Tioman virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Pararubulavirus; Tioman pararubulavirus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8JVB0|Q8JVB0_9MONO Isoform of Q8JVB1 W protein OS=Tioman virus OX=162013 GN=P PE=4 SV=1
MM1 pKa = 7.4SSVFRR6 pKa = 11.84AFEE9 pKa = 4.3LFTLEE14 pKa = 5.1QEE16 pKa = 4.36QQEE19 pKa = 4.48LGNDD23 pKa = 3.2IEE25 pKa = 4.96IPPEE29 pKa = 3.91TLRR32 pKa = 11.84SNIKK36 pKa = 9.81VCILNSQDD44 pKa = 3.01PQTRR48 pKa = 11.84HH49 pKa = 7.0DD50 pKa = 4.01MMCFCLRR57 pKa = 11.84LIASNSARR65 pKa = 11.84AAHH68 pKa = 5.96KK69 pKa = 9.34TGAILTLLNLPTAMMQNHH87 pKa = 5.88IRR89 pKa = 11.84IADD92 pKa = 4.24RR93 pKa = 11.84SPDD96 pKa = 3.38ADD98 pKa = 3.22IEE100 pKa = 4.36RR101 pKa = 11.84IEE103 pKa = 4.01VDD105 pKa = 3.08GFEE108 pKa = 4.55PGTYY112 pKa = 9.65RR113 pKa = 11.84LRR115 pKa = 11.84PNARR119 pKa = 11.84TPLTNGEE126 pKa = 3.93ITALSLMANDD136 pKa = 5.85LPDD139 pKa = 3.74TYY141 pKa = 11.46TNDD144 pKa = 3.58TPFTNHH150 pKa = 6.12RR151 pKa = 11.84AEE153 pKa = 5.09GEE155 pKa = 4.19NCDD158 pKa = 3.69EE159 pKa = 4.45TEE161 pKa = 3.87QFLNAIYY168 pKa = 10.52SVLVQLWVTVCKK180 pKa = 10.64CMTAHH185 pKa = 6.77DD186 pKa = 4.33QPTGSDD192 pKa = 3.06EE193 pKa = 4.13RR194 pKa = 11.84RR195 pKa = 11.84LAKK198 pKa = 10.17YY199 pKa = 8.02QQQGRR204 pKa = 11.84LDD206 pKa = 3.35QKK208 pKa = 11.1YY209 pKa = 10.28ALQPEE214 pKa = 4.95LRR216 pKa = 11.84RR217 pKa = 11.84QIQACIRR224 pKa = 11.84GSLTIRR230 pKa = 11.84QFLTYY235 pKa = 10.1EE236 pKa = 3.87LQTARR241 pKa = 11.84KK242 pKa = 8.48QGAITGRR249 pKa = 11.84YY250 pKa = 7.34YY251 pKa = 11.77AMVGDD256 pKa = 3.52IGKK259 pKa = 10.43YY260 pKa = 9.05IDD262 pKa = 3.75NAGMSAFFMTMRR274 pKa = 11.84FALGTRR280 pKa = 11.84WPPLALSAFSGEE292 pKa = 4.03LLKK295 pKa = 11.09LKK297 pKa = 10.68SLMQLYY303 pKa = 10.58RR304 pKa = 11.84NLGEE308 pKa = 4.16KK309 pKa = 9.58ARR311 pKa = 11.84YY312 pKa = 7.03MALLEE317 pKa = 4.25MPEE320 pKa = 4.02MMEE323 pKa = 4.34FAPANYY329 pKa = 8.35PLCYY333 pKa = 9.75SYY335 pKa = 11.89AMGIGSVQDD344 pKa = 3.3PMMRR348 pKa = 11.84NYY350 pKa = 9.81TFARR354 pKa = 11.84PFLNPAYY361 pKa = 9.55FQLGVEE367 pKa = 4.29TANRR371 pKa = 11.84QQGSVDD377 pKa = 2.85KK378 pKa = 11.56SMAEE382 pKa = 3.8EE383 pKa = 4.3LGLTEE388 pKa = 4.13EE389 pKa = 4.56EE390 pKa = 4.2RR391 pKa = 11.84RR392 pKa = 11.84DD393 pKa = 3.35MSATVTRR400 pKa = 11.84LTTGRR405 pKa = 11.84GAGQAQDD412 pKa = 4.28MINIMGARR420 pKa = 11.84QAAGGRR426 pKa = 11.84GAQGRR431 pKa = 11.84ALRR434 pKa = 11.84IVEE437 pKa = 4.19EE438 pKa = 5.16DD439 pKa = 3.21EE440 pKa = 4.36TTDD443 pKa = 4.67DD444 pKa = 5.07EE445 pKa = 5.44SVDD448 pKa = 3.87DD449 pKa = 4.64QADD452 pKa = 4.39DD453 pKa = 3.44IQGRR457 pKa = 11.84PLPPVPAPIRR467 pKa = 11.84EE468 pKa = 4.21IDD470 pKa = 3.14WEE472 pKa = 3.98ARR474 pKa = 11.84IAEE477 pKa = 4.32IEE479 pKa = 4.17EE480 pKa = 3.71QGQRR484 pKa = 11.84MRR486 pKa = 11.84DD487 pKa = 3.33QGVGRR492 pKa = 11.84LVDD495 pKa = 3.54NQAPNVQNTRR505 pKa = 11.84QNTQDD510 pKa = 3.33NQALLDD516 pKa = 4.0LDD518 pKa = 3.67II519 pKa = 6.3

Molecular weight: 58.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8JVB1|Q8JVB1_9MONO Phosphoprotein OS=Tioman virus OX=162013 GN=P PE=3 SV=1
MM1 pKa = 8.01ALRR4 pKa = 11.84QATIPINVDD13 pKa = 3.2TEE15 pKa = 4.48SEE17 pKa = 4.29KK18 pKa = 11.46NNLNPFPIVPITKK31 pKa = 9.82EE32 pKa = 4.01DD33 pKa = 3.52GSPTGRR39 pKa = 11.84LVKK42 pKa = 9.79QLRR45 pKa = 11.84IKK47 pKa = 10.84NLTPRR52 pKa = 11.84GSTEE56 pKa = 4.25LPLTFINTYY65 pKa = 10.73GFIKK69 pKa = 10.48PLMTYY74 pKa = 8.72TEE76 pKa = 4.46FFSTHH81 pKa = 6.0LRR83 pKa = 11.84TPSCLTACMIPFGAGPFIEE102 pKa = 4.56NPHH105 pKa = 6.88RR106 pKa = 11.84ILDD109 pKa = 3.54KK110 pKa = 10.56CDD112 pKa = 3.0RR113 pKa = 11.84VNIVVRR119 pKa = 11.84KK120 pKa = 7.83SASVKK125 pKa = 10.25EE126 pKa = 4.11EE127 pKa = 3.75IIFDD131 pKa = 3.88VRR133 pKa = 11.84RR134 pKa = 11.84LPPLFNRR141 pKa = 11.84HH142 pKa = 5.38QISGNRR148 pKa = 11.84LICVPSEE155 pKa = 4.41KK156 pKa = 10.2YY157 pKa = 10.28VKK159 pKa = 10.73SPGKK163 pKa = 9.74MIAGTDD169 pKa = 3.53YY170 pKa = 10.53AYY172 pKa = 10.85QIAFVSLTFCPEE184 pKa = 3.79SQKK187 pKa = 10.37FRR189 pKa = 11.84VARR192 pKa = 11.84PLQTIRR198 pKa = 11.84SPIMRR203 pKa = 11.84SVQLEE208 pKa = 4.42VILKK212 pKa = 9.46IDD214 pKa = 3.71CAANSPLKK222 pKa = 10.32RR223 pKa = 11.84FLIISPNSKK232 pKa = 10.66DD233 pKa = 3.23CFASVWFHH241 pKa = 6.1ICNLYY246 pKa = 10.45RR247 pKa = 11.84GNKK250 pKa = 8.09PFKK253 pKa = 10.56AYY255 pKa = 10.46DD256 pKa = 3.37DD257 pKa = 4.34TYY259 pKa = 11.28FSQKK263 pKa = 8.99CRR265 pKa = 11.84AMQLEE270 pKa = 4.42CGIVDD275 pKa = 3.09MWGPTLVVKK284 pKa = 10.71AHH286 pKa = 5.64GKK288 pKa = 7.29IPKK291 pKa = 8.05MARR294 pKa = 11.84PFFSSKK300 pKa = 9.41GWSCHH305 pKa = 5.21AFADD309 pKa = 4.83AAPTLAKK316 pKa = 10.59ALWSVGAQITQVNAILQPSDD336 pKa = 3.11LHH338 pKa = 7.41QLVQISDD345 pKa = 4.21VIWPKK350 pKa = 10.84VKK352 pKa = 10.27LDD354 pKa = 3.94EE355 pKa = 5.62KK356 pKa = 9.24IQSYY360 pKa = 10.08AAAKK364 pKa = 8.17WNPFKK369 pKa = 11.08KK370 pKa = 9.33STNN373 pKa = 3.45

Molecular weight: 42.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

2

8

5118

211

2271

639.8

71.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.585 ± 0.829

2.188 ± 0.252

5.1 ± 0.589

4.982 ± 0.584

3.243 ± 0.389

5.275 ± 0.566

1.837 ± 0.221

7.64 ± 0.554

4.787 ± 0.565

10.746 ± 1.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.403 ± 0.349

5.178 ± 0.443

5.569 ± 0.753

4.572 ± 0.522

5.178 ± 0.509

8.304 ± 0.618

6.643 ± 0.241

5.686 ± 0.484

1.055 ± 0.152

3.029 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski