Sulfolobus monocaudavirus SMV2

Taxonomy: Viruses; Bicaudaviridae; unclassified Bicaudaviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0N7A9S6|A0A0N7A9S6_9VIRU MoxR-like ATPase OS=Sulfolobus monocaudavirus SMV2 OX=1580591 PE=4 SV=1
MM1 pKa = 7.21SQSQKK6 pKa = 10.61PNLYY10 pKa = 10.38LLSLYY15 pKa = 9.24PSPVGVTVTGDD26 pKa = 3.33TTDD29 pKa = 3.29QTVSFVISPYY39 pKa = 9.75HH40 pKa = 5.69YY41 pKa = 11.04VDD43 pKa = 3.15ISYY46 pKa = 10.73LITSYY51 pKa = 11.63NDD53 pKa = 3.33GVQVYY58 pKa = 9.81APDD61 pKa = 3.86ANIPQSGISGFPPGVPTVTLIIEE84 pKa = 4.34KK85 pKa = 10.73GNLNFEE91 pKa = 4.39FATPEE96 pKa = 4.97DD97 pKa = 3.5ILKK100 pKa = 10.65SYY102 pKa = 10.45LYY104 pKa = 11.24NNILQNMNNDD114 pKa = 3.12LTEE117 pKa = 4.54IYY119 pKa = 10.22QDD121 pKa = 4.81LITLISEE128 pKa = 4.45YY129 pKa = 11.18ASGQSLSQSYY139 pKa = 10.34LSEE142 pKa = 4.14VSSLLQQINNMLQNAEE158 pKa = 4.08QFEE161 pKa = 4.49NTANLSFEE169 pKa = 4.41GVSALQQVYY178 pKa = 11.1NNLNEE183 pKa = 4.42IYY185 pKa = 10.67NSLTNQTLTLGEE197 pKa = 4.55LEE199 pKa = 4.35SLQLPSYY206 pKa = 7.54STPEE210 pKa = 3.76ASSIASNYY218 pKa = 7.33TQFLNNAVSIMQNASSQSGSSSSSSQGSNSSSSTPQSSSSSNASSQSGSSSSPSPLSSLTTNSQSGTSSSSYY290 pKa = 8.96NTQTSTSSTTTITTSTSTSSTTSQTSIISPPTLSQSQVLAQIQAGDD336 pKa = 3.69VSSLLSEE343 pKa = 4.34LSEE346 pKa = 4.45IPSNMIPVVNTVVQLYY362 pKa = 9.36QLYY365 pKa = 9.52GFPDD369 pKa = 4.34GTPLSTVVEE378 pKa = 4.23RR379 pKa = 11.84LLSDD383 pKa = 3.12IRR385 pKa = 11.84EE386 pKa = 3.99AYY388 pKa = 9.85YY389 pKa = 10.86NIVNHH394 pKa = 5.84KK395 pKa = 9.46QEE397 pKa = 4.33EE398 pKa = 4.53VNLQQLSQLLQLYY411 pKa = 7.42NTLVSNGLAPQSQSASKK428 pKa = 10.57LSEE431 pKa = 3.87LTQTSEE437 pKa = 5.04IIPALPPVLGQPYY450 pKa = 9.16PGRR453 pKa = 11.84VNSNSNSNSVNYY465 pKa = 10.84SNFII469 pKa = 3.77

Molecular weight:
50.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0N7AA29|A0A0N7AA29_9VIRU Uncharacterized protein OS=Sulfolobus monocaudavirus SMV2 OX=1580591 PE=4 SV=1
MM1 pKa = 8.29VEE3 pKa = 3.94VKK5 pKa = 10.57LRR7 pKa = 11.84WRR9 pKa = 11.84VKK11 pKa = 9.94INRR14 pKa = 11.84KK15 pKa = 8.62NGKK18 pKa = 9.4EE19 pKa = 3.65YY20 pKa = 9.03MLRR23 pKa = 11.84YY24 pKa = 9.84INVTSRR30 pKa = 11.84SAVFLINYY38 pKa = 8.13VPYY41 pKa = 10.69FNIKK45 pKa = 10.32NNIIEE50 pKa = 4.43FKK52 pKa = 10.14PNRR55 pKa = 11.84RR56 pKa = 11.84YY57 pKa = 10.0AKK59 pKa = 9.15KK60 pKa = 10.12GLRR63 pKa = 11.84IRR65 pKa = 11.84LRR67 pKa = 11.84WLIKK71 pKa = 10.0RR72 pKa = 11.84EE73 pKa = 3.97KK74 pKa = 10.65KK75 pKa = 9.57NGKK78 pKa = 9.14VFTQYY83 pKa = 10.79YY84 pKa = 9.57ISIPSEE90 pKa = 3.61LAKK93 pKa = 10.46PVVDD97 pKa = 4.91LDD99 pKa = 4.14PYY101 pKa = 10.93LVPEE105 pKa = 3.94NMQIVFRR112 pKa = 11.84PKK114 pKa = 10.2EE115 pKa = 3.92GDD117 pKa = 3.54GMGLNNQLFVDD128 pKa = 4.98DD129 pKa = 4.64EE130 pKa = 5.33GEE132 pKa = 4.31TQSQQ136 pKa = 3.19

Molecular weight:
16.23 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

14878

45

1563

228.9

25.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.807 ± 0.382

0.376 ± 0.085

4.315 ± 0.313

6.701 ± 0.672

4.463 ± 0.319

5.175 ± 0.318

0.921 ± 0.09

7.783 ± 0.309

6.466 ± 0.631

10.075 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.191

6.083 ± 0.399

4.241 ± 0.329

4.651 ± 0.657

3.32 ± 0.359

7.494 ± 0.582

5.767 ± 0.46

7.151 ± 0.395

0.86 ± 0.141

5.29 ± 0.363

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski