Gordonia phage Nedarya

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Soupsvirus; unclassified Soupsvirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q1L1|A0A2Z4Q1L1_9CAUD DsDNA partitioning protein OS=Gordonia phage Nedarya OX=2234025 GN=35 PE=4 SV=1
MM1 pKa = 8.49SEE3 pKa = 4.37MDD5 pKa = 4.01NPLNDD10 pKa = 3.63LMSFGNVVYY19 pKa = 10.74SDD21 pKa = 4.39GEE23 pKa = 4.25GSVTDD28 pKa = 4.18EE29 pKa = 4.86FDD31 pKa = 3.47DD32 pKa = 4.14TVFGPEE38 pKa = 3.9VVYY41 pKa = 11.27VDD43 pKa = 4.37LNEE46 pKa = 4.43EE47 pKa = 4.48GSVSSPEE54 pKa = 4.07TIEE57 pKa = 3.68MSGYY61 pKa = 10.76GDD63 pKa = 3.48WEE65 pKa = 4.17LLQGFTGQYY74 pKa = 9.94SYY76 pKa = 11.28NGPIMHH82 pKa = 7.4DD83 pKa = 3.27SEE85 pKa = 5.16YY86 pKa = 10.53IGGRR90 pKa = 11.84LEE92 pKa = 3.79DD93 pKa = 4.39HH94 pKa = 6.22IRR96 pKa = 11.84EE97 pKa = 4.03NRR99 pKa = 11.84GYY101 pKa = 8.28YY102 pKa = 9.99AAVIVDD108 pKa = 3.8GDD110 pKa = 3.73RR111 pKa = 11.84TDD113 pKa = 4.48EE114 pKa = 4.14NDD116 pKa = 2.87EE117 pKa = 4.52SIAVGWAVAFKK128 pKa = 11.12KK129 pKa = 10.49EE130 pKa = 3.93VV131 pKa = 3.03

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q1A9|A0A2Z4Q1A9_9CAUD Uncharacterized protein OS=Gordonia phage Nedarya OX=2234025 GN=61 PE=4 SV=1
MM1 pKa = 7.25SWAGSSRR8 pKa = 11.84RR9 pKa = 11.84QRR11 pKa = 11.84LPKK14 pKa = 10.3DD15 pKa = 2.91WEE17 pKa = 4.08LKK19 pKa = 9.73YY20 pKa = 10.47RR21 pKa = 11.84LPVLSRR27 pKa = 11.84DD28 pKa = 3.11RR29 pKa = 11.84WLCQINGPGCKK40 pKa = 9.08RR41 pKa = 11.84AARR44 pKa = 11.84DD45 pKa = 2.95VDD47 pKa = 4.41HH48 pKa = 7.25IVAGDD53 pKa = 3.65NHH55 pKa = 6.65SHH57 pKa = 7.71DD58 pKa = 4.33NLQAVCDD65 pKa = 3.54ACHH68 pKa = 6.49RR69 pKa = 11.84KK70 pKa = 9.78KK71 pKa = 10.91SSAEE75 pKa = 3.96GNSAKK80 pKa = 10.65AKK82 pKa = 10.31LKK84 pKa = 9.33AQRR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84PTEE92 pKa = 3.56RR93 pKa = 11.84HH94 pKa = 5.26PGRR97 pKa = 11.84RR98 pKa = 3.55

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

16332

33

727

168.4

18.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.252 ± 0.276

0.845 ± 0.132

6.539 ± 0.247

6.943 ± 0.306

3.325 ± 0.178

8.346 ± 0.421

1.984 ± 0.176

5.211 ± 0.137

4.458 ± 0.208

8.082 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.149

3.545 ± 0.152

5.009 ± 0.196

3.417 ± 0.171

6.239 ± 0.213

5.866 ± 0.218

6.337 ± 0.32

7.201 ± 0.269

1.892 ± 0.115

3.184 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski