Amino acid dipepetide frequency for Caldalkalibacillus thermarum (strain TA2.A1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.948AlaAla: 6.948 ± 0.125	0.824AlaCys: 0.824 ± 0.034	3.707AlaAsp: 3.707 ± 0.078	5.784AlaGlu: 5.784 ± 0.092	3.291AlaPhe: 3.291 ± 0.076	6.303AlaGly: 6.303 ± 0.093	1.809AlaHis: 1.809 ± 0.048	5.359AlaIle: 5.359 ± 0.086	4.496AlaLys: 4.496 ± 0.078	9.069AlaLeu: 9.069 ± 0.124	2.121AlaMet: 2.121 ± 0.05	2.377AlaAsn: 2.377 ± 0.059	2.584AlaPro: 2.584 ± 0.061	3.477AlaGln: 3.477 ± 0.072	4.136AlaArg: 4.136 ± 0.081	3.714AlaSer: 3.714 ± 0.076	3.352AlaThr: 3.352 ± 0.067	6.426AlaVal: 6.426 ± 0.104	0.846AlaTrp: 0.846 ± 0.035	2.831AlaTyr: 2.831 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.546CysAla: 0.546 ± 0.026	0.137CysCys: 0.137 ± 0.015	0.394CysAsp: 0.394 ± 0.019	0.412CysGlu: 0.412 ± 0.025	0.382CysPhe: 0.382 ± 0.023	0.821CysGly: 0.821 ± 0.037	0.288CysHis: 0.288 ± 0.019	0.472CysIle: 0.472 ± 0.025	0.276CysLys: 0.276 ± 0.018	0.891CysLeu: 0.891 ± 0.031	0.184CysMet: 0.184 ± 0.014	0.263CysAsn: 0.263 ± 0.019	0.496CysPro: 0.496 ± 0.028	0.446CysGln: 0.446 ± 0.025	0.508CysArg: 0.508 ± 0.026	0.46CysSer: 0.46 ± 0.022	0.454CysThr: 0.454 ± 0.025	0.535CysVal: 0.535 ± 0.026	0.094CysTrp: 0.094 ± 0.011	0.296CysTyr: 0.296 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.1AspAla: 3.1 ± 0.065	0.382AspCys: 0.382 ± 0.021	2.277AspAsp: 2.277 ± 0.06	4.06AspGlu: 4.06 ± 0.08	1.858AspPhe: 1.858 ± 0.043	3.357AspGly: 3.357 ± 0.073	1.429AspHis: 1.429 ± 0.046	3.563AspIle: 3.563 ± 0.07	2.57AspLys: 2.57 ± 0.063	4.971AspLeu: 4.971 ± 0.085	1.288AspMet: 1.288 ± 0.038	1.383AspAsn: 1.383 ± 0.045	2.692AspPro: 2.692 ± 0.064	2.434AspGln: 2.434 ± 0.055	2.701AspArg: 2.701 ± 0.059	1.808AspSer: 1.808 ± 0.043	2.184AspThr: 2.184 ± 0.049	3.907AspVal: 3.907 ± 0.063	0.671AspTrp: 0.671 ± 0.029	1.84AspTyr: 1.84 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	6.565GluAla: 6.565 ± 0.103	0.357GluCys: 0.357 ± 0.019	3.4GluAsp: 3.4 ± 0.07	7.461GluGlu: 7.461 ± 0.135	1.889GluPhe: 1.889 ± 0.049	4.815GluGly: 4.815 ± 0.092	2.006GluHis: 2.006 ± 0.055	4.885GluIle: 4.885 ± 0.082	5.114GluLys: 5.114 ± 0.103	7.177GluLeu: 7.177 ± 0.111	2.049GluMet: 2.049 ± 0.055	2.436GluAsn: 2.436 ± 0.053	2.36GluPro: 2.36 ± 0.055	4.825GluGln: 4.825 ± 0.101	4.73GluArg: 4.73 ± 0.088	2.716GluSer: 2.716 ± 0.057	3.741GluThr: 3.741 ± 0.072	5.539GluVal: 5.539 ± 0.087	0.98GluTrp: 0.98 ± 0.036	2.02GluTyr: 2.02 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	3.121PheAla: 3.121 ± 0.063	0.387PheCys: 0.387 ± 0.024	2.055PheAsp: 2.055 ± 0.056	2.596PheGlu: 2.596 ± 0.058	1.939PhePhe: 1.939 ± 0.065	2.99PheGly: 2.99 ± 0.073	0.959PheHis: 0.959 ± 0.031	2.983PheIle: 2.983 ± 0.081	2.124PheLys: 2.124 ± 0.052	4.015PheLeu: 4.015 ± 0.085	0.976PheMet: 0.976 ± 0.036	1.478PheAsn: 1.478 ± 0.043	1.668PhePro: 1.668 ± 0.045	1.489PheGln: 1.489 ± 0.043	1.901PheArg: 1.901 ± 0.05	2.393PheSer: 2.393 ± 0.064	2.101PheThr: 2.101 ± 0.05	3.128PheVal: 3.128 ± 0.071	0.456PheTrp: 0.456 ± 0.027	1.481PheTyr: 1.481 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	5.234GlyAla: 5.234 ± 0.093	0.751GlyCys: 0.751 ± 0.034	3.221GlyAsp: 3.221 ± 0.07	5.075GlyGlu: 5.075 ± 0.088	3.091GlyPhe: 3.091 ± 0.071	5.013GlyGly: 5.013 ± 0.096	1.8GlyHis: 1.8 ± 0.047	5.389GlyIle: 5.389 ± 0.094	4.304GlyLys: 4.304 ± 0.083	7.767GlyLeu: 7.767 ± 0.106	2.1GlyMet: 2.1 ± 0.048	2.066GlyAsn: 2.066 ± 0.059	2.196GlyPro: 2.196 ± 0.057	3.387GlyGln: 3.387 ± 0.057	3.84GlyArg: 3.84 ± 0.068	3.523GlySer: 3.523 ± 0.071	3.975GlyThr: 3.975 ± 0.074	5.502GlyVal: 5.502 ± 0.098	0.982GlyTrp: 0.982 ± 0.037	2.829GlyTyr: 2.829 ± 0.061	0.002GlyXaa: 0.002 ± 0.002
His	1.571HisAla: 1.571 ± 0.044	0.27HisCys: 0.27 ± 0.018	1.147HisAsp: 1.147 ± 0.037	1.503HisGlu: 1.503 ± 0.041	1.168HisPhe: 1.168 ± 0.04	1.743HisGly: 1.743 ± 0.047	0.923HisHis: 0.923 ± 0.042	1.784HisIle: 1.784 ± 0.052	1.239HisLys: 1.239 ± 0.04	2.684HisLeu: 2.684 ± 0.057	0.639HisMet: 0.639 ± 0.031	0.712HisAsn: 0.712 ± 0.027	1.667HisPro: 1.667 ± 0.047	1.302HisGln: 1.302 ± 0.04	1.324HisArg: 1.324 ± 0.042	1.131HisSer: 1.131 ± 0.036	1.244HisThr: 1.244 ± 0.04	1.863HisVal: 1.863 ± 0.047	0.3HisTrp: 0.3 ± 0.018	1.081HisTyr: 1.081 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.462IleAla: 5.462 ± 0.086	0.67IleCys: 0.67 ± 0.03	3.682IleAsp: 3.682 ± 0.067	4.896IleGlu: 4.896 ± 0.074	2.483IlePhe: 2.483 ± 0.068	5.332IleGly: 5.332 ± 0.096	1.593IleHis: 1.593 ± 0.049	4.353IleIle: 4.353 ± 0.083	3.695IleLys: 3.695 ± 0.068	6.2IleLeu: 6.2 ± 0.095	1.596IleMet: 1.596 ± 0.046	2.538IleAsn: 2.538 ± 0.058	3.259IlePro: 3.259 ± 0.056	2.473IleGln: 2.473 ± 0.057	3.726IleArg: 3.726 ± 0.07	3.664IleSer: 3.664 ± 0.074	3.564IleThr: 3.564 ± 0.072	5.024IleVal: 5.024 ± 0.085	0.698IleTrp: 0.698 ± 0.03	2.166IleTyr: 2.166 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	4.632LysAla: 4.632 ± 0.084	0.332LysCys: 0.332 ± 0.019	2.664LysAsp: 2.664 ± 0.055	5.448LysGlu: 5.448 ± 0.096	1.258LysPhe: 1.258 ± 0.042	4.111LysGly: 4.111 ± 0.07	1.392LysHis: 1.392 ± 0.04	3.309LysIle: 3.309 ± 0.071	3.999LysLys: 3.999 ± 0.089	5.156LysLeu: 5.156 ± 0.099	1.559LysMet: 1.559 ± 0.045	1.854LysAsn: 1.854 ± 0.049	2.377LysPro: 2.377 ± 0.06	3.337LysGln: 3.337 ± 0.077	3.683LysArg: 3.683 ± 0.076	2.202LysSer: 2.202 ± 0.056	2.962LysThr: 2.962 ± 0.065	4.411LysVal: 4.411 ± 0.087	0.78LysTrp: 0.78 ± 0.032	1.708LysTyr: 1.708 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	9.521LeuAla: 9.521 ± 0.112	0.8LeuCys: 0.8 ± 0.03	5.365LeuAsp: 5.365 ± 0.085	7.412LeuGlu: 7.412 ± 0.116	4.706LeuPhe: 4.706 ± 0.1	7.3LeuGly: 7.3 ± 0.11	2.287LeuHis: 2.287 ± 0.055	6.934LeuIle: 6.934 ± 0.113	6.424LeuLys: 6.424 ± 0.092	10.702LeuLeu: 10.702 ± 0.172	2.473LeuMet: 2.473 ± 0.059	3.724LeuAsn: 3.724 ± 0.064	4.471LeuPro: 4.471 ± 0.068	3.787LeuGln: 3.787 ± 0.068	4.878LeuArg: 4.878 ± 0.079	6.32LeuSer: 6.32 ± 0.103	5.732LeuThr: 5.732 ± 0.084	7.182LeuVal: 7.182 ± 0.102	0.976LeuTrp: 0.976 ± 0.038	3.235LeuTyr: 3.235 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	2.534MetAla: 2.534 ± 0.053	0.154MetCys: 0.154 ± 0.013	1.354MetAsp: 1.354 ± 0.041	1.778MetGlu: 1.778 ± 0.045	0.969MetPhe: 0.969 ± 0.039	1.94MetGly: 1.94 ± 0.049	0.486MetHis: 0.486 ± 0.023	1.877MetIle: 1.877 ± 0.053	1.671MetLys: 1.671 ± 0.038	2.671MetLeu: 2.671 ± 0.06	0.857MetMet: 0.857 ± 0.034	1.039MetAsn: 1.039 ± 0.037	0.952MetPro: 0.952 ± 0.036	0.827MetGln: 0.827 ± 0.032	1.344MetArg: 1.344 ± 0.042	1.54MetSer: 1.54 ± 0.048	1.548MetThr: 1.548 ± 0.046	1.9MetVal: 1.9 ± 0.043	0.247MetTrp: 0.247 ± 0.017	0.688MetTyr: 0.688 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.082AsnAla: 2.082 ± 0.05	0.248AsnCys: 0.248 ± 0.017	1.399AsnAsp: 1.399 ± 0.049	2.246AsnGlu: 2.246 ± 0.057	1.103AsnPhe: 1.103 ± 0.037	2.502AsnGly: 2.502 ± 0.064	0.903AsnHis: 0.903 ± 0.035	2.321AsnIle: 2.321 ± 0.054	1.904AsnLys: 1.904 ± 0.051	3.132AsnLeu: 3.132 ± 0.065	0.975AsnMet: 0.975 ± 0.037	1.168AsnAsn: 1.168 ± 0.041	1.982AsnPro: 1.982 ± 0.049	1.619AsnGln: 1.619 ± 0.046	2.139AsnArg: 2.139 ± 0.048	1.29AsnSer: 1.29 ± 0.041	1.523AsnThr: 1.523 ± 0.044	2.489AsnVal: 2.489 ± 0.047	0.452AsnTrp: 0.452 ± 0.025	1.035AsnTyr: 1.035 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	3.207ProAla: 3.207 ± 0.058	0.335ProCys: 0.335 ± 0.02	2.622ProAsp: 2.622 ± 0.059	3.64ProGlu: 3.64 ± 0.074	2.133ProPhe: 2.133 ± 0.052	3.024ProGly: 3.024 ± 0.075	1.265ProHis: 1.265 ± 0.041	2.193ProIle: 2.193 ± 0.052	1.983ProLys: 1.983 ± 0.058	4.477ProLeu: 4.477 ± 0.069	0.898ProMet: 0.898 ± 0.034	1.362ProAsn: 1.362 ± 0.048	1.628ProPro: 1.628 ± 0.042	1.714ProGln: 1.714 ± 0.048	1.767ProArg: 1.767 ± 0.047	2.246ProSer: 2.246 ± 0.056	1.837ProThr: 1.837 ± 0.044	3.989ProVal: 3.989 ± 0.065	0.505ProTrp: 0.505 ± 0.026	1.619ProTyr: 1.619 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	4.371GlnAla: 4.371 ± 0.08	0.29GlnCys: 0.29 ± 0.018	1.99GlnAsp: 1.99 ± 0.053	3.582GlnGlu: 3.582 ± 0.081	1.676GlnPhe: 1.676 ± 0.044	3.066GlnGly: 3.066 ± 0.056	1.248GlnHis: 1.248 ± 0.041	2.589GlnIle: 2.589 ± 0.054	2.663GlnLys: 2.663 ± 0.065	5.079GlnLeu: 5.079 ± 0.103	1.223GlnMet: 1.223 ± 0.039	1.263GlnAsn: 1.263 ± 0.04	1.867GlnPro: 1.867 ± 0.052	2.532GlnGln: 2.532 ± 0.074	2.56GlnArg: 2.56 ± 0.065	1.937GlnSer: 1.937 ± 0.052	2.37GlnThr: 2.37 ± 0.053	3.53GlnVal: 3.53 ± 0.069	0.575GlnTrp: 0.575 ± 0.03	1.373GlnTyr: 1.373 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	3.499ArgAla: 3.499 ± 0.063	0.448ArgCys: 0.448 ± 0.021	2.572ArgAsp: 2.572 ± 0.061	4.345ArgGlu: 4.345 ± 0.079	2.467ArgPhe: 2.467 ± 0.064	3.173ArgGly: 3.173 ± 0.063	1.471ArgHis: 1.471 ± 0.05	3.51ArgIle: 3.51 ± 0.064	3.288ArgLys: 3.288 ± 0.064	6.278ArgLeu: 6.278 ± 0.092	1.643ArgMet: 1.643 ± 0.046	1.711ArgAsn: 1.711 ± 0.05	2.141ArgPro: 2.141 ± 0.052	2.842ArgGln: 2.842 ± 0.065	3.074ArgArg: 3.074 ± 0.058	2.436ArgSer: 2.436 ± 0.06	2.455ArgThr: 2.455 ± 0.058	3.529ArgVal: 3.529 ± 0.074	0.728ArgTrp: 0.728 ± 0.026	2.189ArgTyr: 2.189 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.645SerAla: 3.645 ± 0.078	0.43SerCys: 0.43 ± 0.021	2.004SerAsp: 2.004 ± 0.05	2.966SerGlu: 2.966 ± 0.06	2.367SerPhe: 2.367 ± 0.057	3.99SerGly: 3.99 ± 0.069	1.24SerHis: 1.24 ± 0.032	3.272SerIle: 3.272 ± 0.061	2.518SerLys: 2.518 ± 0.061	5.244SerLeu: 5.244 ± 0.091	1.417SerMet: 1.417 ± 0.035	1.412SerAsn: 1.412 ± 0.044	2.339SerPro: 2.339 ± 0.059	2.073SerGln: 2.073 ± 0.062	2.694SerArg: 2.694 ± 0.064	2.702SerSer: 2.702 ± 0.071	2.504SerThr: 2.504 ± 0.063	3.782SerVal: 3.782 ± 0.067	0.632SerTrp: 0.632 ± 0.028	1.584SerTyr: 1.584 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	4.085ThrAla: 4.085 ± 0.076	0.4ThrCys: 0.4 ± 0.02	2.349ThrAsp: 2.349 ± 0.05	3.385ThrGlu: 3.385 ± 0.07	2.311ThrPhe: 2.311 ± 0.056	4.429ThrGly: 4.429 ± 0.071	1.134ThrHis: 1.134 ± 0.038	3.588ThrIle: 3.588 ± 0.073	2.462ThrLys: 2.462 ± 0.053	5.471ThrLeu: 5.471 ± 0.095	1.182ThrMet: 1.182 ± 0.037	1.644ThrAsn: 1.644 ± 0.042	2.521ThrPro: 2.521 ± 0.054	1.707ThrGln: 1.707 ± 0.044	2.299ThrArg: 2.299 ± 0.052	2.551ThrSer: 2.551 ± 0.06	2.435ThrThr: 2.435 ± 0.057	4.246ThrVal: 4.246 ± 0.079	0.533ThrTrp: 0.533 ± 0.028	1.853ThrTyr: 1.853 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	6.095ValAla: 6.095 ± 0.098	0.735ValCys: 0.735 ± 0.03	3.859ValAsp: 3.859 ± 0.062	5.392ValGlu: 5.392 ± 0.085	3.006ValPhe: 3.006 ± 0.065	4.876ValGly: 4.876 ± 0.077	1.739ValHis: 1.739 ± 0.044	5.817ValIle: 5.817 ± 0.086	4.24ValLys: 4.24 ± 0.078	8.014ValLeu: 8.014 ± 0.116	2.095ValMet: 2.095 ± 0.05	2.655ValAsn: 2.655 ± 0.06	3.146ValPro: 3.146 ± 0.054	3.152ValGln: 3.152 ± 0.066	3.751ValArg: 3.751 ± 0.072	4.123ValSer: 4.123 ± 0.073	4.26ValThr: 4.26 ± 0.078	5.89ValVal: 5.89 ± 0.097	0.742ValTrp: 0.742 ± 0.033	2.655ValTyr: 2.655 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.823TrpAla: 0.823 ± 0.034	0.091TrpCys: 0.091 ± 0.011	0.551TrpAsp: 0.551 ± 0.025	0.794TrpGlu: 0.794 ± 0.033	0.536TrpPhe: 0.536 ± 0.029	0.768TrpGly: 0.768 ± 0.036	0.294TrpHis: 0.294 ± 0.02	0.754TrpIle: 0.754 ± 0.031	0.658TrpLys: 0.658 ± 0.029	1.577TrpLeu: 1.577 ± 0.045	0.379TrpMet: 0.379 ± 0.02	0.403TrpAsn: 0.403 ± 0.021	0.448TrpPro: 0.448 ± 0.022	0.662TrpGln: 0.662 ± 0.031	0.608TrpArg: 0.608 ± 0.031	0.567TrpSer: 0.567 ± 0.026	0.522TrpThr: 0.522 ± 0.024	0.791TrpVal: 0.791 ± 0.031	0.204TrpTrp: 0.204 ± 0.016	0.378TrpTyr: 0.378 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.414TyrAla: 2.414 ± 0.059	0.312TyrCys: 0.312 ± 0.02	1.86TyrAsp: 1.86 ± 0.055	2.288TyrGlu: 2.288 ± 0.057	1.609TyrPhe: 1.609 ± 0.053	2.496TyrGly: 2.496 ± 0.061	1.029TyrHis: 1.029 ± 0.036	2.121TyrIle: 2.121 ± 0.056	1.545TyrLys: 1.545 ± 0.042	3.653TyrLeu: 3.653 ± 0.075	0.722TyrMet: 0.722 ± 0.029	1.025TyrAsn: 1.025 ± 0.034	1.652TyrPro: 1.652 ± 0.045	1.701TyrGln: 1.701 ± 0.053	2.136TyrArg: 2.136 ± 0.05	1.493TyrSer: 1.493 ± 0.04	1.817TyrThr: 1.817 ± 0.046	2.581TyrVal: 2.581 ± 0.054	0.407TyrTrp: 0.407 ± 0.022	1.351TyrTyr: 1.351 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.016XaaXaa: 0.016 ± 0.011

Statistics based on 2969 proteins (837036 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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