Sporobolus striate mosaic virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7FGY6|J7FGY6_9GEMI Capsid protein OS=Sporobolus striate mosaic virus 1 OX=1302849 PE=3 SV=1
MM1 pKa = 7.58SGPSRR6 pKa = 11.84PPSPFAISSSDD17 pKa = 3.57EE18 pKa = 3.86EE19 pKa = 4.73SVDD22 pKa = 3.39GFHH25 pKa = 7.52FRR27 pKa = 11.84GKK29 pKa = 10.35NIFLTYY35 pKa = 9.85SRR37 pKa = 11.84CEE39 pKa = 3.75IDD41 pKa = 3.31PALITDD47 pKa = 4.45ALWDD51 pKa = 3.85KK52 pKa = 10.88FSSHH56 pKa = 6.44KK57 pKa = 9.86PLYY60 pKa = 8.84ILSVRR65 pKa = 11.84EE66 pKa = 3.75LHH68 pKa = 6.12QDD70 pKa = 2.88SGFHH74 pKa = 4.54VHH76 pKa = 6.95CLVQLTDD83 pKa = 3.44QYY85 pKa = 11.42RR86 pKa = 11.84SRR88 pKa = 11.84DD89 pKa = 3.35SSFADD94 pKa = 3.86LGGNHH99 pKa = 7.05PNIQTVRR106 pKa = 11.84SATKK110 pKa = 9.1VKK112 pKa = 10.33EE113 pKa = 4.27YY114 pKa = 10.36ILKK117 pKa = 10.53EE118 pKa = 3.96PVSQSARR125 pKa = 11.84GKK127 pKa = 9.65FVAPGGRR134 pKa = 11.84PPKK137 pKa = 9.23HH138 pKa = 5.48TDD140 pKa = 2.61RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84SDD145 pKa = 3.34SAVKK149 pKa = 10.12DD150 pKa = 3.2EE151 pKa = 3.94RR152 pKa = 11.84MRR154 pKa = 11.84YY155 pKa = 8.78ILRR158 pKa = 11.84TATTRR163 pKa = 11.84DD164 pKa = 3.68DD165 pKa = 3.7YY166 pKa = 11.77LGMVRR171 pKa = 11.84KK172 pKa = 10.03SFPFEE177 pKa = 3.34WATRR181 pKa = 11.84LAQFEE186 pKa = 4.38YY187 pKa = 10.43SASKK191 pKa = 10.39LFPDD195 pKa = 4.37ITPQYY200 pKa = 9.08QSQYY204 pKa = 7.53QTTDD208 pKa = 3.34LTCHH212 pKa = 6.28EE213 pKa = 5.28NLLDD217 pKa = 4.03WYY219 pKa = 10.65QEE221 pKa = 3.84NLQCYY226 pKa = 8.27IVSPFAYY233 pKa = 9.77SCLHH237 pKa = 6.07PAEE240 pKa = 4.96DD241 pKa = 5.08AEE243 pKa = 5.93SDD245 pKa = 4.31LKK247 pKa = 11.08WMADD251 pKa = 3.39VTRR254 pKa = 11.84TEE256 pKa = 3.97QGAGNPSTSADD267 pKa = 3.19QLVPVRR273 pKa = 11.84NLGPGCC279 pKa = 4.02

Molecular weight: 31.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7FHN0|J7FHN0_9GEMI Movement protein OS=Sporobolus striate mosaic virus 1 OX=1302849 PE=3 SV=1
MM1 pKa = 7.5EE2 pKa = 6.04AGHH5 pKa = 7.11LPSQQGFPSPLAYY18 pKa = 10.28SQPSPSGVGNDD29 pKa = 3.76SAWRR33 pKa = 11.84TLVLVFTITAVGLACSFALYY53 pKa = 10.0RR54 pKa = 11.84LCVKK58 pKa = 10.71DD59 pKa = 4.79LVLLLRR65 pKa = 11.84AKK67 pKa = 10.48RR68 pKa = 11.84SRR70 pKa = 11.84TVTEE74 pKa = 4.4LGFGGTPARR83 pKa = 11.84QDD85 pKa = 3.35GVRR88 pKa = 11.84TGSGVPGLGG97 pKa = 3.22

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

963

97

375

240.8

27.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.438 ± 0.599

1.765 ± 0.369

6.231 ± 0.911

4.154 ± 0.501

4.777 ± 0.156

6.646 ± 1.316

2.7 ± 0.329

4.154 ± 0.677

4.984 ± 0.657

7.892 ± 0.884

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.765 ± 0.202

3.115 ± 0.504

6.231 ± 0.294

4.673 ± 0.312

7.061 ± 0.201

8.307 ± 0.916

5.815 ± 0.269

6.646 ± 1.463

1.765 ± 0.202

4.881 ± 0.549
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski