Piromyces finnis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Neocallimastigomycetes; Neocallimastigales; Neocallimastigaceae; Piromyces

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1UTX2|A0A1Y1UTX2_9FUNG Uncharacterized protein OS=Piromyces finnis OX=1754191 GN=BCR36DRAFT_181627 PE=4 SV=1
MM1 pKa = 6.81EE2 pKa = 5.0TLYY5 pKa = 10.67QYY7 pKa = 11.43NIFNSSVPTEE17 pKa = 4.38PIMDD21 pKa = 3.67NTILKK26 pKa = 9.54SKK28 pKa = 10.57KK29 pKa = 9.72IIKK32 pKa = 9.51QKK34 pKa = 10.23NFKK37 pKa = 9.75KK38 pKa = 10.6VHH40 pKa = 4.9FAEE43 pKa = 5.11NITLEE48 pKa = 4.2YY49 pKa = 9.67TYY51 pKa = 11.35SSDD54 pKa = 3.58IYY56 pKa = 11.22DD57 pKa = 3.53RR58 pKa = 11.84TPFEE62 pKa = 5.05DD63 pKa = 4.66DD64 pKa = 4.07DD65 pKa = 5.5YY66 pKa = 12.13EE67 pKa = 4.41MEE69 pKa = 4.98CSNTIQKK76 pKa = 10.01PVVFNQILDD85 pKa = 4.74DD86 pKa = 3.78IDD88 pKa = 4.99FEE90 pKa = 5.52IIYY93 pKa = 10.57NNNNVHH99 pKa = 5.36LTIYY103 pKa = 8.26NTNSYY108 pKa = 10.86EE109 pKa = 5.61DD110 pKa = 4.8DD111 pKa = 4.41DD112 pKa = 4.41QDD114 pKa = 4.81SVIDD118 pKa = 4.4FDD120 pKa = 5.28DD121 pKa = 5.03FEE123 pKa = 5.85DD124 pKa = 5.82FEE126 pKa = 6.22DD127 pKa = 4.59FDD129 pKa = 6.37DD130 pKa = 4.23YY131 pKa = 12.11AFF133 pKa = 4.49

Molecular weight: 15.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1VIL2|A0A1Y1VIL2_9FUNG M20_dimer domain-containing protein OS=Piromyces finnis OX=1754191 GN=BCR36DRAFT_580661 PE=3 SV=1
MM1 pKa = 7.89PSNKK5 pKa = 8.25TFKK8 pKa = 10.22IKK10 pKa = 10.55RR11 pKa = 11.84ILGKK15 pKa = 9.62KK16 pKa = 8.73QKK18 pKa = 9.07QNRR21 pKa = 11.84PLPQWFRR28 pKa = 11.84FKK30 pKa = 10.23TGNTIRR36 pKa = 11.84YY37 pKa = 5.99NAKK40 pKa = 8.74RR41 pKa = 11.84RR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 11.05LNLL51 pKa = 3.44

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10768

19

10787

5648188

49

11369

523.6

60.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.677 ± 0.022

1.464 ± 0.015

5.773 ± 0.019

7.425 ± 0.04

4.121 ± 0.02

3.95 ± 0.027

1.694 ± 0.012

8.767 ± 0.032

9.082 ± 0.037

8.247 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.993 ± 0.011

9.965 ± 0.049

3.464 ± 0.021

3.495 ± 0.018

3.175 ± 0.019

8.625 ± 0.04

5.429 ± 0.028

4.483 ± 0.019

0.771 ± 0.008

4.4 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski