Paenibacillus sp. Leaf72

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6399 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4R4F3|A0A0Q4R4F3_9BACL Uncharacterized protein OS=Paenibacillus sp. Leaf72 OX=1736234 GN=ASF12_10020 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 4.95KK3 pKa = 10.45AGYY6 pKa = 10.18ASLWLGKK13 pKa = 10.17LGSEE17 pKa = 4.26EE18 pKa = 4.1QFDD21 pKa = 4.55DD22 pKa = 4.46LLEE25 pKa = 4.53INYY28 pKa = 8.96TEE30 pKa = 5.36DD31 pKa = 3.54GDD33 pKa = 4.84SIPSAFAGHH42 pKa = 6.77FGIEE46 pKa = 4.0WYY48 pKa = 10.24DD49 pKa = 3.46DD50 pKa = 3.66DD51 pKa = 4.71FRR53 pKa = 11.84EE54 pKa = 4.45AEE56 pKa = 4.22FFAEE60 pKa = 4.24EE61 pKa = 4.7DD62 pKa = 3.61RR63 pKa = 11.84GGDD66 pKa = 3.66AANLLAGFSYY76 pKa = 10.89EE77 pKa = 4.27EE78 pKa = 3.66EE79 pKa = 5.07LIPKK83 pKa = 9.35FAALLKK89 pKa = 10.98GMTLDD94 pKa = 3.66EE95 pKa = 5.03FNTVIILYY103 pKa = 8.54NFEE106 pKa = 4.29YY107 pKa = 10.74GGEE110 pKa = 4.02QGSYY114 pKa = 10.73QDD116 pKa = 3.73TSTQLEE122 pKa = 4.83FIGSVPYY129 pKa = 10.27QAA131 pKa = 5.38

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4QB53|A0A0Q4QB53_9BACL Alpha-amylase OS=Paenibacillus sp. Leaf72 OX=1736234 GN=ASF12_21775 PE=4 SV=1
MM1 pKa = 7.61HH2 pKa = 7.27MPNIEE7 pKa = 3.98SSHH10 pKa = 6.88RR11 pKa = 11.84SEE13 pKa = 4.58GNRR16 pKa = 11.84TGGNRR21 pKa = 11.84TGGNRR26 pKa = 11.84TGGNRR31 pKa = 11.84TGGNRR36 pKa = 11.84TGGNRR41 pKa = 11.84TGGNRR46 pKa = 11.84TGGNRR51 pKa = 11.84TGGNRR56 pKa = 11.84TGGNRR61 pKa = 11.84TGGNRR66 pKa = 11.84TGGNRR71 pKa = 11.84TGGNRR76 pKa = 11.84TGGNRR81 pKa = 11.84TGGNRR86 pKa = 11.84TGGNRR91 pKa = 3.25

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6399

0

6399

2116404

26

4618

330.7

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.811 ± 0.037

0.739 ± 0.01

5.0 ± 0.021

6.767 ± 0.033

4.166 ± 0.022

7.317 ± 0.03

2.072 ± 0.014

6.655 ± 0.03

5.323 ± 0.032

10.232 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.016

3.844 ± 0.023

3.904 ± 0.019

4.115 ± 0.02

4.733 ± 0.025

6.502 ± 0.026

5.369 ± 0.03

6.787 ± 0.026

1.286 ± 0.012

3.565 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski