Achromobacter phage JWF

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5A273|A0A0B5A273_9CAUD Uncharacterized protein OS=Achromobacter phage JWF OX=1589748 GN=PJWF_00080 PE=4 SV=1
MM1 pKa = 7.82ASTTTIPCAHH11 pKa = 7.01CGAPLILDD19 pKa = 3.46VGEE22 pKa = 4.64FMPEE26 pKa = 3.64GKK28 pKa = 10.25LDD30 pKa = 3.71LTLEE34 pKa = 4.44CSNCEE39 pKa = 3.97APTLNAFVPFADD51 pKa = 5.46FEE53 pKa = 4.33ALEE56 pKa = 4.15

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4ZZW2|A0A0B4ZZW2_9CAUD Uncharacterized protein OS=Achromobacter phage JWF OX=1589748 GN=PJWF_00074 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84SIRR5 pKa = 11.84IAIVGAVMGLAACFAGQAQAIDD27 pKa = 3.54IVQTSTYY34 pKa = 10.72ASLHH38 pKa = 6.41AYY40 pKa = 9.81AEE42 pKa = 4.06PAAAVSSAPASYY54 pKa = 10.79VDD56 pKa = 3.71QVLAQAQAAPAIALLLAGLGLLALFLLASAPMRR89 pKa = 11.84RR90 pKa = 11.84MARR93 pKa = 11.84VATAVMQLLWRR104 pKa = 11.84RR105 pKa = 11.84DD106 pKa = 3.27VRR108 pKa = 11.84PWAKK112 pKa = 10.57VLMRR116 pKa = 11.84DD117 pKa = 4.3KK118 pKa = 11.06RR119 pKa = 11.84PGPLKK124 pKa = 10.34RR125 pKa = 11.84VAWPTNWHH133 pKa = 6.27LADD136 pKa = 4.22PCSPLL141 pKa = 3.63

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

116

0

116

25040

39

1827

215.9

24.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.505 ± 0.528

0.871 ± 0.097

6.146 ± 0.164

6.378 ± 0.242

3.474 ± 0.167

6.957 ± 0.202

2.296 ± 0.197

5.012 ± 0.205

5.164 ± 0.19

8.51 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.127 ± 0.103

4.365 ± 0.156

4.445 ± 0.221

4.297 ± 0.241

5.923 ± 0.169

5.763 ± 0.232

6.318 ± 0.231

6.781 ± 0.189

1.569 ± 0.131

3.099 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski