Streptococcus phage Javan370

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B080|A0A4D6B080_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan370 OX=2548131 GN=Javan370_0004 PE=4 SV=1
MM1 pKa = 7.67IIKK4 pKa = 9.94DD5 pKa = 3.75YY6 pKa = 10.68KK7 pKa = 10.79YY8 pKa = 10.81EE9 pKa = 4.0NSTDD13 pKa = 4.09GIHH16 pKa = 6.89YY17 pKa = 10.31IIDD20 pKa = 3.16VDD22 pKa = 4.23GYY24 pKa = 9.41EE25 pKa = 4.26FEE27 pKa = 4.59MNHH30 pKa = 5.37TRR32 pKa = 11.84TDD34 pKa = 3.68YY35 pKa = 11.55GSVQHH40 pKa = 7.37DD41 pKa = 4.27DD42 pKa = 3.23IEE44 pKa = 4.6YY45 pKa = 9.64FLNEE49 pKa = 3.33IAEE52 pKa = 4.18YY53 pKa = 11.14DD54 pKa = 3.6MQEE57 pKa = 3.96AEE59 pKa = 4.64LIEE62 pKa = 4.63DD63 pKa = 4.27FVRR66 pKa = 11.84FQSYY70 pKa = 10.66LLMYY74 pKa = 10.69GVGFTFKK81 pKa = 10.68NFEE84 pKa = 4.11EE85 pKa = 4.38VQDD88 pKa = 3.95DD89 pKa = 4.32SKK91 pKa = 11.98VV92 pKa = 3.16

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B1W1|A0A4D6B1W1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan370 OX=2548131 GN=Javan370_0038 PE=4 SV=1
MM1 pKa = 7.45NIVDD5 pKa = 5.42PIRR8 pKa = 11.84DD9 pKa = 3.51KK10 pKa = 11.75DD11 pKa = 4.23DD12 pKa = 3.25IQAMKK17 pKa = 10.42EE18 pKa = 3.85YY19 pKa = 10.29LRR21 pKa = 11.84EE22 pKa = 4.1WNEE25 pKa = 3.51RR26 pKa = 11.84NYY28 pKa = 11.33LLFLFGINSGLRR40 pKa = 11.84VGDD43 pKa = 3.52ILRR46 pKa = 11.84IRR48 pKa = 11.84VKK50 pKa = 10.46DD51 pKa = 3.58VQGWYY56 pKa = 10.02IKK58 pKa = 10.34IKK60 pKa = 7.62EE61 pKa = 4.14QKK63 pKa = 7.74TGKK66 pKa = 10.0RR67 pKa = 11.84KK68 pKa = 7.68QLKK71 pKa = 5.95MTKK74 pKa = 7.3TLKK77 pKa = 10.69KK78 pKa = 9.92EE79 pKa = 3.49VRR81 pKa = 11.84EE82 pKa = 4.22YY83 pKa = 10.69IKK85 pKa = 10.86DD86 pKa = 3.73MPLHH90 pKa = 6.72HH91 pKa = 6.98YY92 pKa = 9.94LFQSRR97 pKa = 11.84IGKK100 pKa = 8.82NKK102 pKa = 9.05PLDD105 pKa = 3.58RR106 pKa = 11.84RR107 pKa = 11.84TVDD110 pKa = 3.65WILKK114 pKa = 7.15TAAIEE119 pKa = 4.16CGIEE123 pKa = 4.01NIGTHH128 pKa = 5.65SMRR131 pKa = 11.84KK132 pKa = 6.69TFGYY136 pKa = 10.09HH137 pKa = 5.2YY138 pKa = 10.17YY139 pKa = 11.04KK140 pKa = 9.51KK141 pKa = 8.83TKK143 pKa = 10.1DD144 pKa = 3.27VAMLMDD150 pKa = 5.13LFNHH154 pKa = 6.42SSPAITLRR162 pKa = 11.84YY163 pKa = 9.27IGIRR167 pKa = 11.84QDD169 pKa = 3.47QRR171 pKa = 11.84DD172 pKa = 3.53KK173 pKa = 11.9AMSNFDD179 pKa = 3.56LL180 pKa = 5.05

Molecular weight: 21.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11397

41

1427

207.2

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.984 ± 0.713

0.439 ± 0.095

6.66 ± 0.337

8.178 ± 0.377

4.422 ± 0.3

6.344 ± 0.422

1.307 ± 0.132

6.923 ± 0.337

8.529 ± 0.332

8.529 ± 0.361

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.132

5.931 ± 0.236

2.659 ± 0.18

4.273 ± 0.321

3.966 ± 0.261

6.098 ± 0.264

6.01 ± 0.275

5.931 ± 0.258

1.123 ± 0.098

3.238 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski