Changjiang tombus-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFW2|A0A1L3KFW2_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 4 OX=1922818 PE=4 SV=1
MM1 pKa = 7.07IQVPSRR7 pKa = 11.84QGLQAIGGGSTEE19 pKa = 3.83QLRR22 pKa = 11.84SHH24 pKa = 7.04RR25 pKa = 11.84PTLLGLFEE33 pKa = 5.74DD34 pKa = 4.33GWMGYY39 pKa = 9.49PSALSPSTAGDD50 pKa = 3.57VEE52 pKa = 4.66VPKK55 pKa = 10.83AQAEE59 pKa = 4.6VPSNNARR66 pKa = 11.84GPLFGLGEE74 pKa = 4.43SPTAKK79 pKa = 10.0TPGEE83 pKa = 4.35TPFSQVGGTSAPIAPSSGSPRR104 pKa = 11.84SIWAKK109 pKa = 8.88LWSWRR114 pKa = 11.84LGYY117 pKa = 7.62PTTTLSAEE125 pKa = 4.22MADD128 pKa = 3.63ALAVGATTEE137 pKa = 4.11VEE139 pKa = 4.38EE140 pKa = 4.28EE141 pKa = 3.83ALAALDD147 pKa = 3.9EE148 pKa = 4.45QCNVHH153 pKa = 6.66YY154 pKa = 10.8GDD156 pKa = 4.22GQPPSFLGGDD166 pKa = 3.54HH167 pKa = 6.64NFTRR171 pKa = 11.84FAYY174 pKa = 10.17RR175 pKa = 11.84LSIKK179 pKa = 10.61AKK181 pKa = 10.5LKK183 pKa = 10.43FGYY186 pKa = 9.58DD187 pKa = 3.84LKK189 pKa = 10.43PTVANRR195 pKa = 11.84QVVHH199 pKa = 6.11EE200 pKa = 4.1WLVRR204 pKa = 11.84HH205 pKa = 5.45MRR207 pKa = 11.84DD208 pKa = 2.99MNVRR212 pKa = 11.84TTHH215 pKa = 6.68IARR218 pKa = 11.84VLPIAIPLVFVKK230 pKa = 10.55DD231 pKa = 3.93KK232 pKa = 11.05YY233 pKa = 11.21QLDD236 pKa = 3.91ADD238 pKa = 4.15NALAGAPMRR247 pKa = 11.84RR248 pKa = 11.84AAQEE252 pKa = 3.6SRR254 pKa = 11.84KK255 pKa = 7.84TVTEE259 pKa = 3.98RR260 pKa = 11.84WRR262 pKa = 11.84EE263 pKa = 3.75QRR265 pKa = 11.84TWWEE269 pKa = 4.22WLWGTSPRR277 pKa = 11.84PLTFTEE283 pKa = 4.22

Molecular weight: 31.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFV6|A0A1L3KFV6_9VIRU Tombus_P33 domain-containing protein OS=Changjiang tombus-like virus 4 OX=1922818 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.34GNRR6 pKa = 11.84SRR8 pKa = 11.84SKK10 pKa = 10.8RR11 pKa = 11.84KK12 pKa = 9.81GKK14 pKa = 10.4APNAGNTTRR23 pKa = 11.84AKK25 pKa = 10.2PALNLVRR32 pKa = 11.84RR33 pKa = 11.84SRR35 pKa = 11.84LLSLTPAEE43 pKa = 4.03RR44 pKa = 11.84KK45 pKa = 9.14YY46 pKa = 11.27AHH48 pKa = 6.9LVADD52 pKa = 4.42PCNGPLSAGIFGDD65 pKa = 3.78GSGGVISRR73 pKa = 11.84FEE75 pKa = 3.94TDD77 pKa = 4.21GIMGDD82 pKa = 3.79VAPTTASALVFVPAAAAGWTSGKK105 pKa = 9.74PSDD108 pKa = 4.45GDD110 pKa = 3.63APVWNGVTSSLIPGRR125 pKa = 11.84DD126 pKa = 3.61FLNANAGQFRR136 pKa = 11.84CLAACLRR143 pKa = 11.84IYY145 pKa = 10.22WPGTEE150 pKa = 4.88LNRR153 pKa = 11.84QGIVSNLQTTADD165 pKa = 3.14IVNNSNASVGAIRR178 pKa = 11.84ASSTYY183 pKa = 7.19VQRR186 pKa = 11.84MPEE189 pKa = 4.59DD190 pKa = 3.5YY191 pKa = 10.82TEE193 pKa = 4.95LKK195 pKa = 9.18WLPSEE200 pKa = 4.76YY201 pKa = 9.16EE202 pKa = 3.99LEE204 pKa = 3.98MRR206 pKa = 11.84APGVIAASQEE216 pKa = 3.84FSRR219 pKa = 11.84FSALVTTTSGVPSATPIRR237 pKa = 11.84WRR239 pKa = 11.84MVAVYY244 pKa = 8.94EE245 pKa = 3.98WVPRR249 pKa = 11.84VSTGLSSMNTAASVPAGSFQRR270 pKa = 11.84VTQALTSIGNWAYY283 pKa = 10.94VSSHH287 pKa = 5.08QAATAISSLLAGAHH301 pKa = 6.2AAGTLASGVATLTLGG316 pKa = 3.54

Molecular weight: 33.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1075

283

476

358.3

39.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.07 ± 1.262

1.209 ± 0.368

3.535 ± 0.27

4.744 ± 0.702

3.535 ± 0.49

8.837 ± 0.153

2.233 ± 0.562

3.814 ± 0.362

3.349 ± 0.327

8.0 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.341

3.721 ± 0.494

5.767 ± 0.718

3.535 ± 0.404

8.0 ± 0.772

7.535 ± 1.313

6.233 ± 1.062

7.163 ± 0.404

2.512 ± 0.343

2.698 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski