0.036	2.173ArgSer: 2.173 ± 0.042	2.011ArgThr: 2.011 ± 0.034	2.681ArgVal: 2.681 ± 0.044	0.444ArgTrp: 0.444 ± 0.019	1.517ArgTyr: 1.517 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	3.678SerAla: 3.678 ± 0.053	0.437SerCys: 0.437 ± 0.019	2.909SerAsp: 2.909 ± 0.047	4.191SerGlu: 4.191 ± 0.055	3.446SerPhe: 3.446 ± 0.05	4.465SerGly: 4.465 ± 0.061	1.25SerHis: 1.25 ± 0.031	5.38SerIle: 5.38 ± 0.069	4.17SerLys: 4.17 ± 0.045	6.394SerLeu: 6.394 ± 0.068	1.739SerMet: 1.739 ± 0.038	2.852SerAsn: 2.852 ± 0.053	2.166SerPro: 2.166 ± 0.038	2.062SerGln: 2.062 ± 0.04	2.222SerArg: 2.222 ± 0.039	4.328SerSer: 4.328 ± 0.058	3.511SerThr: 3.511 ± 0.055	4.411SerVal: 4.411 ± 0.062	0.683SerTrp: 0.683 ± 0.027	2.47SerTyr: 2.47 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.815ThrAla: 3.815 ± 0.063	0.355ThrCys: 0.355 ± 0.015	2.733ThrAsp: 2.733 ± 0.041	3.635ThrGlu: 3.635 ± 0.05	2.745ThrPhe: 2.745 ± 0.048	4.177ThrGly: 4.177 ± 0.055	1.057ThrHis: 1.057 ± 0.028	4.816ThrIle: 4.816 ± 0.065	3.391ThrLys: 3.391 ± 0.052	5.405ThrLeu: 5.405 ± 0.067	1.317ThrMet: 1.317 ± 0.033	2.613ThrAsn: 2.613 ± 0.046	2.283ThrPro: 2.283 ± 0.041	1.516ThrGln: 1.516 ± 0.034	1.827ThrArg: 1.827 ± 0.032	3.623ThrSer: 3.623 ± 0.051	3.197ThrThr: 3.197 ± 0.057	4.225ThrVal: 4.225 ± 0.064	0.581ThrTrp: 0.581 ± 0.025	2.069ThrTyr: 2.069 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	5.025ValAla: 5.025 ± 0.071	0.588ValCys: 0.588 ± 0.024	3.775ValAsp: 3.775 ± 0.051	5.166ValGlu: 5.166 ± 0.068	3.234ValPhe: 3.234 ± 0.046	4.898ValGly: 4.898 ± 0.064	1.337ValHis: 1.337 ± 0.034	5.861ValIle: 5.861 ± 0.073	4.853ValLys: 4.853 ± 0.062	6.981ValLeu: 6.981 ± 0.071	1.807ValMet: 1.807 ± 0.039	3.21ValAsn: 3.21 ± 0.05	2.683ValPro: 2.683 ± 0.04	2.341ValGln: 2.341 ± 0.037	2.621ValArg: 2.621 ± 0.044	4.844ValSer: 4.844 ± 0.063	4.34ValThr: 4.34 ± 0.062	5.39ValVal: 5.39 ± 0.078	0.68ValTrp: 0.68 ± 0.022	2.365ValTyr: 2.365 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.633TrpAla: 0.633 ± 0.024	0.077TrpCys: 0.077 ± 0.007	0.522TrpAsp: 0.522 ± 0.023	0.716TrpGlu: 0.716 ± 0.023	0.485TrpPhe: 0.485 ± 0.021	0.743TrpGly: 0.743 ± 0.029	0.192TrpHis: 0.192 ± 0.014	0.821TrpIle: 0.821 ± 0.027	0.74TrpLys: 0.74 ± 0.023	1.126TrpLeu: 1.126 ± 0.029	0.371TrpMet: 0.371 ± 0.017	0.579TrpAsn: 0.579 ± 0.02	0.256TrpPro: 0.256 ± 0.016	0.351TrpGln: 0.351 ± 0.015	0.399TrpArg: 0.399 ± 0.019	0.686TrpSer: 0.686 ± 0.024	0.574TrpThr: 0.574 ± 0.018	0.735TrpVal: 0.735 ± 0.022	0.144TrpTrp: 0.144 ± 0.01	0.371TrpTyr: 0.371 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.949TyrAla: 1.949 ± 0.041	0.319TyrCys: 0.319 ± 0.016	1.912TyrAsp: 1.912 ± 0.038	2.747TyrGlu: 2.747 ± 0.046	1.869TyrPhe: 1.869 ± 0.04	2.541TyrGly: 2.541 ± 0.044	0.897TyrHis: 0.897 ± 0.027	2.581TyrIle: 2.581 ± 0.05	2.163TyrLys: 2.163 ± 0.04	3.506TyrLeu: 3.506 ± 0.058	0.854TyrMet: 0.854 ± 0.024	1.487TyrAsn: 1.487 ± 0.036	1.401TyrPro: 1.401 ± 0.036	1.482TyrGln: 1.482 ± 0.036	1.592TyrArg: 1.592 ± 0.036	2.358TyrSer: 2.358 ± 0.046	1.871TyrThr: 1.871 ± 0.041	2.38TyrVal: 2.38 ± 0.036	0.405TyrTrp: 0.405 ± 0.018	1.472TyrTyr: 1.472 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4676 proteins (1408612 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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