Mycobacterium phage ShedlockHolmes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6YR54|A0A0F6YR54_9CAUD Uncharacterized protein OS=Mycobacterium phage ShedlockHolmes OX=1647313 GN=62 PE=4 SV=1
MM1 pKa = 7.3YY2 pKa = 10.47SAFDD6 pKa = 4.26DD7 pKa = 4.03AWSGTATHH15 pKa = 6.41SVLAAIAGTFGVPIGDD31 pKa = 3.59LAEE34 pKa = 4.34PVAVAA39 pKa = 4.72

Molecular weight: 3.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6YR35|A0A0F6YR35_9CAUD Uncharacterized protein OS=Mycobacterium phage ShedlockHolmes OX=1647313 GN=87 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84RR3 pKa = 11.84PSQLATGTDD12 pKa = 3.81LCQKK16 pKa = 9.94RR17 pKa = 11.84AKK19 pKa = 9.93CLQIEE24 pKa = 4.48QIGTDD29 pKa = 3.35RR30 pKa = 11.84EE31 pKa = 4.54TQSVLAFPQVKK42 pKa = 10.19DD43 pKa = 3.39SFKK46 pKa = 10.24SVVYY50 pKa = 10.51RR51 pKa = 11.84STDD54 pKa = 3.26VFKK57 pKa = 10.48LTTLIQDD64 pKa = 3.38SRR66 pKa = 11.84RR67 pKa = 11.84FAWSLSGRR75 pKa = 11.84GRR77 pKa = 11.84VCRR80 pKa = 11.84SYY82 pKa = 11.91GEE84 pKa = 3.91ICRR87 pKa = 11.84SVDD90 pKa = 3.19TPRR93 pKa = 11.84CSKK96 pKa = 10.45HH97 pKa = 6.16HH98 pKa = 6.26GPSGGRR104 pKa = 11.84AVRR107 pKa = 11.84SGRR110 pKa = 3.48

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

19023

32

1382

190.2

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.358 ± 0.475

1.172 ± 0.165

6.487 ± 0.219

5.767 ± 0.397

2.749 ± 0.135

9.01 ± 0.546

2.013 ± 0.161

3.732 ± 0.183

3.433 ± 0.206

8.138 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.055 ± 0.09

2.576 ± 0.23

5.698 ± 0.272

3.648 ± 0.207

6.797 ± 0.418

5.094 ± 0.259

5.972 ± 0.257

7.964 ± 0.229

2.092 ± 0.105

2.245 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski