Streptomyces sp. H-KF8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A5PG58|A0A1A5PG58_9ACTN Uncharacterized protein OS=Streptomyces sp. H-KF8 OX=1727216 GN=A4U61_00345 PE=4 SV=1
MM1 pKa = 7.31GAAGCEE7 pKa = 3.94AGDD10 pKa = 3.93DD11 pKa = 3.66HH12 pKa = 7.18GVAYY16 pKa = 10.19VVVQAAEE23 pKa = 4.01AEE25 pKa = 4.29VRR27 pKa = 11.84EE28 pKa = 4.3GAEE31 pKa = 3.66AGGAQVGRR39 pKa = 11.84EE40 pKa = 3.97AVVAGCGDD48 pKa = 3.76LVDD51 pKa = 4.39ASGPGGGYY59 pKa = 9.17PDD61 pKa = 3.42QAAVFVGEE69 pKa = 4.27GEE71 pKa = 4.18EE72 pKa = 4.36VQAVMAVFAGVVPPVGSSGAALGGDD97 pKa = 3.48EE98 pKa = 4.41GAVDD102 pKa = 4.44EE103 pKa = 5.12DD104 pKa = 4.9CFPASLGDD112 pKa = 3.92LLQGAVQARR121 pKa = 11.84SWGVEE126 pKa = 3.49QRR128 pKa = 11.84DD129 pKa = 3.73QLFAPAADD137 pKa = 3.91GGLGHH142 pKa = 7.28IAAAGYY148 pKa = 9.57VGQALVVAQHH158 pKa = 6.1GQDD161 pKa = 3.29DD162 pKa = 4.08HH163 pKa = 8.24

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A5P1F1|A0A1A5P1F1_9ACTN Adenylosuccinate synthetase OS=Streptomyces sp. H-KF8 OX=1727216 GN=purA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6118

0

6118

2010856

29

6666

328.7

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.304 ± 0.049

0.76 ± 0.009

6.139 ± 0.026

5.954 ± 0.03

2.695 ± 0.018

9.732 ± 0.034

2.354 ± 0.015

2.96 ± 0.024

2.11 ± 0.027

10.228 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.706 ± 0.012

1.704 ± 0.018

6.236 ± 0.031

2.597 ± 0.022

8.356 ± 0.038

4.973 ± 0.027

6.183 ± 0.029

8.514 ± 0.035

1.467 ± 0.013

2.029 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski