Apis mellifera associated microvirus 33

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTU5|A0A3S8UTU5_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 33 OX=2494762 PE=4 SV=1
MM1 pKa = 7.35AHH3 pKa = 7.02LRR5 pKa = 11.84TDD7 pKa = 3.66TASNAHH13 pKa = 6.65FSRR16 pKa = 11.84VPTISRR22 pKa = 11.84SRR24 pKa = 11.84NAFSVAEE31 pKa = 4.04KK32 pKa = 10.51HH33 pKa = 4.9VTTSQFDD40 pKa = 3.77YY41 pKa = 10.84LYY43 pKa = 9.77PLYY46 pKa = 9.89WKK48 pKa = 10.23YY49 pKa = 10.7IYY51 pKa = 10.44PGDD54 pKa = 4.32TISLTHH60 pKa = 6.51GLLARR65 pKa = 11.84LQTQIAVLYY74 pKa = 10.28DD75 pKa = 3.59DD76 pKa = 5.99LYY78 pKa = 11.71VDD80 pKa = 3.31LHH82 pKa = 6.78AWFVPFRR89 pKa = 11.84LLQTNWARR97 pKa = 11.84YY98 pKa = 7.43QFNAQPLGPSQDD110 pKa = 3.49NSSLTSPKK118 pKa = 8.89ITLSGLGAGGFTAKK132 pKa = 10.21SLYY135 pKa = 10.39DD136 pKa = 3.43YY137 pKa = 10.99FGFPTEE143 pKa = 4.54INLSADD149 pKa = 3.49TQHH152 pKa = 6.45INNYY156 pKa = 9.69LGRR159 pKa = 11.84AYY161 pKa = 11.11NLIWNEE167 pKa = 3.67NYY169 pKa = 10.0RR170 pKa = 11.84DD171 pKa = 3.74QNLQNPATVDD181 pKa = 3.93LDD183 pKa = 4.42DD184 pKa = 6.26GPDD187 pKa = 3.4NPADD191 pKa = 3.69YY192 pKa = 10.74ILRR195 pKa = 11.84KK196 pKa = 9.23RR197 pKa = 11.84GKK199 pKa = 8.6RR200 pKa = 11.84HH201 pKa = 6.84DD202 pKa = 4.15RR203 pKa = 11.84FTSALTSQQKK213 pKa = 8.05GTAVTIPLGTSAPVLGIGTNTVQSWTAGPLTVNEE247 pKa = 4.35SDD249 pKa = 3.71LTTSQYY255 pKa = 11.86ANYY258 pKa = 10.35LLFRR262 pKa = 11.84GGSSDD267 pKa = 4.34IYY269 pKa = 11.01LEE271 pKa = 4.18KK272 pKa = 10.61QGSNNAPNIRR282 pKa = 11.84ADD284 pKa = 3.72LTGATAATLNQLRR297 pKa = 11.84QSVAVQHH304 pKa = 6.46LLEE307 pKa = 5.35ADD309 pKa = 3.31ARR311 pKa = 11.84GGTRR315 pKa = 11.84DD316 pKa = 3.03VEE318 pKa = 4.66AIHH321 pKa = 6.16NRR323 pKa = 11.84WGVVVPDD330 pKa = 4.74FRR332 pKa = 11.84LQRR335 pKa = 11.84PEE337 pKa = 3.83YY338 pKa = 10.63LGGQTFTFDD347 pKa = 3.29GHH349 pKa = 6.22VVPQTSATEE358 pKa = 4.1VGGTPQANLTQFSQAMTHH376 pKa = 6.27FNVNHH381 pKa = 6.28SFVEE385 pKa = 4.02HH386 pKa = 5.61GVYY389 pKa = 8.93MVLASARR396 pKa = 11.84SNITYY401 pKa = 9.62QQGLLRR407 pKa = 11.84EE408 pKa = 4.19LSYY411 pKa = 9.95RR412 pKa = 11.84TRR414 pKa = 11.84YY415 pKa = 10.11DD416 pKa = 2.65WFQPEE421 pKa = 4.13FANLGEE427 pKa = 4.09VAIKK431 pKa = 10.72NKK433 pKa = 9.53EE434 pKa = 3.62IYY436 pKa = 9.44MDD438 pKa = 4.3GSSADD443 pKa = 3.45EE444 pKa = 4.24STFGYY449 pKa = 9.96QEE451 pKa = 3.6YY452 pKa = 9.95AYY454 pKa = 9.72EE455 pKa = 4.46LRR457 pKa = 11.84YY458 pKa = 10.77SMNRR462 pKa = 11.84VTAEE466 pKa = 3.58MRR468 pKa = 11.84SNYY471 pKa = 8.05ATSLDD476 pKa = 4.02SKK478 pKa = 11.16HH479 pKa = 6.25MSDD482 pKa = 5.0EE483 pKa = 4.31YY484 pKa = 11.65ASLPTLGSAWIQSDD498 pKa = 3.97TPISRR503 pKa = 11.84NIAVSAATADD513 pKa = 4.25PIEE516 pKa = 4.74INTMAKK522 pKa = 10.37GMIARR527 pKa = 11.84TLPMYY532 pKa = 10.13SVPGLMRR539 pKa = 11.84LL540 pKa = 3.55

Molecular weight: 60.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTT5|A0A3S8UTT5_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 33 OX=2494762 PE=3 SV=1
MM1 pKa = 7.65EE2 pKa = 5.85KK3 pKa = 10.66GFSHH7 pKa = 6.61VKK9 pKa = 10.56CFDD12 pKa = 3.28PTLCYY17 pKa = 9.84TDD19 pKa = 3.96SRR21 pKa = 11.84GKK23 pKa = 10.01KK24 pKa = 9.14HH25 pKa = 5.66FRR27 pKa = 11.84HH28 pKa = 5.94YY29 pKa = 11.36SLANNLFLQMAQQVFDD45 pKa = 3.85CGKK48 pKa = 10.23CLRR51 pKa = 11.84CRR53 pKa = 11.84KK54 pKa = 9.83AKK56 pKa = 10.63ANEE59 pKa = 3.53LAARR63 pKa = 11.84CVLHH67 pKa = 7.88ASMYY71 pKa = 10.8DD72 pKa = 3.59SNCFLTLTYY81 pKa = 10.5DD82 pKa = 3.63EE83 pKa = 5.03KK84 pKa = 11.46KK85 pKa = 10.55EE86 pKa = 4.19GYY88 pKa = 9.68HH89 pKa = 6.89NNFNYY94 pKa = 10.63EE95 pKa = 4.03DD96 pKa = 3.53VQKK99 pKa = 10.78FKK101 pKa = 11.08KK102 pKa = 10.07RR103 pKa = 11.84LRR105 pKa = 11.84RR106 pKa = 11.84WVQYY110 pKa = 10.41HH111 pKa = 4.85FNKK114 pKa = 10.34RR115 pKa = 11.84IEE117 pKa = 4.02IFNVHH122 pKa = 6.48EE123 pKa = 4.0YY124 pKa = 10.84GKK126 pKa = 10.14NGKK129 pKa = 8.28KK130 pKa = 9.64HH131 pKa = 4.32YY132 pKa = 10.18HH133 pKa = 6.25LIVFNFDD140 pKa = 3.53FLDD143 pKa = 3.99KK144 pKa = 11.11EE145 pKa = 4.22IFTKK149 pKa = 10.87KK150 pKa = 10.36NGLPLYY156 pKa = 9.19TSKK159 pKa = 10.63ILEE162 pKa = 4.34KK163 pKa = 9.84RR164 pKa = 11.84WSHH167 pKa = 5.51GFCTIGDD174 pKa = 3.79VSEE177 pKa = 4.44ASAMYY182 pKa = 8.62QAKK185 pKa = 10.38YY186 pKa = 9.05MEE188 pKa = 5.28KK189 pKa = 10.25DD190 pKa = 3.55FQHH193 pKa = 6.88GNVTNSKK200 pKa = 9.84KK201 pKa = 10.33SHH203 pKa = 5.4SQHH206 pKa = 6.85SGIGRR211 pKa = 11.84PYY213 pKa = 8.95FLKK216 pKa = 10.47HH217 pKa = 5.3FRR219 pKa = 11.84QILSLGFIPFNGKK232 pKa = 9.43QIPVPRR238 pKa = 11.84YY239 pKa = 9.18FEE241 pKa = 4.26KK242 pKa = 10.63LAHH245 pKa = 6.62KK246 pKa = 9.92RR247 pKa = 11.84WCHH250 pKa = 5.53YY251 pKa = 10.06NDD253 pKa = 3.56PSAFVDD259 pKa = 4.52LPSRR263 pKa = 11.84DD264 pKa = 3.69ALYY267 pKa = 10.05TPFKK271 pKa = 10.01TGPLPYY277 pKa = 10.05SQLEE281 pKa = 3.88MSNLYY286 pKa = 9.3EE287 pKa = 4.1QYY289 pKa = 9.12KK290 pKa = 8.13TNKK293 pKa = 9.18DD294 pKa = 3.18AKK296 pKa = 8.19TQEE299 pKa = 4.62LAQEE303 pKa = 4.29WKK305 pKa = 10.95NVIQEE310 pKa = 4.09HH311 pKa = 6.84LKK313 pKa = 9.93TKK315 pKa = 10.22IPPDD319 pKa = 3.5FRR321 pKa = 11.84KK322 pKa = 10.4AGSNVLYY329 pKa = 10.43DD330 pKa = 3.84LKK332 pKa = 11.2NRR334 pKa = 11.84NNQEE338 pKa = 3.81KK339 pKa = 10.09FF340 pKa = 3.21

Molecular weight: 40.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1370

104

540

274.0

31.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.394 ± 1.173

0.73 ± 0.587

5.109 ± 0.277

5.474 ± 1.087

4.599 ± 0.917

6.131 ± 0.888

3.139 ± 0.637

4.453 ± 0.551

6.715 ± 2.228

8.832 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.847 ± 0.612

6.204 ± 0.38

4.234 ± 0.233

5.328 ± 0.221

4.891 ± 0.455

6.642 ± 1.261

6.423 ± 1.051

4.234 ± 0.428

0.876 ± 0.341

4.745 ± 0.729
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski