Elsinoe australis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Myriangiales; Elsinoaceae; Elsinoe

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P7YG43|A0A2P7YG43_9PEZI Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Elsinoe australis OX=40998 GN=B9Z65_1521 PE=4 SV=1
MM1 pKa = 7.47EE2 pKa = 5.96PSTGVSASCAYY13 pKa = 7.5LTPSCGHH20 pKa = 5.89QFLEE24 pKa = 4.34AKK26 pKa = 9.14SWNRR30 pKa = 11.84GWTGDD35 pKa = 3.12GVTILEE41 pKa = 4.63CGRR44 pKa = 11.84KK45 pKa = 9.39DD46 pKa = 3.28RR47 pKa = 11.84LRR49 pKa = 11.84WNCEE53 pKa = 3.4EE54 pKa = 4.1GLCLHH59 pKa = 6.57MPMDD63 pKa = 5.37EE64 pKa = 6.2DD65 pKa = 4.43GCCYY69 pKa = 10.57NGWSLEE75 pKa = 3.89SDD77 pKa = 3.35QEE79 pKa = 4.32GNEE82 pKa = 4.24EE83 pKa = 4.19KK84 pKa = 10.88NEE86 pKa = 3.94EE87 pKa = 4.21DD88 pKa = 5.51DD89 pKa = 5.16EE90 pKa = 5.63EE91 pKa = 6.35DD92 pKa = 5.56DD93 pKa = 4.96EE94 pKa = 6.31DD95 pKa = 4.94EE96 pKa = 4.32EE97 pKa = 5.92DD98 pKa = 4.17EE99 pKa = 4.53GSEE102 pKa = 4.47GNEE105 pKa = 4.03DD106 pKa = 4.36DD107 pKa = 4.28EE108 pKa = 5.0

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8A8D5|A0A2P8A8D5_9PEZI SAC3 family protein 1 OS=Elsinoe australis OX=40998 GN=B9Z65_6341 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9222

0

9222

4626512

41

7744

501.7

55.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.879 ± 0.021

1.063 ± 0.009

5.745 ± 0.021

6.207 ± 0.026

3.568 ± 0.017

7.28 ± 0.027

2.243 ± 0.01

4.603 ± 0.018

5.028 ± 0.024

8.492 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.009

3.632 ± 0.014

6.037 ± 0.026

4.142 ± 0.021

6.131 ± 0.025

8.331 ± 0.033

6.239 ± 0.024

6.118 ± 0.02

1.387 ± 0.009

2.689 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski