Tortoise microvirus 98

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W706|A0A4P8W706_9VIRU Uncharacterized protein OS=Tortoise microvirus 98 OX=2583207 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.5SFMFNIDD9 pKa = 3.78LQNGSIIDD17 pKa = 4.24SINTPLAAVQLLIPCAMTRR36 pKa = 11.84CIDD39 pKa = 3.62KK40 pKa = 9.26LTEE43 pKa = 3.81RR44 pKa = 11.84ANISLADD51 pKa = 4.73FIRR54 pKa = 11.84LACAEE59 pKa = 4.54YY60 pKa = 10.39ILSFKK65 pKa = 10.79DD66 pKa = 3.18

Molecular weight:
7.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4P8W7Q4|A0A4P8W7Q4_9VIRU DNA pilot protein OS=Tortoise microvirus 98 OX=2583207 PE=4 SV=1
MM1 pKa = 7.26TFSISLQPLNMMRR14 pKa = 11.84WRR16 pKa = 11.84MSDD19 pKa = 3.23MSCSNPLTINVRR31 pKa = 11.84GRR33 pKa = 11.84AVSVPCRR40 pKa = 11.84YY41 pKa = 10.15CMSCRR46 pKa = 11.84TEE48 pKa = 4.02YY49 pKa = 10.73QSALLFGAKK58 pKa = 9.68HH59 pKa = 5.83EE60 pKa = 5.09LFDD63 pKa = 4.85CYY65 pKa = 10.42RR66 pKa = 11.84AGLGASFVTLTYY78 pKa = 10.57SDD80 pKa = 3.54QYY82 pKa = 11.4LPEE85 pKa = 4.8NGSLRR90 pKa = 11.84KK91 pKa = 9.53KK92 pKa = 10.45DD93 pKa = 3.61VQNLLKK99 pKa = 10.51NVRR102 pKa = 11.84IQAKK106 pKa = 9.2RR107 pKa = 11.84KK108 pKa = 7.69TGIPPFKK115 pKa = 10.58YY116 pKa = 10.17IMCGEE121 pKa = 4.19YY122 pKa = 10.4GDD124 pKa = 3.89KK125 pKa = 10.42FGRR128 pKa = 11.84AHH130 pKa = 4.73YY131 pKa = 9.7HH132 pKa = 5.15IVFIGLSDD140 pKa = 3.74VLVHH144 pKa = 6.66NFVKK148 pKa = 10.37PLWKK152 pKa = 10.38FGLIDD157 pKa = 3.52IGALRR162 pKa = 11.84EE163 pKa = 4.12GGLRR167 pKa = 11.84YY168 pKa = 9.55ALKK171 pKa = 10.43YY172 pKa = 7.66CTKK175 pKa = 10.26AARR178 pKa = 11.84GKK180 pKa = 10.15KK181 pKa = 10.14AEE183 pKa = 4.17EE184 pKa = 4.73LYY186 pKa = 11.03DD187 pKa = 3.73NQDD190 pKa = 2.61IEE192 pKa = 5.31RR193 pKa = 11.84PFICHH198 pKa = 5.72SRR200 pKa = 11.84GIGKK204 pKa = 8.56NWFIEE209 pKa = 4.11NADD212 pKa = 4.83DD213 pKa = 5.24IIAHH217 pKa = 6.03NFTYY221 pKa = 10.71LEE223 pKa = 4.03NGIRR227 pKa = 11.84KK228 pKa = 8.25PLPAYY233 pKa = 10.08FRR235 pKa = 11.84RR236 pKa = 11.84QYY238 pKa = 11.51DD239 pKa = 3.23AFKK242 pKa = 10.37TFDD245 pKa = 3.1VSEE248 pKa = 4.62AIKK251 pKa = 10.75YY252 pKa = 10.27LSEE255 pKa = 3.56EE256 pKa = 4.27AKK258 pKa = 9.98IHH260 pKa = 6.75GYY262 pKa = 10.66DD263 pKa = 5.37DD264 pKa = 3.39YY265 pKa = 11.85RR266 pKa = 11.84DD267 pKa = 3.22WSKK270 pKa = 11.28QKK272 pKa = 9.49TYY274 pKa = 7.89QTEE277 pKa = 4.29KK278 pKa = 10.35EE279 pKa = 3.92LTAAARR285 pKa = 11.84ASGIPVDD292 pKa = 4.47DD293 pKa = 3.67SSFRR297 pKa = 11.84LQSGFTANNHH307 pKa = 5.5SPLVNTLLAPRR318 pKa = 11.84RR319 pKa = 11.84EE320 pKa = 3.94ATTNFKK326 pKa = 10.31EE327 pKa = 4.17

Molecular weight:
37.55 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1655

66

583

236.4

26.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.459 ± 0.54

1.269 ± 0.489

6.224 ± 0.222

4.894 ± 0.646

4.592 ± 0.557

5.801 ± 0.588

1.39 ± 0.276

5.74 ± 0.576

4.23 ± 1.04

8.882 ± 0.819

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.142 ± 0.292

5.559 ± 0.589

4.713 ± 0.667

3.565 ± 0.972

6.042 ± 0.291

8.157 ± 0.531

6.405 ± 0.514

5.378 ± 0.735

0.906 ± 0.247

4.653 ± 0.709

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski