Amino acid dipepetide frequency for Gordonia phage Schmidt

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.848AlaAla: 16.848 ± 1.471
0.802AlaCys: 0.802 ± 0.262
8.387AlaAsp: 8.387 ± 0.893
8.825AlaGlu: 8.825 ± 0.899
2.407AlaPhe: 2.407 ± 0.37
10.43AlaGly: 10.43 ± 1.286
1.896AlaHis: 1.896 ± 0.308
4.23AlaIle: 4.23 ± 0.57
4.449AlaLys: 4.449 ± 0.669
8.242AlaLeu: 8.242 ± 0.871
3.209AlaMet: 3.209 ± 0.479
2.99AlaAsn: 2.99 ± 0.451
3.866AlaPro: 3.866 ± 0.501
5.762AlaGln: 5.762 ± 0.477
7.95AlaArg: 7.95 ± 0.844
4.887AlaSer: 4.887 ± 0.588
6.856AlaThr: 6.856 ± 0.919
9.117AlaVal: 9.117 ± 1.037
2.261AlaTrp: 2.261 ± 0.321
2.699AlaTyr: 2.699 ± 0.576
0.0AlaXaa: 0.0 ± 0.0
Cys
0.729CysAla: 0.729 ± 0.238
0.0CysCys: 0.0 ± 0.0
0.583CysAsp: 0.583 ± 0.301
0.948CysGlu: 0.948 ± 0.363
0.146CysPhe: 0.146 ± 0.106
1.021CysGly: 1.021 ± 0.339
0.292CysHis: 0.292 ± 0.144
0.583CysIle: 0.583 ± 0.208
0.219CysLys: 0.219 ± 0.127
0.219CysLeu: 0.219 ± 0.113
0.073CysMet: 0.073 ± 0.067
0.729CysAsn: 0.729 ± 0.234
0.729CysPro: 0.729 ± 0.235
0.292CysGln: 0.292 ± 0.143
0.365CysArg: 0.365 ± 0.187
0.365CysSer: 0.365 ± 0.141
0.292CysThr: 0.292 ± 0.136
0.511CysVal: 0.511 ± 0.196
0.365CysTrp: 0.365 ± 0.152
0.219CysTyr: 0.219 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
7.95AspAla: 7.95 ± 0.687
1.24AspCys: 1.24 ± 0.292
4.96AspAsp: 4.96 ± 0.63
4.595AspGlu: 4.595 ± 0.781
1.459AspPhe: 1.459 ± 0.274
7.804AspGly: 7.804 ± 0.66
1.605AspHis: 1.605 ± 0.29
0.948AspIle: 0.948 ± 0.301
2.261AspLys: 2.261 ± 0.491
5.251AspLeu: 5.251 ± 0.634
1.167AspMet: 1.167 ± 0.253
1.459AspAsn: 1.459 ± 0.361
3.501AspPro: 3.501 ± 0.488
2.48AspGln: 2.48 ± 0.58
5.251AspArg: 5.251 ± 0.684
2.917AspSer: 2.917 ± 0.366
2.99AspThr: 2.99 ± 0.493
4.595AspVal: 4.595 ± 0.609
1.75AspTrp: 1.75 ± 0.34
2.626AspTyr: 2.626 ± 0.52
0.0AspXaa: 0.0 ± 0.0
Glu
5.616GluAla: 5.616 ± 0.651
0.365GluCys: 0.365 ± 0.151
3.938GluAsp: 3.938 ± 0.401
2.042GluGlu: 2.042 ± 0.33
3.355GluPhe: 3.355 ± 0.466
5.105GluGly: 5.105 ± 0.619
1.459GluHis: 1.459 ± 0.313
1.459GluIle: 1.459 ± 0.297
1.605GluLys: 1.605 ± 0.38
7.075GluLeu: 7.075 ± 0.638
1.532GluMet: 1.532 ± 0.326
1.24GluAsn: 1.24 ± 0.316
3.793GluPro: 3.793 ± 0.664
3.793GluGln: 3.793 ± 0.587
5.616GluArg: 5.616 ± 0.691
3.72GluSer: 3.72 ± 0.507
3.793GluThr: 3.793 ± 0.599
5.616GluVal: 5.616 ± 0.727
1.677GluTrp: 1.677 ± 0.391
2.48GluTyr: 2.48 ± 0.542
0.0GluXaa: 0.0 ± 0.0
Phe
2.917PheAla: 2.917 ± 0.416
0.292PheCys: 0.292 ± 0.128
1.386PheAsp: 1.386 ± 0.329
2.115PheGlu: 2.115 ± 0.48
0.802PhePhe: 0.802 ± 0.216
2.188PheGly: 2.188 ± 0.552
0.292PheHis: 0.292 ± 0.137
0.729PheIle: 0.729 ± 0.23
1.313PheLys: 1.313 ± 0.298
1.386PheLeu: 1.386 ± 0.364
0.656PheMet: 0.656 ± 0.232
0.438PheAsn: 0.438 ± 0.183
0.438PhePro: 0.438 ± 0.232
1.386PheGln: 1.386 ± 0.254
3.574PheArg: 3.574 ± 0.422
1.386PheSer: 1.386 ± 0.312
2.917PheThr: 2.917 ± 0.432
1.896PheVal: 1.896 ± 0.394
0.292PheTrp: 0.292 ± 0.178
0.583PheTyr: 0.583 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
8.679GlyAla: 8.679 ± 1.022
0.729GlyCys: 0.729 ± 0.22
7.877GlyAsp: 7.877 ± 0.93
6.418GlyGlu: 6.418 ± 0.746
2.48GlyPhe: 2.48 ± 0.492
11.597GlyGly: 11.597 ± 1.334
2.334GlyHis: 2.334 ± 0.501
4.157GlyIle: 4.157 ± 0.588
3.501GlyLys: 3.501 ± 0.512
6.345GlyLeu: 6.345 ± 0.624
2.48GlyMet: 2.48 ± 0.465
2.626GlyAsn: 2.626 ± 0.547
3.574GlyPro: 3.574 ± 0.745
4.23GlyGln: 4.23 ± 0.44
6.929GlyArg: 6.929 ± 0.695
5.543GlySer: 5.543 ± 0.813
5.762GlyThr: 5.762 ± 0.764
6.783GlyVal: 6.783 ± 1.101
1.459GlyTrp: 1.459 ± 0.334
2.334GlyTyr: 2.334 ± 0.456
0.0GlyXaa: 0.0 ± 0.0
His
2.626HisAla: 2.626 ± 0.416
0.583HisCys: 0.583 ± 0.278
1.021HisAsp: 1.021 ± 0.326
1.677HisGlu: 1.677 ± 0.435
0.292HisPhe: 0.292 ± 0.128
2.115HisGly: 2.115 ± 0.408
0.656HisHis: 0.656 ± 0.223
0.583HisIle: 0.583 ± 0.213
0.729HisLys: 0.729 ± 0.22
1.021HisLeu: 1.021 ± 0.282
1.094HisMet: 1.094 ± 0.263
0.729HisAsn: 0.729 ± 0.243
1.386HisPro: 1.386 ± 0.318
0.802HisGln: 0.802 ± 0.25
1.167HisArg: 1.167 ± 0.278
1.021HisSer: 1.021 ± 0.283
0.729HisThr: 0.729 ± 0.221
2.188HisVal: 2.188 ± 0.377
0.656HisTrp: 0.656 ± 0.182
0.438HisTyr: 0.438 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
5.543IleAla: 5.543 ± 0.612
0.438IleCys: 0.438 ± 0.162
2.407IleAsp: 2.407 ± 0.397
3.793IleGlu: 3.793 ± 0.533
0.292IlePhe: 0.292 ± 0.132
3.574IleGly: 3.574 ± 0.589
0.875IleHis: 0.875 ± 0.267
0.875IleIle: 0.875 ± 0.319
1.094IleLys: 1.094 ± 0.329
1.823IleLeu: 1.823 ± 0.381
0.438IleMet: 0.438 ± 0.166
0.656IleAsn: 0.656 ± 0.198
1.677IlePro: 1.677 ± 0.34
1.459IleGln: 1.459 ± 0.365
1.969IleArg: 1.969 ± 0.476
1.021IleSer: 1.021 ± 0.243
2.115IleThr: 2.115 ± 0.348
3.793IleVal: 3.793 ± 0.495
0.292IleTrp: 0.292 ± 0.142
0.438IleTyr: 0.438 ± 0.18
0.0IleXaa: 0.0 ± 0.0
Lys
5.178LysAla: 5.178 ± 0.694
0.146LysCys: 0.146 ± 0.103
2.407LysAsp: 2.407 ± 0.383
0.583LysGlu: 0.583 ± 0.195
0.656LysPhe: 0.656 ± 0.237
2.844LysGly: 2.844 ± 0.411
0.948LysHis: 0.948 ± 0.265
0.729LysIle: 0.729 ± 0.228
1.021LysLys: 1.021 ± 0.259
3.136LysLeu: 3.136 ± 0.496
0.875LysMet: 0.875 ± 0.206
0.948LysAsn: 0.948 ± 0.376
1.969LysPro: 1.969 ± 0.393
0.511LysGln: 0.511 ± 0.194
3.063LysArg: 3.063 ± 0.43
2.48LysSer: 2.48 ± 0.527
2.261LysThr: 2.261 ± 0.452
3.428LysVal: 3.428 ± 0.615
0.656LysTrp: 0.656 ± 0.243
1.167LysTyr: 1.167 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
10.794LeuAla: 10.794 ± 0.974
0.583LeuCys: 0.583 ± 0.197
5.47LeuAsp: 5.47 ± 0.632
5.324LeuGlu: 5.324 ± 0.726
2.115LeuPhe: 2.115 ± 0.401
7.293LeuGly: 7.293 ± 0.674
1.75LeuHis: 1.75 ± 0.37
2.407LeuIle: 2.407 ± 0.424
2.334LeuLys: 2.334 ± 0.478
5.981LeuLeu: 5.981 ± 0.657
1.677LeuMet: 1.677 ± 0.361
1.75LeuAsn: 1.75 ± 0.439
5.032LeuPro: 5.032 ± 0.719
2.188LeuGln: 2.188 ± 0.385
5.47LeuArg: 5.47 ± 0.663
3.647LeuSer: 3.647 ± 0.447
4.887LeuThr: 4.887 ± 0.711
6.272LeuVal: 6.272 ± 0.562
1.386LeuTrp: 1.386 ± 0.3
2.48LeuTyr: 2.48 ± 0.418
0.0LeuXaa: 0.0 ± 0.0
Met
3.428MetAla: 3.428 ± 0.434
0.073MetCys: 0.073 ± 0.088
1.677MetAsp: 1.677 ± 0.344
0.875MetGlu: 0.875 ± 0.243
0.219MetPhe: 0.219 ± 0.12
1.313MetGly: 1.313 ± 0.385
0.365MetHis: 0.365 ± 0.126
0.948MetIle: 0.948 ± 0.237
1.094MetLys: 1.094 ± 0.288
1.677MetLeu: 1.677 ± 0.363
0.365MetMet: 0.365 ± 0.142
0.875MetAsn: 0.875 ± 0.23
1.313MetPro: 1.313 ± 0.341
0.802MetGln: 0.802 ± 0.254
1.896MetArg: 1.896 ± 0.329
1.896MetSer: 1.896 ± 0.315
2.042MetThr: 2.042 ± 0.462
1.532MetVal: 1.532 ± 0.365
0.365MetTrp: 0.365 ± 0.155
0.511MetTyr: 0.511 ± 0.213
0.0MetXaa: 0.0 ± 0.0
Asn
3.355AsnAla: 3.355 ± 0.482
0.365AsnCys: 0.365 ± 0.264
1.459AsnAsp: 1.459 ± 0.32
1.313AsnGlu: 1.313 ± 0.317
0.948AsnPhe: 0.948 ± 0.245
3.574AsnGly: 3.574 ± 0.488
0.365AsnHis: 0.365 ± 0.165
0.729AsnIle: 0.729 ± 0.25
1.021AsnLys: 1.021 ± 0.224
2.042AsnLeu: 2.042 ± 0.385
0.656AsnMet: 0.656 ± 0.208
0.292AsnAsn: 0.292 ± 0.155
2.188AsnPro: 2.188 ± 0.321
0.802AsnGln: 0.802 ± 0.258
1.677AsnArg: 1.677 ± 0.345
1.094AsnSer: 1.094 ± 0.284
1.167AsnThr: 1.167 ± 0.312
1.969AsnVal: 1.969 ± 0.346
0.438AsnTrp: 0.438 ± 0.199
0.656AsnTyr: 0.656 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
4.23ProAla: 4.23 ± 0.609
0.511ProCys: 0.511 ± 0.167
4.376ProAsp: 4.376 ± 0.579
4.522ProGlu: 4.522 ± 0.594
1.24ProPhe: 1.24 ± 0.313
4.522ProGly: 4.522 ± 0.605
1.24ProHis: 1.24 ± 0.313
1.823ProIle: 1.823 ± 0.425
1.605ProLys: 1.605 ± 0.399
2.917ProLeu: 2.917 ± 0.46
0.583ProMet: 0.583 ± 0.189
1.605ProAsn: 1.605 ± 0.309
1.459ProPro: 1.459 ± 0.345
2.188ProGln: 2.188 ± 0.408
2.188ProArg: 2.188 ± 0.415
2.115ProSer: 2.115 ± 0.379
3.209ProThr: 3.209 ± 0.656
3.72ProVal: 3.72 ± 0.455
1.459ProTrp: 1.459 ± 0.386
1.021ProTyr: 1.021 ± 0.226
0.0ProXaa: 0.0 ± 0.0
Gln
5.47GlnAla: 5.47 ± 0.607
0.219GlnCys: 0.219 ± 0.107
2.042GlnAsp: 2.042 ± 0.426
1.24GlnGlu: 1.24 ± 0.273
1.677GlnPhe: 1.677 ± 0.317
2.917GlnGly: 2.917 ± 0.497
0.438GlnHis: 0.438 ± 0.149
1.896GlnIle: 1.896 ± 0.368
1.021GlnLys: 1.021 ± 0.276
5.324GlnLeu: 5.324 ± 0.623
1.094GlnMet: 1.094 ± 0.33
1.167GlnAsn: 1.167 ± 0.219
2.042GlnPro: 2.042 ± 0.402
1.605GlnGln: 1.605 ± 0.343
3.209GlnArg: 3.209 ± 0.519
1.677GlnSer: 1.677 ± 0.305
2.48GlnThr: 2.48 ± 0.428
2.844GlnVal: 2.844 ± 0.397
1.167GlnTrp: 1.167 ± 0.3
1.386GlnTyr: 1.386 ± 0.362
0.0GlnXaa: 0.0 ± 0.0
Arg
7.877ArgAla: 7.877 ± 0.974
0.656ArgCys: 0.656 ± 0.216
5.616ArgAsp: 5.616 ± 0.627
4.887ArgGlu: 4.887 ± 0.618
2.261ArgPhe: 2.261 ± 0.422
6.345ArgGly: 6.345 ± 0.827
1.677ArgHis: 1.677 ± 0.323
3.136ArgIle: 3.136 ± 0.449
3.209ArgLys: 3.209 ± 0.521
5.397ArgLeu: 5.397 ± 0.601
1.532ArgMet: 1.532 ± 0.311
2.188ArgAsn: 2.188 ± 0.349
2.626ArgPro: 2.626 ± 0.473
3.574ArgGln: 3.574 ± 0.563
7.148ArgArg: 7.148 ± 0.811
3.647ArgSer: 3.647 ± 0.562
5.032ArgThr: 5.032 ± 0.556
6.564ArgVal: 6.564 ± 0.733
1.969ArgTrp: 1.969 ± 0.37
2.115ArgTyr: 2.115 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
5.324SerAla: 5.324 ± 0.975
0.219SerCys: 0.219 ± 0.133
2.771SerAsp: 2.771 ± 0.4
2.99SerGlu: 2.99 ± 0.497
1.021SerPhe: 1.021 ± 0.269
6.637SerGly: 6.637 ± 0.73
0.875SerHis: 0.875 ± 0.228
1.896SerIle: 1.896 ± 0.431
1.094SerLys: 1.094 ± 0.274
4.668SerLeu: 4.668 ± 0.56
1.459SerMet: 1.459 ± 0.395
1.094SerAsn: 1.094 ± 0.197
2.188SerPro: 2.188 ± 0.478
1.605SerGln: 1.605 ± 0.31
3.793SerArg: 3.793 ± 0.542
2.261SerSer: 2.261 ± 0.522
3.355SerThr: 3.355 ± 0.593
5.032SerVal: 5.032 ± 0.843
1.021SerTrp: 1.021 ± 0.27
0.875SerTyr: 0.875 ± 0.236
0.0SerXaa: 0.0 ± 0.0
Thr
6.564ThrAla: 6.564 ± 0.738
0.511ThrCys: 0.511 ± 0.19
3.72ThrAsp: 3.72 ± 0.495
3.428ThrGlu: 3.428 ± 0.492
1.75ThrPhe: 1.75 ± 0.317
6.199ThrGly: 6.199 ± 1.157
1.313ThrHis: 1.313 ± 0.394
3.793ThrIle: 3.793 ± 0.541
2.626ThrLys: 2.626 ± 0.428
4.084ThrLeu: 4.084 ± 0.55
1.021ThrMet: 1.021 ± 0.271
1.167ThrAsn: 1.167 ± 0.341
3.136ThrPro: 3.136 ± 0.512
1.677ThrGln: 1.677 ± 0.314
4.741ThrArg: 4.741 ± 0.631
2.553ThrSer: 2.553 ± 0.404
2.699ThrThr: 2.699 ± 0.415
4.887ThrVal: 4.887 ± 0.609
1.386ThrTrp: 1.386 ± 0.31
1.896ThrTyr: 1.896 ± 0.544
0.0ThrXaa: 0.0 ± 0.0
Val
8.898ValAla: 8.898 ± 0.847
0.365ValCys: 0.365 ± 0.172
4.303ValAsp: 4.303 ± 0.614
6.418ValGlu: 6.418 ± 0.691
2.553ValPhe: 2.553 ± 0.363
5.835ValGly: 5.835 ± 0.827
2.042ValHis: 2.042 ± 0.375
2.407ValIle: 2.407 ± 0.415
2.917ValLys: 2.917 ± 0.482
8.169ValLeu: 8.169 ± 0.764
2.334ValMet: 2.334 ± 0.449
2.334ValAsn: 2.334 ± 0.374
3.428ValPro: 3.428 ± 0.472
4.011ValGln: 4.011 ± 0.497
6.054ValArg: 6.054 ± 0.805
4.96ValSer: 4.96 ± 0.826
4.449ValThr: 4.449 ± 0.547
5.397ValVal: 5.397 ± 0.697
1.605ValTrp: 1.605 ± 0.382
1.021ValTyr: 1.021 ± 0.293
0.0ValXaa: 0.0 ± 0.0
Trp
1.677TrpAla: 1.677 ± 0.456
0.292TrpCys: 0.292 ± 0.136
0.948TrpAsp: 0.948 ± 0.253
1.459TrpGlu: 1.459 ± 0.272
0.511TrpPhe: 0.511 ± 0.208
1.386TrpGly: 1.386 ± 0.277
0.656TrpHis: 0.656 ± 0.222
0.802TrpIle: 0.802 ± 0.229
0.875TrpLys: 0.875 ± 0.236
2.553TrpLeu: 2.553 ± 0.439
0.365TrpMet: 0.365 ± 0.137
0.802TrpAsn: 0.802 ± 0.292
0.875TrpPro: 0.875 ± 0.362
1.313TrpGln: 1.313 ± 0.315
2.115TrpArg: 2.115 ± 0.38
1.532TrpSer: 1.532 ± 0.404
1.313TrpThr: 1.313 ± 0.324
1.021TrpVal: 1.021 ± 0.262
0.219TrpTrp: 0.219 ± 0.131
0.292TrpTyr: 0.292 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.407TyrAla: 2.407 ± 0.453
0.292TyrCys: 0.292 ± 0.152
1.459TyrAsp: 1.459 ± 0.324
1.823TyrGlu: 1.823 ± 0.367
0.802TyrPhe: 0.802 ± 0.274
3.136TyrGly: 3.136 ± 0.535
0.438TyrHis: 0.438 ± 0.172
0.438TyrIle: 0.438 ± 0.19
1.021TyrLys: 1.021 ± 0.288
1.532TyrLeu: 1.532 ± 0.38
0.583TyrMet: 0.583 ± 0.178
1.021TyrAsn: 1.021 ± 0.266
1.313TyrPro: 1.313 ± 0.326
0.511TyrGln: 0.511 ± 0.206
3.282TyrArg: 3.282 ± 0.539
1.459TyrSer: 1.459 ± 0.281
0.802TyrThr: 0.802 ± 0.235
2.334TyrVal: 2.334 ± 0.336
0.583TyrTrp: 0.583 ± 0.234
1.021TyrTyr: 1.021 ± 0.29
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (13712 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski