Avon-Heathcote Estuary associated circular virus 5

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IM93|A0A0C5IM93_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 5 OX=1618256 PE=3 SV=1
MM1 pKa = 7.85AANQKK6 pKa = 9.52FKK8 pKa = 11.13NVCFTLNNPGEE19 pKa = 4.28DD20 pKa = 4.27PIPFDD25 pKa = 3.81VEE27 pKa = 3.76KK28 pKa = 10.0MHH30 pKa = 6.15YY31 pKa = 9.75LVYY34 pKa = 10.33QRR36 pKa = 11.84EE37 pKa = 4.2VGANGTEE44 pKa = 4.49HH45 pKa = 5.71YY46 pKa = 10.19QGYY49 pKa = 9.87CEE51 pKa = 5.9LINQTRR57 pKa = 11.84FNAVKK62 pKa = 10.14ALLGGGSVHH71 pKa = 6.44IEE73 pKa = 3.77RR74 pKa = 11.84RR75 pKa = 11.84KK76 pKa = 10.69GSAQEE81 pKa = 3.41AAAYY85 pKa = 8.51CKK87 pKa = 10.34KK88 pKa = 10.66DD89 pKa = 3.45DD90 pKa = 3.77TRR92 pKa = 11.84APGASIQEE100 pKa = 4.29FGEE103 pKa = 4.09MHH105 pKa = 6.99ACTPGKK111 pKa = 10.64RR112 pKa = 11.84NDD114 pKa = 3.42LKK116 pKa = 11.47AFVDD120 pKa = 4.34DD121 pKa = 3.97VRR123 pKa = 11.84SGTKK127 pKa = 9.72RR128 pKa = 11.84KK129 pKa = 9.36RR130 pKa = 11.84DD131 pKa = 3.62LVEE134 pKa = 3.97DD135 pKa = 3.74HH136 pKa = 7.1AGVLARR142 pKa = 11.84YY143 pKa = 7.77PKK145 pKa = 10.29FYY147 pKa = 9.18EE148 pKa = 4.14TLTLMARR155 pKa = 11.84PVRR158 pKa = 11.84TEE160 pKa = 3.56EE161 pKa = 4.0LQVILHH167 pKa = 6.82IGPTGLGKK175 pKa = 8.64TRR177 pKa = 11.84TVMDD181 pKa = 5.21LYY183 pKa = 11.24AADD186 pKa = 4.38PGLYY190 pKa = 8.3VTPLSNGTPWYY201 pKa = 10.04DD202 pKa = 3.56HH203 pKa = 7.01YY204 pKa = 11.43DD205 pKa = 3.3GHH207 pKa = 6.29HH208 pKa = 6.2TVLLDD213 pKa = 3.85DD214 pKa = 4.31FSGRR218 pKa = 11.84SSHH221 pKa = 6.05MSLVTLLRR229 pKa = 11.84LLDD232 pKa = 4.6RR233 pKa = 11.84YY234 pKa = 10.26PVMVPTKK241 pKa = 10.28GAHH244 pKa = 5.33TWWCPNTIYY253 pKa = 9.95VTTNILPSLWYY264 pKa = 8.8EE265 pKa = 3.44WGTRR269 pKa = 11.84GEE271 pKa = 4.37HH272 pKa = 5.23YY273 pKa = 9.9RR274 pKa = 11.84ALARR278 pKa = 11.84RR279 pKa = 11.84FTKK282 pKa = 10.43VVLFYY287 pKa = 11.05EE288 pKa = 4.76KK289 pKa = 10.71LHH291 pKa = 7.08ADD293 pKa = 3.58DD294 pKa = 5.5PGMVEE299 pKa = 5.86QDD301 pKa = 3.56ANWWKK306 pKa = 10.42EE307 pKa = 3.73NCPPEE312 pKa = 4.69AALLYY317 pKa = 10.38SQQ319 pKa = 5.02

Molecular weight: 36.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IM93|A0A0C5IM93_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 5 OX=1618256 PE=3 SV=1
MM1 pKa = 7.36SASGYY6 pKa = 10.15YY7 pKa = 10.04SGNKK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 10.12RR14 pKa = 11.84SDD16 pKa = 3.59FSADD20 pKa = 3.18AQALAVARR28 pKa = 11.84PSFKK32 pKa = 10.16GYY34 pKa = 9.64KK35 pKa = 9.14RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.96EE40 pKa = 3.63FVPGQDD46 pKa = 2.63RR47 pKa = 11.84TGGYY51 pKa = 10.16YY52 pKa = 10.22RR53 pKa = 11.84FGKK56 pKa = 7.96TAMGPGMGEE65 pKa = 3.8MKK67 pKa = 10.66FFDD70 pKa = 3.74TDD72 pKa = 3.17ISEE75 pKa = 4.2LTILDD80 pKa = 3.57GGIVKK85 pKa = 10.66ASMNNVPQGTGEE97 pKa = 3.98SQRR100 pKa = 11.84IGRR103 pKa = 11.84KK104 pKa = 9.13CVVKK108 pKa = 10.49SIYY111 pKa = 10.74LKK113 pKa = 10.79FSVNLPQLEE122 pKa = 4.41NEE124 pKa = 4.52VNMKK128 pKa = 10.56NPDD131 pKa = 3.3QVRR134 pKa = 11.84VILYY138 pKa = 10.1LDD140 pKa = 3.47KK141 pKa = 10.89QANGEE146 pKa = 4.32TTTLGSILEE155 pKa = 4.36GDD157 pKa = 3.88PKK159 pKa = 10.6ISSFRR164 pKa = 11.84NLSNMNRR171 pKa = 11.84YY172 pKa = 7.5TILMDD177 pKa = 3.55KK178 pKa = 10.4VYY180 pKa = 11.1DD181 pKa = 3.65MNYY184 pKa = 9.7EE185 pKa = 4.15SVAAEE190 pKa = 3.92TDD192 pKa = 3.36QGDD195 pKa = 3.33VRR197 pKa = 11.84YY198 pKa = 11.0SMVGKK203 pKa = 7.7TCYY206 pKa = 9.41VSEE209 pKa = 4.01YY210 pKa = 9.98RR211 pKa = 11.84KK212 pKa = 10.53LNIPIEE218 pKa = 4.39FSGTMGAIDD227 pKa = 4.73EE228 pKa = 4.77IGSNNIGLGYY238 pKa = 10.16ISANNVAQIVGQTRR252 pKa = 11.84IRR254 pKa = 11.84YY255 pKa = 9.39ADD257 pKa = 3.13AA258 pKa = 5.34

Molecular weight: 28.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

577

258

319

288.5

32.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.106 ± 0.546

1.386 ± 0.369

5.893 ± 0.047

5.546 ± 0.306

3.293 ± 0.118

8.666 ± 0.853

2.253 ± 1.361

4.333 ± 1.363

5.893 ± 0.421

7.626 ± 1.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.293 ± 0.586

5.199 ± 0.839

4.506 ± 0.849

3.293 ± 0.352

6.412 ± 0.107

5.546 ± 1.566

6.239 ± 0.725

6.759 ± 0.103

1.213 ± 0.733

5.546 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski