Alkalihalobacillus nanhaiisediminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3479 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562QSY2|A0A562QSY2_9BACI Probable lipid II flippase MurJ OS=Alkalihalobacillus nanhaiisediminis OX=688079 GN=murJ PE=3 SV=1
MM1 pKa = 7.23KK2 pKa = 10.26KK3 pKa = 9.84WMISLGLALVLTACGGSEE21 pKa = 4.13TEE23 pKa = 4.57EE24 pKa = 4.21TTEE27 pKa = 4.12TPAEE31 pKa = 4.18GSEE34 pKa = 4.1EE35 pKa = 3.92AVEE38 pKa = 4.16EE39 pKa = 4.73GSDD42 pKa = 3.65EE43 pKa = 4.15VFEE46 pKa = 4.3IAVIPSQSIGEE57 pKa = 4.32MEE59 pKa = 3.97TGLNNLEE66 pKa = 3.77EE67 pKa = 4.37HH68 pKa = 6.36LADD71 pKa = 3.68RR72 pKa = 11.84LGRR75 pKa = 11.84EE76 pKa = 4.42VVVEE80 pKa = 3.96QYY82 pKa = 10.93PNYY85 pKa = 9.52NAVVEE90 pKa = 4.19ALNYY94 pKa = 10.19NHH96 pKa = 7.49IDD98 pKa = 3.57LAYY101 pKa = 10.28LGPLTYY107 pKa = 10.64LIAHH111 pKa = 6.27EE112 pKa = 4.08QSGAKK117 pKa = 10.14AIITQEE123 pKa = 3.84IDD125 pKa = 3.04GSPYY129 pKa = 10.01YY130 pKa = 10.3YY131 pKa = 11.14SNIITHH137 pKa = 7.37ADD139 pKa = 3.58AEE141 pKa = 4.49WEE143 pKa = 4.04NLEE146 pKa = 5.83DD147 pKa = 3.75MLEE150 pKa = 4.24DD151 pKa = 3.43RR152 pKa = 11.84AEE154 pKa = 3.87VDD156 pKa = 3.97FAFASISSTSGHH168 pKa = 7.68LIPGLEE174 pKa = 3.97LRR176 pKa = 11.84NLGYY180 pKa = 10.57YY181 pKa = 9.9EE182 pKa = 5.37SEE184 pKa = 4.55DD185 pKa = 3.17NHH187 pKa = 6.41EE188 pKa = 4.27FNQIQFAGSHH198 pKa = 6.2DD199 pKa = 4.0VVATLVQDD207 pKa = 3.53QTVDD211 pKa = 2.92AGAIDD216 pKa = 3.99SAILEE221 pKa = 4.22ALMTEE226 pKa = 4.52DD227 pKa = 4.13EE228 pKa = 4.68NNGGTLRR235 pKa = 11.84DD236 pKa = 4.4DD237 pKa = 4.73IKK239 pKa = 11.36VIWQSEE245 pKa = 3.94QLYY248 pKa = 9.13QYY250 pKa = 9.25PWVVPAQMDD259 pKa = 3.88DD260 pKa = 3.55EE261 pKa = 5.78LISQVQEE268 pKa = 3.53AFYY271 pKa = 10.38EE272 pKa = 4.26IEE274 pKa = 4.29DD275 pKa = 3.97EE276 pKa = 4.49EE277 pKa = 4.22ILRR280 pKa = 11.84IFGGATKK287 pKa = 10.1FVEE290 pKa = 4.91ADD292 pKa = 3.22DD293 pKa = 4.06SQYY296 pKa = 11.89ADD298 pKa = 3.24VLEE301 pKa = 4.44AARR304 pKa = 11.84EE305 pKa = 3.83FDD307 pKa = 3.46MLTLEE312 pKa = 4.5EE313 pKa = 4.23EE314 pKa = 4.36

Molecular weight: 35.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562QSG2|A0A562QSG2_9BACI Uncharacterized protein OS=Alkalihalobacillus nanhaiisediminis OX=688079 GN=IQ10_00832 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.15KK15 pKa = 9.35KK16 pKa = 9.06HH17 pKa = 4.22GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.22NGRR29 pKa = 11.84QVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3479

0

3479

1004815

16

1686

288.8

32.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.239 ± 0.045

0.7 ± 0.011

5.053 ± 0.03

7.895 ± 0.051

4.36 ± 0.032

6.866 ± 0.041

2.213 ± 0.022

7.7 ± 0.038

6.303 ± 0.038

9.821 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.019

4.057 ± 0.03

3.601 ± 0.023

4.048 ± 0.033

4.366 ± 0.028

5.843 ± 0.031

5.51 ± 0.027

7.315 ± 0.032

0.967 ± 0.016

3.295 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski