Tetrasphaera jenkinsii Ben 74

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Tetrasphaera; Tetrasphaera jenkinsii

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A077ME16|A0A077ME16_9MICO Uncharacterized protein OS=Tetrasphaera jenkinsii Ben 74 OX=1193518 GN=BN13_30045 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.68FDD5 pKa = 4.23YY6 pKa = 11.23PDD8 pKa = 3.9DD9 pKa = 3.81LRR11 pKa = 11.84YY12 pKa = 7.76TAEE15 pKa = 4.29HH16 pKa = 5.61EE17 pKa = 4.48WVRR20 pKa = 11.84SVGDD24 pKa = 3.15GRR26 pKa = 11.84VRR28 pKa = 11.84VGITAYY34 pKa = 10.48AQDD37 pKa = 3.65ALGDD41 pKa = 4.07VVFVTAPAVGDD52 pKa = 4.15SISAGDD58 pKa = 3.44ACGEE62 pKa = 4.05IEE64 pKa = 4.32STKK67 pKa = 10.64SVSDD71 pKa = 4.2LYY73 pKa = 11.6SPLTGEE79 pKa = 3.83ITAVNAALDD88 pKa = 3.87ATPEE92 pKa = 4.85LINSDD97 pKa = 3.79PYY99 pKa = 11.13GEE101 pKa = 4.48GWCFEE106 pKa = 4.11MSLADD111 pKa = 4.85AADD114 pKa = 4.44LDD116 pKa = 4.3SLMDD120 pKa = 4.04AAAYY124 pKa = 9.5RR125 pKa = 11.84DD126 pKa = 4.01SVGG129 pKa = 3.2

Molecular weight:
13.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A077M9L8|A0A077M9L8_9MICO Ribonuclease VapC OS=Tetrasphaera jenkinsii Ben 74 OX=1193518 GN=vapC PE=3 SV=1
MM1 pKa = 7.84PPTARR6 pKa = 11.84RR7 pKa = 11.84SLRR10 pKa = 11.84SQTLMFRR17 pKa = 11.84MIRR20 pKa = 11.84LAPLAIVRR28 pKa = 11.84GFAPRR33 pKa = 11.84MPPKK37 pKa = 10.15ARR39 pKa = 11.84RR40 pKa = 11.84SLRR43 pKa = 11.84SQTLMFRR50 pKa = 11.84TIRR53 pKa = 11.84LAPLAIVRR61 pKa = 11.84RR62 pKa = 11.84FAIVPGTPQPSSRR75 pKa = 11.84RR76 pKa = 11.84WTTSSS81 pKa = 2.95

Molecular weight:
9.29 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3715

0

3715

1123227

11

2043

302.3

32.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.612 ± 0.063

0.76 ± 0.01

6.202 ± 0.036

5.162 ± 0.037

2.818 ± 0.025

9.158 ± 0.04

2.156 ± 0.023

4.114 ± 0.03

2.145 ± 0.033

9.987 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.95 ± 0.018

1.942 ± 0.026

5.78 ± 0.033

2.73 ± 0.021

7.687 ± 0.052

5.524 ± 0.035

6.157 ± 0.037

8.586 ± 0.043

1.57 ± 0.019

1.959 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski