Enterobacteria phage SP6 (Bacteriophage SP6)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Zindervirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7Y5R5|Q7Y5R5_BPSP6 Gp4 OS=Enterobacteria phage SP6 OX=194966 GN=4 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 10.67SDD10 pKa = 4.37VYY12 pKa = 9.07NHH14 pKa = 7.44AYY16 pKa = 10.33GLLKK20 pKa = 10.44EE21 pKa = 4.28YY22 pKa = 10.3IRR24 pKa = 11.84YY25 pKa = 9.99DD26 pKa = 3.45DD27 pKa = 4.51VRR29 pKa = 11.84NEE31 pKa = 4.66DD32 pKa = 3.95DD33 pKa = 4.83LSDD36 pKa = 4.87KK37 pKa = 10.64IHH39 pKa = 6.35EE40 pKa = 4.49AAGNAVPHH48 pKa = 6.93WYY50 pKa = 10.57ADD52 pKa = 3.24IFSVMASDD60 pKa = 6.01GIDD63 pKa = 4.05LEE65 pKa = 4.66FDD67 pKa = 4.05DD68 pKa = 5.45SGLMPDD74 pKa = 4.31TKK76 pKa = 10.9DD77 pKa = 3.02VTYY80 pKa = 10.62ILQARR85 pKa = 11.84IHH87 pKa = 5.81EE88 pKa = 4.25QLTIDD93 pKa = 4.64LYY95 pKa = 11.81GDD97 pKa = 3.97AEE99 pKa = 4.37DD100 pKa = 5.41LLNEE104 pKa = 3.94YY105 pKa = 10.52LEE107 pKa = 4.64EE108 pKa = 5.45IEE110 pKa = 5.04AEE112 pKa = 3.93EE113 pKa = 5.4DD114 pKa = 3.71EE115 pKa = 5.13EE116 pKa = 4.46EE117 pKa = 5.13DD118 pKa = 3.74EE119 pKa = 4.62

Molecular weight: 13.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7Y5M7|Q7Y5M7_BPSP6 Gp43 OS=Enterobacteria phage SP6 OX=194966 GN=43 PE=4 SV=1
MM1 pKa = 7.53LRR3 pKa = 11.84HH4 pKa = 6.08QINGNHH10 pKa = 6.02NPLHH14 pKa = 5.08VTGQRR19 pKa = 11.84SRR21 pKa = 11.84SNKK24 pKa = 10.0SIAIQEE30 pKa = 4.12GVPIVRR36 pKa = 11.84ASVLASPTSYY46 pKa = 11.01INDD49 pKa = 3.33PHH51 pKa = 8.51LSGKK55 pKa = 10.04RR56 pKa = 11.84EE57 pKa = 3.71GMMVAVLAPEE67 pKa = 5.32DD68 pKa = 3.87GDD70 pKa = 4.03KK71 pKa = 11.4AGLYY75 pKa = 9.62LYY77 pKa = 10.4RR78 pKa = 11.84WARR81 pKa = 3.33

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13266

25

1270

255.1

28.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.227 ± 0.517

0.95 ± 0.158

6.226 ± 0.192

7.274 ± 0.327

3.611 ± 0.125

8.021 ± 0.407

2.171 ± 0.23

5.676 ± 0.257

6.264 ± 0.308

7.734 ± 0.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.204 ± 0.133

4.018 ± 0.167

3.55 ± 0.198

4.146 ± 0.264

5.571 ± 0.309

5.646 ± 0.269

5.239 ± 0.248

6.747 ± 0.272

1.432 ± 0.123

3.294 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski