Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87)

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Thermodesulfovibrionia; Thermodesulfovibrionales; Thermodesulfovibrionaceae; Thermodesulfovibrio; Thermodesulfovibrio yellowstonii

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5YK69|B5YK69_THEYD 30S ribosomal protein S6 OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) OX=289376 GN=rpsF PE=3 SV=1
MM1 pKa = 7.16PTPVVDD7 pKa = 3.68YY8 pKa = 9.75DD9 pKa = 3.91LCVGCGSCAEE19 pKa = 4.13VCPEE23 pKa = 3.91VFEE26 pKa = 4.63MRR28 pKa = 11.84DD29 pKa = 3.25DD30 pKa = 3.98KK31 pKa = 11.66AWVVGPDD38 pKa = 3.03KK39 pKa = 11.2CSTCDD44 pKa = 3.4CQQAADD50 pKa = 4.6LCPSQAIRR58 pKa = 11.84LEE60 pKa = 4.09

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5YHS0|B5YHS0_THEYD Probable membrane transporter protein OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) OX=289376 GN=THEYE_A1866 PE=3 SV=1
MM1 pKa = 7.78PGFDD5 pKa = 2.86RR6 pKa = 11.84TGPFGQGPMTGRR18 pKa = 11.84GMGYY22 pKa = 9.75CGGASRR28 pKa = 11.84YY29 pKa = 7.65MNSASGRR36 pKa = 11.84RR37 pKa = 11.84FGFGRR42 pKa = 11.84GFRR45 pKa = 11.84CLGGFRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84FRR55 pKa = 11.84WLWRR59 pKa = 11.84MPFFGGASSRR69 pKa = 11.84EE70 pKa = 3.75EE71 pKa = 4.06VEE73 pKa = 3.9WLKK76 pKa = 11.35EE77 pKa = 3.46EE78 pKa = 4.53AEE80 pKa = 3.95ILKK83 pKa = 10.5RR84 pKa = 11.84EE85 pKa = 4.13LEE87 pKa = 4.18AVQRR91 pKa = 11.84RR92 pKa = 11.84LSEE95 pKa = 3.95IEE97 pKa = 3.99KK98 pKa = 10.46EE99 pKa = 4.13KK100 pKa = 11.52AEE102 pKa = 4.06

Molecular weight: 11.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1982

0

1982

608875

37

1843

307.2

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.144 ± 0.056

1.109 ± 0.023

4.692 ± 0.034

7.9 ± 0.062

4.984 ± 0.049

6.356 ± 0.051

1.613 ± 0.022

9.946 ± 0.059

9.189 ± 0.055

9.679 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.025

4.308 ± 0.04

3.82 ± 0.035

2.718 ± 0.031

4.15 ± 0.038

5.761 ± 0.04

4.654 ± 0.037

6.221 ± 0.047

0.87 ± 0.019

3.656 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski