Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium; Penicillium chrysogenum species complex; Penicillium rubens

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12788 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6HIF0|B6HIF0_PENRW Pc21g05930 protein OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=Pc21g05930 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84TQQLLPLCGLLLASVTIASKK22 pKa = 10.73LDD24 pKa = 3.55HH25 pKa = 7.68DD26 pKa = 5.06DD27 pKa = 3.5VPNRR31 pKa = 11.84CWPACGPVVGIANSCDD47 pKa = 3.12ARR49 pKa = 11.84HH50 pKa = 5.93EE51 pKa = 4.22RR52 pKa = 11.84DD53 pKa = 3.38SAEE56 pKa = 4.0IQCICDD62 pKa = 2.94WDD64 pKa = 3.85AAKK67 pKa = 8.95TQIPLCAACITQYY80 pKa = 9.23QTDD83 pKa = 3.5RR84 pKa = 11.84RR85 pKa = 11.84NNNTTRR91 pKa = 11.84HH92 pKa = 5.9DD93 pKa = 3.83HH94 pKa = 7.79DD95 pKa = 6.81DD96 pKa = 5.18DD97 pKa = 6.24DD98 pKa = 5.94DD99 pKa = 6.61HH100 pKa = 9.43DD101 pKa = 5.85DD102 pKa = 5.18DD103 pKa = 6.81HH104 pKa = 9.36DD105 pKa = 5.32DD106 pKa = 5.16DD107 pKa = 6.69HH108 pKa = 9.19DD109 pKa = 5.43DD110 pKa = 3.63DD111 pKa = 5.78HH112 pKa = 9.6DD113 pKa = 4.46YY114 pKa = 11.71DD115 pKa = 4.63RR116 pKa = 11.84DD117 pKa = 3.68NDD119 pKa = 3.67NDD121 pKa = 4.15IDD123 pKa = 5.4DD124 pKa = 5.56DD125 pKa = 4.89DD126 pKa = 6.11NEE128 pKa = 4.39ALDD131 pKa = 3.67LVRR134 pKa = 11.84YY135 pKa = 9.23CKK137 pKa = 9.98LTTTTYY143 pKa = 10.94NSAAATTMGTSTSTAGANTATTTDD167 pKa = 3.76PSNAATGTDD176 pKa = 3.38SAGTGGTNSQDD187 pKa = 3.5STDD190 pKa = 3.7SAGASAGSASSPTPTPDD207 pKa = 2.9AAAGILAPGAASMAGVMGIMALAWLL232 pKa = 4.4

Molecular weight: 24.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6GWN6|B6GWN6_PENRW Uncharacterized protein (Fragment) OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=Pc11g00010 PE=4 SV=1
AA1 pKa = 7.57LRR3 pKa = 11.84PTTVSRR9 pKa = 11.84RR10 pKa = 11.84IGSASTTVSRR20 pKa = 11.84RR21 pKa = 11.84TGSASTTVSRR31 pKa = 11.84RR32 pKa = 11.84IGSAISTVSRR42 pKa = 11.84RR43 pKa = 11.84IGSASTTVSRR53 pKa = 11.84RR54 pKa = 11.84TGSASTTVSRR64 pKa = 11.84RR65 pKa = 11.84IGSASTTVSRR75 pKa = 11.84RR76 pKa = 11.84IGSASTTVSRR86 pKa = 11.84RR87 pKa = 11.84TGSASTTVSRR97 pKa = 11.84RR98 pKa = 11.84TGSAA102 pKa = 2.77

Molecular weight: 10.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12788

0

12788

5708734

11

7287

446.4

49.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.27 ± 0.02

1.349 ± 0.009

5.599 ± 0.013

6.05 ± 0.02

3.763 ± 0.012

6.742 ± 0.021

2.489 ± 0.01

5.069 ± 0.015

4.614 ± 0.019

9.009 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.009

3.697 ± 0.011

6.095 ± 0.024

4.02 ± 0.015

6.173 ± 0.02

8.414 ± 0.022

5.931 ± 0.013

6.136 ± 0.021

1.491 ± 0.009

2.823 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski