Blyttiomyces helicus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Chytridiomycetes; Chytridiomycetes incertae sedis; Blyttiomyces

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P9WG04|A0A4P9WG04_9FUNG Uncharacterized protein OS=Blyttiomyces helicus OX=388810 GN=BDK51DRAFT_52366 PE=4 SV=1
VV1 pKa = 6.27FAPYY5 pKa = 10.81VDD7 pKa = 3.77VTLTPDD13 pKa = 3.21VASLAEE19 pKa = 4.04QTGIMHH25 pKa = 6.37FTLAFLVADD34 pKa = 4.3ASGRR38 pKa = 11.84PNWGEE43 pKa = 3.82TYY45 pKa = 10.71AATDD49 pKa = 3.81PAVVAPIKK57 pKa = 10.43ALRR60 pKa = 11.84ALGGDD65 pKa = 3.75VIVSFGGAAGTEE77 pKa = 4.27LAYY80 pKa = 10.0STANTDD86 pKa = 3.05VKK88 pKa = 10.88QITEE92 pKa = 3.84QYY94 pKa = 10.44LYY96 pKa = 11.03VIDD99 pKa = 4.58ALNVSWVDD107 pKa = 3.86FDD109 pKa = 4.77IEE111 pKa = 4.08GDD113 pKa = 4.04AIADD117 pKa = 3.59IPSVDD122 pKa = 3.42MRR124 pKa = 11.84NAALAAIKK132 pKa = 9.89AAKK135 pKa = 9.12PDD137 pKa = 4.41LIVSYY142 pKa = 7.82TLPVDD147 pKa = 3.43PTGLLDD153 pKa = 3.57EE154 pKa = 4.83GVYY157 pKa = 10.48VLQSASNAGLTVDD170 pKa = 4.06VVNIMTMDD178 pKa = 3.63YY179 pKa = 11.28DD180 pKa = 3.81EE181 pKa = 4.49TVYY184 pKa = 9.86TQGATQMGTYY194 pKa = 10.09AIQATRR200 pKa = 3.27

Molecular weight: 21.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P9WGS3|A0A4P9WGS3_9FUNG Uncharacterized protein OS=Blyttiomyces helicus OX=388810 GN=BDK51DRAFT_43639 PE=4 SV=1
LL1 pKa = 7.62DD2 pKa = 4.48APLPIMTKK10 pKa = 10.32RR11 pKa = 11.84PDD13 pKa = 3.4DD14 pKa = 3.95ATRR17 pKa = 11.84HH18 pKa = 5.52APSKK22 pKa = 11.12ARR24 pKa = 11.84FACNLCMASFTRR36 pKa = 11.84RR37 pKa = 11.84YY38 pKa = 9.72NLHH41 pKa = 5.06QHH43 pKa = 6.2LEE45 pKa = 3.97RR46 pKa = 11.84HH47 pKa = 5.74AGPRR51 pKa = 11.84SKK53 pKa = 10.15QHH55 pKa = 6.62PCDD58 pKa = 3.64LCPRR62 pKa = 11.84AYY64 pKa = 10.09YY65 pKa = 10.35RR66 pKa = 11.84KK67 pKa = 9.91ADD69 pKa = 4.09LARR72 pKa = 11.84HH73 pKa = 5.15MRR75 pKa = 11.84SHH77 pKa = 5.87TRR79 pKa = 3.12

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12137

0

12137

4355336

49

5232

358.8

39.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.921 ± 0.035

1.459 ± 0.01

5.404 ± 0.018

5.84 ± 0.029

3.527 ± 0.017

7.152 ± 0.027

2.391 ± 0.01

4.464 ± 0.018

4.251 ± 0.023

9.023 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.943 ± 0.01

3.151 ± 0.017

7.111 ± 0.036

3.272 ± 0.018

6.81 ± 0.025

8.286 ± 0.03

5.96 ± 0.024

6.578 ± 0.019

1.301 ± 0.008

2.157 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski