Beijerinckiaceae bacterium RH AL1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; unclassified Beijerinckiaceae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E4NUA7|A0A5E4NUA7_9RHIZ RNA polymerase-binding transcription factor DksA OS=Beijerinckiaceae bacterium RH AL1 OX=2572036 GN=dksA PE=3 SV=1
MM1 pKa = 7.49SLFNSMRR8 pKa = 11.84TAVSGMAAQSNALSAISDD26 pKa = 4.06NIANSSTVGYY36 pKa = 10.44KK37 pKa = 10.06DD38 pKa = 3.39AQAQFEE44 pKa = 4.58TVLDD48 pKa = 3.82QNATSEE54 pKa = 4.39YY55 pKa = 10.2EE56 pKa = 4.15SGGVQTDD63 pKa = 2.53IRR65 pKa = 11.84YY66 pKa = 8.93GVSTQGTLEE75 pKa = 4.28TTTSSTDD82 pKa = 3.11MAINGNGFFVVSNGGQGTYY101 pKa = 8.06LTRR104 pKa = 11.84AGSFVPDD111 pKa = 3.32STGNLVNTAGYY122 pKa = 9.53QLQGYY127 pKa = 8.87KK128 pKa = 10.03INPDD132 pKa = 3.02GTTSSTLSTVNINNQTLQAAASTTGTLTANLPSTATAVAANTPGTSNSTSTTYY185 pKa = 10.32TDD187 pKa = 3.14KK188 pKa = 11.37TSITVYY194 pKa = 11.02DD195 pKa = 4.22DD196 pKa = 4.79LGTSHH201 pKa = 7.33VMDD204 pKa = 4.14VYY206 pKa = 10.26LTKK209 pKa = 10.41TGNNTWEE216 pKa = 3.91AAAYY220 pKa = 6.94PQADD224 pKa = 3.47ASASGGFPYY233 pKa = 10.6SSGGTTDD240 pKa = 4.77AVATPTTLKK249 pKa = 10.53FDD251 pKa = 3.73PTTGNMLNTPVTMNIQIPGGQAGQTVAVNLSATTQLASSFAVTAGTADD299 pKa = 3.33GNAPSKK305 pKa = 10.71LSNVKK310 pKa = 9.94IGTDD314 pKa = 3.5GTVTEE319 pKa = 5.5VYY321 pKa = 10.82ASGYY325 pKa = 9.59QLAAYY330 pKa = 7.7KK331 pKa = 10.33IPLATVEE338 pKa = 4.58SPTNLTNLSGNVYY351 pKa = 10.0QVSQNSGPMVLSTATTNGTGSILADD376 pKa = 3.51TLEE379 pKa = 4.35EE380 pKa = 4.33STVDD384 pKa = 4.44LATEE388 pKa = 4.53LTNMISAQRR397 pKa = 11.84SYY399 pKa = 10.86EE400 pKa = 4.04ANSKK404 pKa = 9.58VLQAASDD411 pKa = 3.9LLGDD415 pKa = 4.53LNRR418 pKa = 11.84LTTNN422 pKa = 3.32

Molecular weight: 43.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E4NW26|A0A5E4NW26_9RHIZ Dihydrofolate synthase/folylpolyglutamate synthase OS=Beijerinckiaceae bacterium RH AL1 OX=2572036 GN=RHAL1_01252 PE=3 SV=1
MM1 pKa = 7.67LKK3 pKa = 9.02TAIAASIVLGSVSFASAMPMASSVQTGASLPVVKK37 pKa = 10.34AAVIVKK43 pKa = 9.72KK44 pKa = 9.13VVRR47 pKa = 11.84RR48 pKa = 11.84PPVRR52 pKa = 11.84RR53 pKa = 11.84TVVRR57 pKa = 11.84KK58 pKa = 8.19TIIRR62 pKa = 11.84RR63 pKa = 3.52

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4238

0

4238

1273430

29

2807

300.5

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.971 ± 0.061

0.892 ± 0.013

5.82 ± 0.032

5.278 ± 0.035

3.618 ± 0.028

8.487 ± 0.039

2.13 ± 0.017

4.761 ± 0.027

3.237 ± 0.035

10.013 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.017

2.201 ± 0.025

5.663 ± 0.034

2.813 ± 0.022

7.37 ± 0.047

5.093 ± 0.029

5.419 ± 0.038

7.548 ± 0.031

1.24 ± 0.015

2.158 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski