Desulfofundulus thermobenzoicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfofundulus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7IPL8|A0A6N7IPL8_9FIRM Type III glutamate--ammonia ligase OS=Desulfofundulus thermobenzoicus OX=29376 GN=glnT PE=3 SV=1
MM1 pKa = 6.91TCGVEE6 pKa = 4.03ILDD9 pKa = 3.6IQLLRR14 pKa = 11.84EE15 pKa = 3.68KK16 pKa = 10.04LLPVFEE22 pKa = 5.0SYY24 pKa = 10.91GVSCCYY30 pKa = 10.63LFGSRR35 pKa = 11.84AGEE38 pKa = 4.05NYY40 pKa = 10.33YY41 pKa = 10.5QDD43 pKa = 3.78SDD45 pKa = 3.3MDD47 pKa = 4.11LAVVFDD53 pKa = 4.21SYY55 pKa = 11.87SPEE58 pKa = 3.72KK59 pKa = 10.73HH60 pKa = 6.52NLDD63 pKa = 4.55LEE65 pKa = 4.36IEE67 pKa = 4.24IQDD70 pKa = 3.82TVSEE74 pKa = 4.05ILAPLEE80 pKa = 3.85VDD82 pKa = 4.93LLFLQKK88 pKa = 10.98APIYY92 pKa = 10.76LKK94 pKa = 9.9FTVIKK99 pKa = 9.31NGKK102 pKa = 9.18IIYY105 pKa = 9.7CSNEE109 pKa = 3.61DD110 pKa = 3.35FRR112 pKa = 11.84TDD114 pKa = 3.83FEE116 pKa = 5.33DD117 pKa = 3.32MTVRR121 pKa = 11.84DD122 pKa = 4.19YY123 pKa = 12.01LDD125 pKa = 4.17FKK127 pKa = 10.85PVLDD131 pKa = 3.76MYY133 pKa = 8.3YY134 pKa = 10.33QEE136 pKa = 4.22MAEE139 pKa = 4.98EE140 pKa = 4.41LMADD144 pKa = 3.15NDD146 pKa = 3.81GRR148 pKa = 11.84VV149 pKa = 2.94

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7IT30|A0A6N7IT30_9FIRM Nitro_FeMo-Co domain-containing protein OS=Desulfofundulus thermobenzoicus OX=29376 GN=GFC01_13395 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.64QPKK8 pKa = 8.16ARR10 pKa = 11.84KK11 pKa = 8.84RR12 pKa = 11.84KK13 pKa = 8.98RR14 pKa = 11.84VHH16 pKa = 6.35GFLKK20 pKa = 10.61RR21 pKa = 11.84MSTKK25 pKa = 10.43AGRR28 pKa = 11.84NVIKK32 pKa = 10.26RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84MKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 9.06RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3512

0

3512

1013857

22

3893

288.7

31.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.562 ± 0.046

1.257 ± 0.022

4.668 ± 0.029

6.886 ± 0.048

3.594 ± 0.031

8.542 ± 0.041

1.959 ± 0.017

5.666 ± 0.04

4.457 ± 0.037

10.686 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.019

3.04 ± 0.026

4.985 ± 0.024

3.412 ± 0.026

7.154 ± 0.043

4.601 ± 0.024

4.818 ± 0.029

8.189 ± 0.04

1.09 ± 0.018

2.93 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski