Amino acid dipepetide frequency for Pseudomonas phage MR6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.504AlaAla: 13.504 ± 1.175
0.97AlaCys: 0.97 ± 0.224
6.79AlaAsp: 6.79 ± 0.679
6.715AlaGlu: 6.715 ± 0.836
2.761AlaPhe: 2.761 ± 0.543
9.475AlaGly: 9.475 ± 1.048
1.641AlaHis: 1.641 ± 0.393
3.731AlaIle: 3.731 ± 0.439
5.67AlaLys: 5.67 ± 0.549
10.669AlaLeu: 10.669 ± 0.963
4.327AlaMet: 4.327 ± 0.646
4.029AlaAsn: 4.029 ± 0.545
3.954AlaPro: 3.954 ± 0.621
5.297AlaGln: 5.297 ± 0.704
5.82AlaArg: 5.82 ± 0.659
5.82AlaSer: 5.82 ± 0.652
6.043AlaThr: 6.043 ± 0.905
7.536AlaVal: 7.536 ± 0.759
1.045AlaTrp: 1.045 ± 0.266
3.581AlaTyr: 3.581 ± 0.53
0.0AlaXaa: 0.0 ± 0.0
Cys
1.268CysAla: 1.268 ± 0.313
0.373CysCys: 0.373 ± 0.161
0.671CysAsp: 0.671 ± 0.241
0.671CysGlu: 0.671 ± 0.241
0.298CysPhe: 0.298 ± 0.127
1.119CysGly: 1.119 ± 0.301
0.373CysHis: 0.373 ± 0.168
0.298CysIle: 0.298 ± 0.162
0.373CysLys: 0.373 ± 0.138
1.268CysLeu: 1.268 ± 0.322
0.373CysMet: 0.373 ± 0.169
0.597CysAsn: 0.597 ± 0.226
0.597CysPro: 0.597 ± 0.2
0.448CysGln: 0.448 ± 0.139
0.97CysArg: 0.97 ± 0.299
0.97CysSer: 0.97 ± 0.342
1.045CysThr: 1.045 ± 0.276
0.97CysVal: 0.97 ± 0.281
0.224CysTrp: 0.224 ± 0.122
0.522CysTyr: 0.522 ± 0.215
0.0CysXaa: 0.0 ± 0.0
Asp
5.148AspAla: 5.148 ± 0.709
0.448AspCys: 0.448 ± 0.174
2.984AspAsp: 2.984 ± 0.369
2.984AspGlu: 2.984 ± 0.459
2.089AspPhe: 2.089 ± 0.437
3.954AspGly: 3.954 ± 0.569
0.746AspHis: 0.746 ± 0.222
3.731AspIle: 3.731 ± 0.551
2.761AspLys: 2.761 ± 0.501
3.88AspLeu: 3.88 ± 0.487
2.537AspMet: 2.537 ± 0.336
2.238AspAsn: 2.238 ± 0.458
2.91AspPro: 2.91 ± 0.501
1.641AspGln: 1.641 ± 0.342
3.581AspArg: 3.581 ± 0.521
3.656AspSer: 3.656 ± 0.451
3.88AspThr: 3.88 ± 0.415
4.253AspVal: 4.253 ± 0.56
0.448AspTrp: 0.448 ± 0.151
2.164AspTyr: 2.164 ± 0.389
0.0AspXaa: 0.0 ± 0.0
Glu
7.386GluAla: 7.386 ± 0.869
0.597GluCys: 0.597 ± 0.24
2.835GluAsp: 2.835 ± 0.354
2.835GluGlu: 2.835 ± 0.408
1.791GluPhe: 1.791 ± 0.388
2.537GluGly: 2.537 ± 0.44
1.194GluHis: 1.194 ± 0.278
1.492GluIle: 1.492 ± 0.265
2.388GluLys: 2.388 ± 0.482
6.043GluLeu: 6.043 ± 0.719
1.567GluMet: 1.567 ± 0.402
1.418GluAsn: 1.418 ± 0.279
0.97GluPro: 0.97 ± 0.242
3.805GluGln: 3.805 ± 0.453
3.581GluArg: 3.581 ± 0.576
2.91GluSer: 2.91 ± 0.438
2.537GluThr: 2.537 ± 0.365
3.507GluVal: 3.507 ± 0.562
0.895GluTrp: 0.895 ± 0.239
2.313GluTyr: 2.313 ± 0.324
0.0GluXaa: 0.0 ± 0.0
Phe
2.537PheAla: 2.537 ± 0.355
0.597PheCys: 0.597 ± 0.268
2.089PheAsp: 2.089 ± 0.383
1.492PheGlu: 1.492 ± 0.365
0.895PhePhe: 0.895 ± 0.235
2.537PheGly: 2.537 ± 0.345
0.671PheHis: 0.671 ± 0.227
1.268PheIle: 1.268 ± 0.246
1.492PheLys: 1.492 ± 0.366
3.059PheLeu: 3.059 ± 0.527
0.821PheMet: 0.821 ± 0.267
1.641PheAsn: 1.641 ± 0.424
1.268PhePro: 1.268 ± 0.265
0.821PheGln: 0.821 ± 0.26
2.014PheArg: 2.014 ± 0.44
2.164PheSer: 2.164 ± 0.326
2.164PheThr: 2.164 ± 0.333
2.238PheVal: 2.238 ± 0.344
0.298PheTrp: 0.298 ± 0.191
0.448PheTyr: 0.448 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
7.834GlyAla: 7.834 ± 0.839
1.492GlyCys: 1.492 ± 0.386
4.477GlyAsp: 4.477 ± 0.568
3.581GlyGlu: 3.581 ± 0.481
2.537GlyPhe: 2.537 ± 0.66
6.939GlyGly: 6.939 ± 0.733
0.671GlyHis: 0.671 ± 0.201
3.059GlyIle: 3.059 ± 0.531
4.402GlyLys: 4.402 ± 0.603
7.237GlyLeu: 7.237 ± 0.824
2.611GlyMet: 2.611 ± 0.368
2.686GlyAsn: 2.686 ± 0.323
2.611GlyPro: 2.611 ± 0.465
3.805GlyGln: 3.805 ± 0.901
3.731GlyArg: 3.731 ± 0.417
4.253GlySer: 4.253 ± 0.52
5.148GlyThr: 5.148 ± 0.591
7.909GlyVal: 7.909 ± 0.907
1.418GlyTrp: 1.418 ± 0.316
3.134GlyTyr: 3.134 ± 0.361
0.0GlyXaa: 0.0 ± 0.0
His
1.94HisAla: 1.94 ± 0.386
0.298HisCys: 0.298 ± 0.187
1.791HisAsp: 1.791 ± 0.414
1.045HisGlu: 1.045 ± 0.262
0.746HisPhe: 0.746 ± 0.211
1.716HisGly: 1.716 ± 0.414
0.373HisHis: 0.373 ± 0.163
0.97HisIle: 0.97 ± 0.257
1.791HisLys: 1.791 ± 0.316
1.194HisLeu: 1.194 ± 0.359
0.597HisMet: 0.597 ± 0.241
0.597HisAsn: 0.597 ± 0.218
0.895HisPro: 0.895 ± 0.276
0.671HisGln: 0.671 ± 0.187
1.492HisArg: 1.492 ± 0.355
0.746HisSer: 0.746 ± 0.225
0.821HisThr: 0.821 ± 0.24
1.343HisVal: 1.343 ± 0.347
0.448HisTrp: 0.448 ± 0.168
0.597HisTyr: 0.597 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
4.029IleAla: 4.029 ± 0.591
0.373IleCys: 0.373 ± 0.151
2.313IleAsp: 2.313 ± 0.398
2.761IleGlu: 2.761 ± 0.384
0.522IlePhe: 0.522 ± 0.198
3.581IleGly: 3.581 ± 0.433
1.119IleHis: 1.119 ± 0.234
2.313IleIle: 2.313 ± 0.336
2.611IleLys: 2.611 ± 0.45
2.761IleLeu: 2.761 ± 0.482
0.97IleMet: 0.97 ± 0.217
1.791IleAsn: 1.791 ± 0.393
2.761IlePro: 2.761 ± 0.436
2.611IleGln: 2.611 ± 0.332
3.134IleArg: 3.134 ± 0.394
1.492IleSer: 1.492 ± 0.376
2.537IleThr: 2.537 ± 0.48
2.761IleVal: 2.761 ± 0.52
0.298IleTrp: 0.298 ± 0.143
0.597IleTyr: 0.597 ± 0.177
0.0IleXaa: 0.0 ± 0.0
Lys
5.297LysAla: 5.297 ± 0.899
0.597LysCys: 0.597 ± 0.187
2.462LysAsp: 2.462 ± 0.325
2.238LysGlu: 2.238 ± 0.469
1.716LysPhe: 1.716 ± 0.328
2.984LysGly: 2.984 ± 0.516
1.119LysHis: 1.119 ± 0.29
2.014LysIle: 2.014 ± 0.383
1.119LysLys: 1.119 ± 0.324
4.178LysLeu: 4.178 ± 0.533
0.821LysMet: 0.821 ± 0.19
1.194LysAsn: 1.194 ± 0.293
2.164LysPro: 2.164 ± 0.441
2.388LysGln: 2.388 ± 0.36
2.537LysArg: 2.537 ± 0.43
2.164LysSer: 2.164 ± 0.54
2.835LysThr: 2.835 ± 0.388
4.178LysVal: 4.178 ± 0.686
0.597LysTrp: 0.597 ± 0.205
1.268LysTyr: 1.268 ± 0.347
0.0LysXaa: 0.0 ± 0.0
Leu
9.625LeuAla: 9.625 ± 0.863
1.418LeuCys: 1.418 ± 0.337
6.043LeuAsp: 6.043 ± 0.68
5.372LeuGlu: 5.372 ± 0.57
2.089LeuPhe: 2.089 ± 0.512
7.61LeuGly: 7.61 ± 0.624
2.388LeuHis: 2.388 ± 0.431
2.984LeuIle: 2.984 ± 0.497
2.835LeuLys: 2.835 ± 0.489
6.566LeuLeu: 6.566 ± 0.8
2.388LeuMet: 2.388 ± 0.291
4.327LeuAsn: 4.327 ± 0.782
4.327LeuPro: 4.327 ± 0.672
3.507LeuGln: 3.507 ± 0.46
5.894LeuArg: 5.894 ± 0.692
6.267LeuSer: 6.267 ± 0.652
5.596LeuThr: 5.596 ± 0.739
6.416LeuVal: 6.416 ± 0.823
1.268LeuTrp: 1.268 ± 0.353
2.238LeuTyr: 2.238 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
3.208MetAla: 3.208 ± 0.602
0.224MetCys: 0.224 ± 0.132
1.418MetAsp: 1.418 ± 0.453
1.418MetGlu: 1.418 ± 0.338
1.119MetPhe: 1.119 ± 0.21
1.791MetGly: 1.791 ± 0.244
0.448MetHis: 0.448 ± 0.148
0.821MetIle: 0.821 ± 0.241
1.045MetLys: 1.045 ± 0.346
2.761MetLeu: 2.761 ± 0.32
0.597MetMet: 0.597 ± 0.195
1.119MetAsn: 1.119 ± 0.285
1.194MetPro: 1.194 ± 0.27
2.313MetGln: 2.313 ± 0.518
1.94MetArg: 1.94 ± 0.349
2.238MetSer: 2.238 ± 0.375
2.164MetThr: 2.164 ± 0.341
2.089MetVal: 2.089 ± 0.351
0.597MetTrp: 0.597 ± 0.221
1.119MetTyr: 1.119 ± 0.31
0.0MetXaa: 0.0 ± 0.0
Asn
4.029AsnAla: 4.029 ± 0.705
0.671AsnCys: 0.671 ± 0.207
1.791AsnAsp: 1.791 ± 0.37
2.313AsnGlu: 2.313 ± 0.338
0.895AsnPhe: 0.895 ± 0.338
3.507AsnGly: 3.507 ± 0.546
0.97AsnHis: 0.97 ± 0.308
1.492AsnIle: 1.492 ± 0.344
1.418AsnLys: 1.418 ± 0.359
3.581AsnLeu: 3.581 ± 0.412
1.268AsnMet: 1.268 ± 0.303
1.119AsnAsn: 1.119 ± 0.287
2.313AsnPro: 2.313 ± 0.295
1.567AsnGln: 1.567 ± 0.548
2.014AsnArg: 2.014 ± 0.495
2.462AsnSer: 2.462 ± 0.581
2.164AsnThr: 2.164 ± 0.489
3.581AsnVal: 3.581 ± 0.468
0.671AsnTrp: 0.671 ± 0.209
1.418AsnTyr: 1.418 ± 0.297
0.0AsnXaa: 0.0 ± 0.0
Pro
5.82ProAla: 5.82 ± 0.733
0.448ProCys: 0.448 ± 0.2
1.791ProAsp: 1.791 ± 0.311
2.984ProGlu: 2.984 ± 0.575
1.119ProPhe: 1.119 ± 0.233
3.656ProGly: 3.656 ± 0.47
0.671ProHis: 0.671 ± 0.224
1.865ProIle: 1.865 ± 0.39
1.865ProLys: 1.865 ± 0.456
2.835ProLeu: 2.835 ± 0.401
0.597ProMet: 0.597 ± 0.197
2.089ProAsn: 2.089 ± 0.322
1.268ProPro: 1.268 ± 0.319
1.791ProGln: 1.791 ± 0.314
2.462ProArg: 2.462 ± 0.381
2.984ProSer: 2.984 ± 0.446
2.238ProThr: 2.238 ± 0.516
3.656ProVal: 3.656 ± 0.583
1.045ProTrp: 1.045 ± 0.277
1.94ProTyr: 1.94 ± 0.348
0.0ProXaa: 0.0 ± 0.0
Gln
5.894GlnAla: 5.894 ± 1.0
0.448GlnCys: 0.448 ± 0.178
1.716GlnAsp: 1.716 ± 0.406
2.462GlnGlu: 2.462 ± 0.662
1.716GlnPhe: 1.716 ± 0.324
3.954GlnGly: 3.954 ± 0.55
1.343GlnHis: 1.343 ± 0.329
1.492GlnIle: 1.492 ± 0.37
1.567GlnLys: 1.567 ± 0.298
5.223GlnLeu: 5.223 ± 0.546
1.268GlnMet: 1.268 ± 0.341
1.865GlnAsn: 1.865 ± 0.242
1.567GlnPro: 1.567 ± 0.297
3.283GlnGln: 3.283 ± 0.492
3.581GlnArg: 3.581 ± 0.412
2.611GlnSer: 2.611 ± 0.455
2.164GlnThr: 2.164 ± 0.415
2.313GlnVal: 2.313 ± 0.448
0.597GlnTrp: 0.597 ± 0.207
1.716GlnTyr: 1.716 ± 0.362
0.0GlnXaa: 0.0 ± 0.0
Arg
5.67ArgAla: 5.67 ± 0.549
0.895ArgCys: 0.895 ± 0.243
4.178ArgAsp: 4.178 ± 0.404
4.178ArgGlu: 4.178 ± 0.716
2.164ArgPhe: 2.164 ± 0.359
4.551ArgGly: 4.551 ± 0.554
1.865ArgHis: 1.865 ± 0.386
3.283ArgIle: 3.283 ± 0.494
2.611ArgLys: 2.611 ± 0.479
5.67ArgLeu: 5.67 ± 0.672
2.686ArgMet: 2.686 ± 0.463
1.94ArgAsn: 1.94 ± 0.328
1.567ArgPro: 1.567 ± 0.452
2.164ArgGln: 2.164 ± 0.422
4.327ArgArg: 4.327 ± 0.54
3.731ArgSer: 3.731 ± 0.564
2.388ArgThr: 2.388 ± 0.397
4.551ArgVal: 4.551 ± 0.46
1.045ArgTrp: 1.045 ± 0.259
2.238ArgTyr: 2.238 ± 0.507
0.0ArgXaa: 0.0 ± 0.0
Ser
6.864SerAla: 6.864 ± 0.59
1.045SerCys: 1.045 ± 0.315
3.134SerAsp: 3.134 ± 0.455
2.537SerGlu: 2.537 ± 0.416
2.686SerPhe: 2.686 ± 0.455
4.477SerGly: 4.477 ± 0.52
0.97SerHis: 0.97 ± 0.253
3.507SerIle: 3.507 ± 0.5
3.059SerLys: 3.059 ± 0.632
5.521SerLeu: 5.521 ± 0.651
2.238SerMet: 2.238 ± 0.41
2.388SerAsn: 2.388 ± 0.421
2.537SerPro: 2.537 ± 0.515
2.014SerGln: 2.014 ± 0.426
2.835SerArg: 2.835 ± 0.52
3.954SerSer: 3.954 ± 0.685
3.805SerThr: 3.805 ± 0.582
4.327SerVal: 4.327 ± 0.668
1.641SerTrp: 1.641 ± 0.308
1.492SerTyr: 1.492 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
6.79ThrAla: 6.79 ± 1.058
0.746ThrCys: 0.746 ± 0.276
2.761ThrAsp: 2.761 ± 0.41
1.641ThrGlu: 1.641 ± 0.383
1.865ThrPhe: 1.865 ± 0.293
6.79ThrGly: 6.79 ± 0.864
0.821ThrHis: 0.821 ± 0.234
2.388ThrIle: 2.388 ± 0.443
2.238ThrLys: 2.238 ± 0.353
4.7ThrLeu: 4.7 ± 0.661
0.895ThrMet: 0.895 ± 0.242
2.313ThrAsn: 2.313 ± 0.463
4.029ThrPro: 4.029 ± 0.686
3.134ThrGln: 3.134 ± 0.491
2.91ThrArg: 2.91 ± 0.427
3.88ThrSer: 3.88 ± 0.497
4.104ThrThr: 4.104 ± 0.818
4.477ThrVal: 4.477 ± 0.743
0.597ThrTrp: 0.597 ± 0.198
2.462ThrTyr: 2.462 ± 0.402
0.0ThrXaa: 0.0 ± 0.0
Val
8.058ValAla: 8.058 ± 0.916
0.895ValCys: 0.895 ± 0.259
5.372ValAsp: 5.372 ± 0.643
3.507ValGlu: 3.507 ± 0.549
2.014ValPhe: 2.014 ± 0.543
5.297ValGly: 5.297 ± 0.587
1.716ValHis: 1.716 ± 0.499
2.164ValIle: 2.164 ± 0.385
2.835ValLys: 2.835 ± 0.455
7.685ValLeu: 7.685 ± 0.803
2.089ValMet: 2.089 ± 0.405
3.507ValAsn: 3.507 ± 0.79
3.731ValPro: 3.731 ± 0.589
3.134ValGln: 3.134 ± 0.555
5.148ValArg: 5.148 ± 0.7
5.372ValSer: 5.372 ± 0.884
4.327ValThr: 4.327 ± 0.874
4.999ValVal: 4.999 ± 0.743
1.119ValTrp: 1.119 ± 0.221
2.089ValTyr: 2.089 ± 0.379
0.0ValXaa: 0.0 ± 0.0
Trp
2.164TrpAla: 2.164 ± 0.402
0.522TrpCys: 0.522 ± 0.212
0.597TrpAsp: 0.597 ± 0.236
0.671TrpGlu: 0.671 ± 0.192
0.522TrpPhe: 0.522 ± 0.178
0.746TrpGly: 0.746 ± 0.234
0.298TrpHis: 0.298 ± 0.122
0.895TrpIle: 0.895 ± 0.274
0.522TrpLys: 0.522 ± 0.19
1.119TrpLeu: 1.119 ± 0.354
0.298TrpMet: 0.298 ± 0.12
0.597TrpAsn: 0.597 ± 0.228
0.895TrpPro: 0.895 ± 0.245
0.821TrpGln: 0.821 ± 0.309
0.671TrpArg: 0.671 ± 0.203
1.045TrpSer: 1.045 ± 0.249
0.97TrpThr: 0.97 ± 0.242
0.821TrpVal: 0.821 ± 0.26
0.373TrpTrp: 0.373 ± 0.158
0.597TrpTyr: 0.597 ± 0.197
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.388TyrAla: 2.388 ± 0.478
0.373TyrCys: 0.373 ± 0.214
0.821TyrAsp: 0.821 ± 0.251
1.194TyrGlu: 1.194 ± 0.339
0.97TyrPhe: 0.97 ± 0.255
2.313TyrGly: 2.313 ± 0.348
0.671TyrHis: 0.671 ± 0.226
1.567TyrIle: 1.567 ± 0.275
1.268TyrLys: 1.268 ± 0.264
3.208TyrLeu: 3.208 ± 0.497
0.597TyrMet: 0.597 ± 0.181
1.791TyrAsn: 1.791 ± 0.309
1.567TyrPro: 1.567 ± 0.261
1.567TyrGln: 1.567 ± 0.243
3.059TyrArg: 3.059 ± 0.476
2.313TyrSer: 2.313 ± 0.439
2.611TyrThr: 2.611 ± 0.378
3.134TyrVal: 3.134 ± 0.561
0.522TyrTrp: 0.522 ± 0.182
0.895TyrTyr: 0.895 ± 0.252
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski