Synechococcus phage S-H25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tefnutvirus; unclassified Tefnutvirus

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R6Y7|A0A6G8R6Y7_9CAUD Uncharacterized protein OS=Synechococcus phage S-H25 OX=2718941 PE=4 SV=1
MM1 pKa = 7.58SKK3 pKa = 10.53QSLNLGASANDD14 pKa = 3.4NTGDD18 pKa = 3.67TLRR21 pKa = 11.84IGGDD25 pKa = 3.45KK26 pKa = 10.97INDD29 pKa = 3.82NFNEE33 pKa = 4.28IYY35 pKa = 9.17TALGNGVNLTVSLLNPSPGQVLRR58 pKa = 11.84YY59 pKa = 9.05NGSSFVPSNYY69 pKa = 10.29SNLTSALDD77 pKa = 3.57VNGNSIISSSNGNINVATNGTGNLTVGVAGIVSTFSGTTGTIDD120 pKa = 3.25FPTRR124 pKa = 11.84VQYY127 pKa = 11.08KK128 pKa = 10.59NEE130 pKa = 3.97FTSLASAPAAADD142 pKa = 3.43YY143 pKa = 10.67PGFFFTVDD151 pKa = 3.86GDD153 pKa = 3.84DD154 pKa = 3.72KK155 pKa = 11.29PYY157 pKa = 11.31VNINIATGGIGDD169 pKa = 3.77TRR171 pKa = 11.84AEE173 pKa = 4.13VLTEE177 pKa = 3.78YY178 pKa = 11.36ASINTLADD186 pKa = 3.11VDD188 pKa = 4.28VTTTAPTLNQVLKK201 pKa = 10.15WNGSNWVPGDD211 pKa = 3.57DD212 pKa = 3.74VAGVSSINVWEE223 pKa = 4.87SIVSDD228 pKa = 3.95SGTATADD235 pKa = 3.41SQTDD239 pKa = 3.51SLSILGGTNISTTIVDD255 pKa = 3.95DD256 pKa = 4.22ALTIDD261 pKa = 4.07FTGSLTTTLAALTDD275 pKa = 3.85TDD277 pKa = 4.02VSGITQGDD285 pKa = 4.06SLFWDD290 pKa = 3.97GTNWSVTRR298 pKa = 11.84SPRR301 pKa = 11.84IWWEE305 pKa = 3.59LNAVGNEE312 pKa = 4.03HH313 pKa = 5.94YY314 pKa = 9.72TFAGPGFTGTVNDD327 pKa = 3.67PTLYY331 pKa = 9.76VYY333 pKa = 10.65RR334 pKa = 11.84GFTYY338 pKa = 10.62VFDD341 pKa = 4.55NSVQGGGHH349 pKa = 6.7PFRR352 pKa = 11.84IQSTQGLTGTPYY364 pKa = 10.73TDD366 pKa = 3.6GQSGSGSNILYY377 pKa = 6.67WTVPLDD383 pKa = 4.03APSTLYY389 pKa = 10.09YY390 pKa = 10.24ICTLHH395 pKa = 7.07AAMAGTINVVGG406 pKa = 3.81

Molecular weight: 42.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R6Y8|A0A6G8R6Y8_9CAUD Uncharacterized protein OS=Synechococcus phage S-H25 OX=2718941 PE=4 SV=1
MM1 pKa = 7.53WKK3 pKa = 10.23SLTKK7 pKa = 9.64TRR9 pKa = 11.84SAKK12 pKa = 10.25FYY14 pKa = 9.61SNQVKK19 pKa = 9.74FLLLIVVAVLFWNSTDD35 pKa = 2.87ARR37 pKa = 11.84RR38 pKa = 11.84FTGSMLYY45 pKa = 10.43NLGDD49 pKa = 3.36IVQPEE54 pKa = 4.1APQRR58 pKa = 11.84GWDD61 pKa = 3.56YY62 pKa = 11.52RR63 pKa = 3.78

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

213

0

213

54563

36

6384

256.2

28.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.085 ± 0.226

0.863 ± 0.094

6.616 ± 0.144

6.186 ± 0.295

4.292 ± 0.117

7.85 ± 0.347

1.43 ± 0.117

6.268 ± 0.246

5.707 ± 0.467

7.144 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.233

5.848 ± 0.236

3.915 ± 0.155

3.698 ± 0.092

4.052 ± 0.191

7.003 ± 0.229

7.518 ± 0.405

6.788 ± 0.214

1.219 ± 0.088

4.399 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski