Biostraticola tofi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Bruguierivoracaceae; Biostraticola

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3912 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3YH19|A0A4R3YH19_9GAMM Uncharacterized protein OS=Biostraticola tofi OX=466109 GN=EDC52_11720 PE=4 SV=1
MM1 pKa = 7.5AMSSNVPTEE10 pKa = 4.24LKK12 pKa = 9.44YY13 pKa = 9.08TASHH17 pKa = 5.76EE18 pKa = 4.31WVLAEE23 pKa = 4.07GNGVYY28 pKa = 10.33SVGITEE34 pKa = 4.64HH35 pKa = 6.12AQEE38 pKa = 4.67LLGDD42 pKa = 4.06MVFLDD47 pKa = 4.18LPEE50 pKa = 4.56VGTAFSAGDD59 pKa = 3.62DD60 pKa = 3.84CAVAEE65 pKa = 4.4SVKK68 pKa = 10.54AASDD72 pKa = 3.51IYY74 pKa = 11.37APISGEE80 pKa = 3.34IVAINEE86 pKa = 4.38DD87 pKa = 3.88LDD89 pKa = 4.12GSPEE93 pKa = 4.22LVNSAPYY100 pKa = 8.93TDD102 pKa = 3.37GWLFRR107 pKa = 11.84IKK109 pKa = 10.74AGDD112 pKa = 3.58EE113 pKa = 4.21TEE115 pKa = 4.23LAEE118 pKa = 4.82LLDD121 pKa = 4.02AEE123 pKa = 5.57GYY125 pKa = 10.35LATLDD130 pKa = 4.34EE131 pKa = 4.8EE132 pKa = 4.79DD133 pKa = 3.78HH134 pKa = 6.73

Molecular weight: 14.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3Z2U3|A0A4R3Z2U3_9GAMM YVTN family beta-propeller protein OS=Biostraticola tofi OX=466109 GN=EDC52_101451 PE=4 SV=1
MM1 pKa = 6.95ATARR5 pKa = 11.84SALFRR10 pKa = 11.84AGVSLKK16 pKa = 10.67GVALFLKK23 pKa = 10.43ALRR26 pKa = 11.84FFSKK30 pKa = 10.75ALRR33 pKa = 11.84FFSKK37 pKa = 10.73ALRR40 pKa = 11.84FSSSTFTLL48 pKa = 3.68

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3912

0

3912

1238576

27

2856

316.6

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.112 ± 0.048

1.047 ± 0.012

5.286 ± 0.03

5.181 ± 0.039

3.74 ± 0.027

7.577 ± 0.034

2.337 ± 0.02

5.918 ± 0.037

3.743 ± 0.033

11.246 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.02

3.491 ± 0.026

4.653 ± 0.024

4.639 ± 0.032

6.029 ± 0.037

6.193 ± 0.026

5.153 ± 0.028

6.768 ± 0.031

1.436 ± 0.019

2.788 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski