Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium sambucinum species complex; Fusarium graminearum

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14163 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C3YHX9|A0A1C3YHX9_GIBZE Uncharacterized protein OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=FGRAMPH1_01T02241 PE=4 SV=1
MM1 pKa = 7.49LLVFLLNASFYY12 pKa = 9.98SLRR15 pKa = 11.84LTAYY19 pKa = 9.2RR20 pKa = 11.84VASTTMISSWQLAAVLLLAGPSLADD45 pKa = 3.56NFTISNGQIFTPGFVVLDD63 pKa = 3.94APQPYY68 pKa = 8.8TPLGGDD74 pKa = 3.74TLHH77 pKa = 6.75VAIDD81 pKa = 3.34VTANGKK87 pKa = 9.68LPLPSNDD94 pKa = 5.21DD95 pKa = 4.79DD96 pKa = 6.04NDD98 pKa = 3.82DD99 pKa = 4.42DD100 pKa = 4.21NQIFSIEE107 pKa = 4.11MFLYY111 pKa = 10.45SYY113 pKa = 9.46VTGRR117 pKa = 11.84NFTISNGTASANNASLGEE135 pKa = 4.07IMAQEE140 pKa = 4.25PGSTVKK146 pKa = 10.33HH147 pKa = 5.91VNWIWPEE154 pKa = 3.92CLVGDD159 pKa = 4.76GAPEE163 pKa = 4.43GDD165 pKa = 3.88SDD167 pKa = 3.95RR168 pKa = 11.84GVYY171 pKa = 10.23NISIRR176 pKa = 11.84QNFRR180 pKa = 11.84YY181 pKa = 10.47NGDD184 pKa = 3.36DD185 pKa = 3.84YY186 pKa = 11.53YY187 pKa = 11.16TIFDD191 pKa = 3.78VPISVNNSIPEE202 pKa = 4.33GNDD205 pKa = 3.04RR206 pKa = 11.84PSCDD210 pKa = 3.21GLSNEE215 pKa = 4.49ILSPEE220 pKa = 4.77DD221 pKa = 3.33IDD223 pKa = 4.75VEE225 pKa = 4.16AANEE229 pKa = 4.17VGVLFAPGDD238 pKa = 3.74ATEE241 pKa = 4.79LDD243 pKa = 3.31ISGEE247 pKa = 3.99NSVGSVFGPSMRR259 pKa = 11.84LYY261 pKa = 9.77VGVVGVFMALLVV273 pKa = 3.75

Molecular weight: 29.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1S0M1|I1S0M1_GIBZE Uncharacterized protein OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=FGRAMPH1_01T07657 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APARR26 pKa = 11.84SNRR29 pKa = 11.84HH30 pKa = 4.32TVTTTTTTTTKK41 pKa = 9.91PRR43 pKa = 11.84RR44 pKa = 11.84GMFGGGSTARR54 pKa = 11.84RR55 pKa = 11.84THH57 pKa = 5.96GAAPVHH63 pKa = 5.35HH64 pKa = 6.52HH65 pKa = 5.47QRR67 pKa = 11.84RR68 pKa = 11.84PSMKK72 pKa = 10.02DD73 pKa = 2.95KK74 pKa = 11.41VSGALLKK81 pKa = 11.04LKK83 pKa = 10.68GSLTRR88 pKa = 11.84RR89 pKa = 11.84PGVKK93 pKa = 9.89AAGTRR98 pKa = 11.84RR99 pKa = 11.84MHH101 pKa = 5.65GTDD104 pKa = 2.99GRR106 pKa = 11.84GARR109 pKa = 11.84HH110 pKa = 5.87HH111 pKa = 7.23RR112 pKa = 11.84YY113 pKa = 9.44

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14162

1

14163

6396450

15

11197

451.6

50.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.21 ± 0.018

1.325 ± 0.008

5.905 ± 0.017

6.174 ± 0.021

3.749 ± 0.014

6.705 ± 0.02

2.383 ± 0.009

5.102 ± 0.015

5.108 ± 0.017

8.668 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.009

3.867 ± 0.012

5.892 ± 0.025

4.047 ± 0.017

5.807 ± 0.02

8.204 ± 0.023

6.123 ± 0.027

6.13 ± 0.016

1.514 ± 0.008

2.795 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski