Tortoise microvirus 82

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6N1|A0A4P8W6N1_9VIRU Uncharacterized protein OS=Tortoise microvirus 82 OX=2583190 PE=4 SV=1
MM1 pKa = 6.99QRR3 pKa = 11.84VFNIDD8 pKa = 2.45KK9 pKa = 7.42WTLVEE14 pKa = 4.45EE15 pKa = 4.77GQSLGFLNDD24 pKa = 2.87KK25 pKa = 9.47PRR27 pKa = 11.84NVRR30 pKa = 11.84LEE32 pKa = 4.11VNSPSEE38 pKa = 3.98VEE40 pKa = 4.14LWYY43 pKa = 10.51ACQVSGEE50 pKa = 4.22VHH52 pKa = 6.75FLALVKK58 pKa = 10.74GRR60 pKa = 11.84DD61 pKa = 3.63VVSFGSTGPFEE72 pKa = 4.1ITVKK76 pKa = 10.83NGFVMIATNDD86 pKa = 3.56GDD88 pKa = 4.32DD89 pKa = 3.74VSMKK93 pKa = 9.04PTAEE97 pKa = 4.04KK98 pKa = 10.95AFVKK102 pKa = 10.57VMEE105 pKa = 4.23RR106 pKa = 11.84RR107 pKa = 11.84ARR109 pKa = 11.84DD110 pKa = 3.32PHH112 pKa = 7.91LEE114 pKa = 3.71RR115 pKa = 11.84MMQLMEE121 pKa = 3.9VNMRR125 pKa = 11.84RR126 pKa = 11.84NQEE129 pKa = 3.66QQAYY133 pKa = 8.28EE134 pKa = 4.15LEE136 pKa = 4.11RR137 pKa = 11.84LFARR141 pKa = 11.84RR142 pKa = 11.84VAALEE147 pKa = 3.98AAASQGVGAGNVGDD161 pKa = 4.99AGAEE165 pKa = 3.86PAPQGTAGTAAKK177 pKa = 10.31GDD179 pKa = 3.72NSNGNDD185 pKa = 3.47AGHH188 pKa = 6.76DD189 pKa = 3.72QSASAAGGAA198 pKa = 3.86

Molecular weight: 21.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6U1|A0A4P8W6U1_9VIRU Uncharacterized protein OS=Tortoise microvirus 82 OX=2583190 PE=4 SV=1
MM1 pKa = 7.25AVWVGVSKK9 pKa = 10.48MKK11 pKa = 10.93LIGEE15 pKa = 4.27VLTRR19 pKa = 11.84RR20 pKa = 11.84VGTAVAAYY28 pKa = 10.07LVGTLGIPADD38 pKa = 3.69LAMKK42 pKa = 9.92FVLAGGVLLGIGADD56 pKa = 3.62VAIAAALARR65 pKa = 11.84FRR67 pKa = 4.58

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1865

67

547

266.4

29.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.831 ± 1.022

0.858 ± 0.38

5.094 ± 0.457

5.201 ± 0.528

3.7 ± 0.255

8.847 ± 0.757

2.735 ± 0.629

4.236 ± 0.431

3.914 ± 0.608

6.81 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.43

4.236 ± 0.515

6.166 ± 0.737

4.933 ± 0.569

7.614 ± 0.976

5.094 ± 0.667

5.308 ± 0.79

7.399 ± 0.662

1.716 ± 0.318

2.627 ± 0.619
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski