Thiobaca trueperi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Thiobaca

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4R3N263|A0A4R3N263_9GAMM Serine-type D-Ala-D-Ala carboxypeptidase OS=Thiobaca trueperi OX=127458 GN=EDC35_103300 PE=3 SV=1
MM1 pKa = 7.67LAMNSLKK8 pKa = 10.82SMRR11 pKa = 11.84SPGTWRR17 pKa = 11.84AAGAALWLLSLILTGAPVTASILSTSPPVRR47 pKa = 11.84EE48 pKa = 4.04AAFIDD53 pKa = 3.59TGLADD58 pKa = 3.68WQVLRR63 pKa = 11.84DD64 pKa = 3.76GVKK67 pKa = 9.88PGVEE71 pKa = 4.29VVLLDD76 pKa = 4.33AGADD80 pKa = 3.73GLAQMAAWAQGRR92 pKa = 11.84SGYY95 pKa = 10.34DD96 pKa = 3.24AIHH99 pKa = 6.57LLSHH103 pKa = 6.56GNSGVIKK110 pKa = 10.6LGALTLSSDD119 pKa = 3.61NLQQHH124 pKa = 6.52SDD126 pKa = 3.02TLTRR130 pKa = 11.84IGSALTEE137 pKa = 4.83DD138 pKa = 4.29GDD140 pKa = 4.1FLVYY144 pKa = 10.33GCEE147 pKa = 3.95VAEE150 pKa = 4.43GQTGVEE156 pKa = 4.84FVRR159 pKa = 11.84KK160 pKa = 9.05LAQATGADD168 pKa = 3.76VAASDD173 pKa = 4.72DD174 pKa = 3.75LTGASGLGGDD184 pKa = 4.63WILEE188 pKa = 4.38SVLSLSSEE196 pKa = 4.05VSTPSIFIMAYY207 pKa = 9.64NQVLDD212 pKa = 4.41TIDD215 pKa = 4.19LLSHH219 pKa = 6.22GTSASPITWDD229 pKa = 3.5GTSGSDD235 pKa = 2.91IFSISIINDD244 pKa = 3.13NQYY247 pKa = 10.49EE248 pKa = 4.48YY249 pKa = 11.47AWGVASNKK257 pKa = 10.01GVGIEE262 pKa = 5.04DD263 pKa = 4.66EE264 pKa = 4.27IHH266 pKa = 4.98THH268 pKa = 6.94NIITNFQAATDD279 pKa = 3.91RR280 pKa = 11.84FDD282 pKa = 3.53FTSVIASFDD291 pKa = 3.54PGTVTFEE298 pKa = 4.02AQPTFLSSDD307 pKa = 4.05NILKK311 pKa = 8.2RR312 pKa = 11.84TPDD315 pKa = 3.01ITNVVIVTPAKK326 pKa = 10.2EE327 pKa = 4.27SAGDD331 pKa = 3.56ASTTATTGTDD341 pKa = 3.11GTFINVVGNNGDD353 pKa = 3.19WYY355 pKa = 11.16NFFLPGVIYY364 pKa = 10.58TDD366 pKa = 3.2LTINNFFPSVNTAPTLTATGIGTAFIEE393 pKa = 4.69GEE395 pKa = 4.05GGGIDD400 pKa = 3.58VFAGVTADD408 pKa = 3.51TGDD411 pKa = 3.45GSEE414 pKa = 4.38TFTGATLTVTGVTDD428 pKa = 3.3TTEE431 pKa = 3.85YY432 pKa = 10.99LIIAGTQVALTDD444 pKa = 3.83ATSGTITGVGSYY456 pKa = 9.67AVSMAGGTATVTLSGMTASNDD477 pKa = 3.54QMGGLIDD484 pKa = 5.03GIQYY488 pKa = 10.03FNSDD492 pKa = 3.15ASVTAGNRR500 pKa = 11.84VVTLTGITDD509 pKa = 3.84SGASSNSAVLNIAATVVVVDD529 pKa = 4.02VSNATLVVNVGTDD542 pKa = 3.07SDD544 pKa = 4.8SIGSSLANDD553 pKa = 3.57LTDD556 pKa = 4.21NGGLTLRR563 pKa = 11.84EE564 pKa = 3.99AMHH567 pKa = 6.48WADD570 pKa = 3.69FTGGADD576 pKa = 4.46FIEE579 pKa = 4.65LRR581 pKa = 11.84TNVTLNAANFDD592 pKa = 3.94LPFIRR597 pKa = 11.84GYY599 pKa = 8.26TLIKK603 pKa = 10.88GNGFKK608 pKa = 9.89IDD610 pKa = 3.29GAAYY614 pKa = 9.86AGFSMGEE621 pKa = 3.82QSGSRR626 pKa = 11.84MVLEE630 pKa = 5.73DD631 pKa = 3.56IEE633 pKa = 4.48LTNFSDD639 pKa = 4.95DD640 pKa = 3.61NNFFAGAAFLSLNAPGTLSAAIRR663 pKa = 11.84DD664 pKa = 3.52VSFYY668 pKa = 11.39GNNSTGGIGGGVVSVSADD686 pKa = 3.41TANTLILVDD695 pKa = 3.61IQNSRR700 pKa = 11.84FLNNDD705 pKa = 2.41VSNNKK710 pKa = 9.39GVIAVNSMNAGTAVVNVTSSAIYY733 pKa = 10.42DD734 pKa = 3.7NSGPAAIDD742 pKa = 3.87AYY744 pKa = 9.86TLGAGNSIYY753 pKa = 10.44MNVSNSSVAGNVVGVNLDD771 pKa = 3.7PAGGTISVNILNSIVVDD788 pKa = 3.39NTTANIVGSTNSQSSLTTNSSVNFVNSGSGDD819 pKa = 3.5YY820 pKa = 10.5RR821 pKa = 11.84LAAAASNALNVGDD834 pKa = 3.87AHH836 pKa = 6.66YY837 pKa = 9.2LTGSLDD843 pKa = 3.33VRR845 pKa = 11.84GLQRR849 pKa = 11.84VRR851 pKa = 11.84NGAVDD856 pKa = 3.49IGAYY860 pKa = 9.63EE861 pKa = 3.94SWLNGAAPSVDD872 pKa = 3.63VNGSTGGVNYY882 pKa = 8.11TNPASSGYY890 pKa = 5.85TTGITVVDD898 pKa = 4.05SAATVTQSDD907 pKa = 4.08SEE909 pKa = 4.3TRR911 pKa = 11.84IWKK914 pKa = 9.03MSAALTGNVDD924 pKa = 3.56GTSGADD930 pKa = 3.18EE931 pKa = 4.28TLFLSDD937 pKa = 4.61ANLLAAQQSGIAVTGNNTATLTLIGGDD964 pKa = 3.64TLADD968 pKa = 4.2FQAVLRR974 pKa = 11.84NIQYY978 pKa = 10.94KK979 pKa = 10.38NVDD982 pKa = 3.56STPTTGARR990 pKa = 11.84TVTITVDD997 pKa = 3.62DD998 pKa = 4.95DD999 pKa = 3.65ATASATATITVTTAVAPTVALSINNATVAEE1029 pKa = 4.23AAGSSTITATLSATASTDD1047 pKa = 3.19TTITLTPSGTATGSGTDD1064 pKa = 3.63YY1065 pKa = 10.11TLSSTTITITAGNTTGTATLSAVQDD1090 pKa = 3.94ALDD1093 pKa = 4.35EE1094 pKa = 4.85DD1095 pKa = 5.01DD1096 pKa = 4.06EE1097 pKa = 4.81TVILDD1102 pKa = 3.31ITGVSGGGGATEE1114 pKa = 5.11SGSQQQTITITDD1126 pKa = 4.19DD1127 pKa = 3.99DD1128 pKa = 4.46PTPSLSIADD1137 pKa = 3.62VSQAEE1142 pKa = 4.66GNSDD1146 pKa = 3.31TYY1148 pKa = 9.4MTFTVTLSAASGKK1161 pKa = 8.11TVTVNYY1167 pKa = 7.5ATSDD1171 pKa = 3.31GTATVGDD1178 pKa = 4.66DD1179 pKa = 3.48YY1180 pKa = 11.8AGVSDD1185 pKa = 4.54IPLSFAAGEE1194 pKa = 4.1TSKK1197 pKa = 9.7TFDD1200 pKa = 3.4VLIGGDD1206 pKa = 3.62TTTEE1210 pKa = 3.69ADD1212 pKa = 3.37EE1213 pKa = 4.6TFTVTLSNPSNATISDD1229 pKa = 3.46ATATGTITNDD1239 pKa = 4.54DD1240 pKa = 4.25IPNAAPTDD1248 pKa = 3.56ISLSNATLSVYY1259 pKa = 10.48DD1260 pKa = 4.06GSNATVGTLTSTDD1273 pKa = 3.3ANGGDD1278 pKa = 3.27THH1280 pKa = 7.24TYY1282 pKa = 9.1TLVSGTGDD1290 pKa = 3.16THH1292 pKa = 6.1NASFNISGDD1301 pKa = 3.58SLRR1304 pKa = 11.84ANDD1307 pKa = 4.81ASALTAGSYY1316 pKa = 8.51SVRR1319 pKa = 11.84IRR1321 pKa = 11.84STDD1324 pKa = 2.83SGAGHH1329 pKa = 7.17LTYY1332 pKa = 11.08AKK1334 pKa = 10.44AFTITVTDD1342 pKa = 3.65TLIVTVAAIDD1352 pKa = 3.62ASAPAGSYY1360 pKa = 10.42AADD1363 pKa = 3.82LADD1366 pKa = 5.33GGGLDD1371 pKa = 4.74LRR1373 pKa = 11.84EE1374 pKa = 4.23ALALAGGGGKK1384 pKa = 8.56TIGFDD1389 pKa = 3.43AGLSGQTITLGGGYY1403 pKa = 9.89AVAAGTTFDD1412 pKa = 4.25ADD1414 pKa = 3.94TVGTLTITGQTLSLAGAFSVTNGTGDD1440 pKa = 3.48TLTINSHH1447 pKa = 6.06LADD1450 pKa = 4.13NGSDD1454 pKa = 3.17TSALTKK1460 pKa = 10.7NGAGTLVLGGTNNTSSTGLNTIMASAGRR1488 pKa = 11.84LQISAATNLGTDD1500 pKa = 3.71GVTLSGGVTFATAGNTLTATNTFAIDD1526 pKa = 3.0TGGAFFNQTGGGHH1539 pKa = 6.2LTLSNQVSGDD1549 pKa = 3.38GLLTKK1554 pKa = 10.17TGGGSMTLSGNNDD1567 pKa = 3.14ALAGGVTINNGTVNATSTVEE1587 pKa = 3.89SLGYY1591 pKa = 10.8GSVTLGGGMLVLTDD1605 pKa = 4.42AGTIANDD1612 pKa = 3.21ITVSGAATISNSDD1625 pKa = 3.35AVTLSGVISGSGILTKK1641 pKa = 10.38TGAGNLTLAGANTYY1655 pKa = 10.85SGGTTVSSGTLIGTSTSLQGAMTNNATVEE1684 pKa = 4.19FAQAADD1690 pKa = 3.78GTYY1693 pKa = 10.28SGIMSGTGGLTKK1705 pKa = 10.27TGAGTLTLSGANTYY1719 pKa = 9.35TGATTVSAGTLLIQGSLAGSVAATAGTRR1747 pKa = 11.84LGATGGVGTTGSVATGALTIAAGGTLAAEE1776 pKa = 4.62INGTTAGTGYY1786 pKa = 10.92DD1787 pKa = 3.5QMNVTGAVNVSGATLALTLGFTPAAGNSFILINNDD1822 pKa = 2.67STDD1825 pKa = 3.05AMTGAFSTVTVGGTPVTVTSNRR1847 pKa = 11.84FTTGGLIYY1855 pKa = 9.99EE1856 pKa = 4.77ISTSGGTDD1864 pKa = 3.41SNDD1867 pKa = 3.1LTLTVVDD1874 pKa = 4.29TTAPTVQSVAAPANATYY1891 pKa = 10.53KK1892 pKa = 10.5IGDD1895 pKa = 3.74TLDD1898 pKa = 4.54FIVTFSEE1905 pKa = 4.16AVTISGTPSIALTLDD1920 pKa = 2.99TGGTVQANLNGTVTNSTTGTFRR1942 pKa = 11.84HH1943 pKa = 5.37TVIAGNLDD1951 pKa = 3.72LNGIGVATAITPNSATIRR1969 pKa = 11.84DD1970 pKa = 3.77AASNAMTNFAFTPPTTTGVLVDD1992 pKa = 3.94GVRR1995 pKa = 11.84PDD1997 pKa = 3.3KK1998 pKa = 11.2AAVNPITASDD2008 pKa = 3.62TDD2010 pKa = 3.41NDD2012 pKa = 3.85GTYY2015 pKa = 10.58DD2016 pKa = 3.48ASEE2019 pKa = 4.19TLTLTFTEE2027 pKa = 4.78AVDD2030 pKa = 3.58ATHH2033 pKa = 6.81VIVGNLTVSNGHH2045 pKa = 5.85SLGTGAGLTPNTGHH2059 pKa = 7.09AASFTLTLGTGPTLAVGDD2077 pKa = 4.36TLTLTAANVVDD2088 pKa = 3.84QAGNQASGDD2097 pKa = 3.81VVFTVPTLNAAPVASAVTLTGTAAAGQTLTGTYY2130 pKa = 9.42TFTDD2134 pKa = 3.49ANSDD2138 pKa = 3.73AEE2140 pKa = 4.33NTGGGGSSYY2149 pKa = 11.06RR2150 pKa = 11.84LVRR2153 pKa = 11.84SADD2156 pKa = 3.25NDD2158 pKa = 3.6VATTGDD2164 pKa = 3.29NTDD2167 pKa = 3.6AVTATATGGAPGSDD2181 pKa = 3.0TYY2183 pKa = 11.82VPVAADD2189 pKa = 3.18IGQYY2193 pKa = 10.94LFYY2196 pKa = 10.31CVTPAASAGTSPGSEE2211 pKa = 4.15VCSSAVGPVCLSATATVTSSADD2233 pKa = 3.14SGAGSLRR2240 pKa = 11.84QALVEE2245 pKa = 4.23VCGGGAIGFDD2255 pKa = 3.47ATTFAAPQTITLASEE2270 pKa = 4.15LTVGKK2275 pKa = 9.7HH2276 pKa = 3.72VTVNGGTARR2285 pKa = 11.84NVTVSGNNAVRR2296 pKa = 11.84VFHH2299 pKa = 6.83VNDD2302 pKa = 4.01GANLTLNDD2310 pKa = 3.83LSVVNGRR2317 pKa = 11.84VTATGGGGIDD2327 pKa = 3.75NGTGSLTLNRR2337 pKa = 11.84VSLADD2342 pKa = 3.65NVATGVNGGALYY2354 pKa = 9.43QAPGGSATLNEE2365 pKa = 4.32CLVRR2369 pKa = 11.84GNSTTASGGALFNDD2383 pKa = 4.14GTLSLRR2389 pKa = 11.84NTTVTSNHH2397 pKa = 6.39SDD2399 pKa = 3.41TNGGGLFNNLNATATVTNGTFQGNTATTAGGALFNHH2435 pKa = 7.21GSNASSNLTLKK2446 pKa = 9.24NTLLAGNTATNGTNCSGTVGNDD2468 pKa = 3.02GHH2470 pKa = 7.06NLDD2473 pKa = 5.65DD2474 pKa = 4.22GTSCAFGSANGSLSSTNPSLGTFDD2498 pKa = 3.55TTTGTFPLLAASPAIDD2514 pKa = 3.72AGTNTGCPTTDD2525 pKa = 2.72QRR2527 pKa = 11.84GIARR2531 pKa = 11.84PQGTSCDD2538 pKa = 2.89IGAYY2542 pKa = 9.89EE2543 pKa = 4.46EE2544 pKa = 4.42ASPINGSCGTANQPAISAPTTNLCAAGRR2572 pKa = 11.84ASPVSGSGPWAWSCQGLAGGTTASCAAPLRR2602 pKa = 11.84TWTATASVNGTGGSISPASQSVSHH2626 pKa = 6.41NARR2629 pKa = 11.84ASFAVTPATGYY2640 pKa = 10.96GIASVSGCGGSLSGSLYY2657 pKa = 7.21TTGPITDD2664 pKa = 3.73NTCTVSASFSQNAVAGVCGSDD2685 pKa = 2.92HH2686 pKa = 6.71GKK2688 pKa = 10.32ALSSTPTNLCATGSASGVSGTGPWTWTCAGTGGGAPANCAATTFHH2733 pKa = 6.64TVTATAGADD2742 pKa = 3.61GSISPTSRR2750 pKa = 11.84TVNDD2754 pKa = 3.75GGVTSFTVTPDD2765 pKa = 2.71NGFAIGTVSSDD2776 pKa = 3.24TCGGSLNGNLYY2787 pKa = 7.67TTGTVNAACTVTASFTATNTSTTIISVNPTPSKK2820 pKa = 9.81IGQPVTVSYY2829 pKa = 10.89SVSTPGAASDD2839 pKa = 4.08SVTVTAQDD2847 pKa = 3.4GTTCSGTAGGGSCTLTFNSAGTKK2870 pKa = 8.35TLIASYY2876 pKa = 9.72TGTAGKK2882 pKa = 10.11QNSASSGTNHH2892 pKa = 6.82LVADD2896 pKa = 4.72APSLATPSLNSGVVGVPYY2914 pKa = 11.07AMQLVASGGHH2924 pKa = 4.89VTTATPYY2931 pKa = 10.26RR2932 pKa = 11.84FNATGLPAGFSLSTTGLLSGTGNTAATSSVVITVTDD2968 pKa = 3.92ALNQTSPPQTLPLSLVNQLTVATTSLPAGLANARR3002 pKa = 11.84YY3003 pKa = 7.7EE3004 pKa = 4.22QTLEE3008 pKa = 3.99AVGGQTPYY3016 pKa = 11.02AWSLFSGSLPAGLTLDD3032 pKa = 3.77AATGEE3037 pKa = 4.24LSGTPTDD3044 pKa = 4.79LGASAPFTIRR3054 pKa = 11.84VTDD3057 pKa = 3.69AANRR3061 pKa = 11.84TADD3064 pKa = 3.43QALTLTTTAPTVVEE3078 pKa = 4.36TKK3080 pKa = 10.59NGSEE3084 pKa = 4.07VAANLTPEE3092 pKa = 4.06QGGNTCMLDD3101 pKa = 3.61DD3102 pKa = 4.14QQTLILNLGDD3112 pKa = 3.64SGAPAAPPTGTTLPYY3127 pKa = 11.0GLFKK3131 pKa = 10.66LTVQGCTPGQTRR3143 pKa = 11.84LTVRR3147 pKa = 11.84LVYY3150 pKa = 9.8PAALPAGTRR3159 pKa = 11.84YY3160 pKa = 8.81WKK3162 pKa = 10.45YY3163 pKa = 11.17GKK3165 pKa = 7.96TADD3168 pKa = 3.5NSADD3172 pKa = 2.99HH3173 pKa = 6.87WYY3175 pKa = 10.5VLPTAVIEE3183 pKa = 4.4GNTVSFTITDD3193 pKa = 3.69GGLGDD3198 pKa = 5.81DD3199 pKa = 4.81DD3200 pKa = 5.72LSANGSITDD3209 pKa = 3.83PGGPGGPVLGIGGAPGNGQVGSAYY3233 pKa = 10.43SAALSATSGTGPYY3246 pKa = 9.37NWSLAAGGLPPGVTLNPVTGALSGTPTLAGIFTFSVQLVDD3286 pKa = 3.45TGNGNTTVTQPYY3298 pKa = 9.72SVTIAAAGGGGPSPVNGTCGSAHH3321 pKa = 6.47NGLFYY3326 pKa = 10.7DD3327 pKa = 4.66LPTGALCASGTASAVSGSGPWTWSCLGSNGGSAASCRR3364 pKa = 11.84ANRR3367 pKa = 11.84ATATTYY3373 pKa = 10.35TVTTSASAGGTINPPSQVVNQGATATFTLTPATGYY3408 pKa = 10.83ALSSVSGCGGTLHH3421 pKa = 6.21GTTYY3425 pKa = 7.22TTGVITGACIVSATFSPRR3443 pKa = 11.84QYY3445 pKa = 10.45TVTASAGPGGTIAPASRR3462 pKa = 11.84RR3463 pKa = 11.84VSHH3466 pKa = 6.62GLTASFTLTPADD3478 pKa = 4.64GYY3480 pKa = 11.51AIDD3483 pKa = 4.11QVSGCGGRR3491 pKa = 11.84LSGTTYY3497 pKa = 7.77TTAPVTAACTVTARR3511 pKa = 11.84FAVTSTWLGLVGDD3524 pKa = 4.19WDD3526 pKa = 4.02GDD3528 pKa = 3.99GTVTAGLYY3536 pKa = 10.65DD3537 pKa = 3.83PVSGRR3542 pKa = 11.84FALRR3546 pKa = 11.84NANRR3550 pKa = 11.84TGPADD3555 pKa = 3.4SVFRR3559 pKa = 11.84YY3560 pKa = 10.58GPMNAGWWPLAGDD3573 pKa = 3.55WDD3575 pKa = 4.34GDD3577 pKa = 3.84GVTTVGLYY3585 pKa = 10.66DD3586 pKa = 3.93PLSGTFYY3593 pKa = 10.77LRR3595 pKa = 11.84NAHH3598 pKa = 5.71TPGVADD3604 pKa = 3.2VTFRR3608 pKa = 11.84YY3609 pKa = 9.88GPKK3612 pKa = 9.48NAGWYY3617 pKa = 8.99PLAGDD3622 pKa = 4.1WNHH3625 pKa = 7.17DD3626 pKa = 4.04GIDD3629 pKa = 3.7TVGLYY3634 pKa = 10.41DD3635 pKa = 3.79PRR3637 pKa = 11.84TGIFFLRR3644 pKa = 11.84NTHH3647 pKa = 5.46TPGVADD3653 pKa = 3.05QHH3655 pKa = 5.86FRR3657 pKa = 11.84YY3658 pKa = 10.34GPLDD3662 pKa = 4.31PGWIPLVGDD3671 pKa = 3.49WNGDD3675 pKa = 3.12GTTTIGLYY3683 pKa = 10.5DD3684 pKa = 3.84GLAGLVYY3691 pKa = 10.56LRR3693 pKa = 11.84QTNTPGVANVIFRR3706 pKa = 11.84YY3707 pKa = 10.48GPVDD3711 pKa = 3.7NDD3713 pKa = 2.66WWPLGGDD3720 pKa = 3.39WNGDD3724 pKa = 3.45DD3725 pKa = 4.38TDD3727 pKa = 4.26TIGLFKK3733 pKa = 10.38PATGTFYY3740 pKa = 11.1LRR3742 pKa = 11.84DD3743 pKa = 3.74SNTAGMAEE3751 pKa = 4.11YY3752 pKa = 9.78PDD3754 pKa = 3.99EE3755 pKa = 4.46NGFRR3759 pKa = 11.84ITTPSAATPP3768 pKa = 3.67

Molecular weight:
374.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4R3MXN3|A0A4R3MXN3_9GAMM 30S ribosomal protein S20 OS=Thiobaca trueperi OX=127458 GN=rpsT PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.48RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.93GFRR19 pKa = 11.84ARR21 pKa = 11.84SATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 9.31VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84ARR41 pKa = 11.84LIPP44 pKa = 4.0

Molecular weight:
5.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3588

0

3588

1205061

29

3768

335.9

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.395 ± 0.053

1.013 ± 0.017

5.966 ± 0.028

5.882 ± 0.044

3.507 ± 0.024

7.89 ± 0.044

2.274 ± 0.019

5.187 ± 0.032

2.617 ± 0.031

11.495 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.019

2.599 ± 0.031

5.303 ± 0.027

3.953 ± 0.03

7.725 ± 0.054

5.248 ± 0.04

5.279 ± 0.057

6.799 ± 0.035

1.391 ± 0.019

2.288 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski