Thioclava dalianensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thioclava

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074TA72|A0A074TA72_9RHOB Cytochrome c domain-containing protein OS=Thioclava dalianensis OX=1185766 GN=DL1_09710 PE=4 SV=1
MM1 pKa = 7.07TASPDD6 pKa = 3.62EE7 pKa = 4.44PLEE10 pKa = 4.12GAPLIKK16 pKa = 10.06PSSTDD21 pKa = 2.97HH22 pKa = 6.87PLYY25 pKa = 11.02DD26 pKa = 4.07PVVDD30 pKa = 4.07ACRR33 pKa = 11.84TVYY36 pKa = 10.75DD37 pKa = 3.83PEE39 pKa = 5.17IPVNIYY45 pKa = 10.8DD46 pKa = 4.42LGLIYY51 pKa = 10.15TIEE54 pKa = 3.88ITPEE58 pKa = 3.47NDD60 pKa = 2.89AKK62 pKa = 11.32VIMTLTAPGCPVAGEE77 pKa = 4.16MPGWVHH83 pKa = 7.19DD84 pKa = 4.33AVASVGGLRR93 pKa = 11.84DD94 pKa = 3.17VDD96 pKa = 3.83VEE98 pKa = 4.49MTFDD102 pKa = 4.09PQWGMDD108 pKa = 3.61MMSDD112 pKa = 3.68EE113 pKa = 4.89ARR115 pKa = 11.84LEE117 pKa = 4.14LGFMM121 pKa = 4.54

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074TBQ2|A0A074TBQ2_9RHOB Signal peptidase I OS=Thioclava dalianensis OX=1185766 GN=DL1_05505 PE=3 SV=1
MM1 pKa = 7.54TNGPDD6 pKa = 3.25IASIAALIADD16 pKa = 4.55PARR19 pKa = 11.84ALMLNALMAGRR30 pKa = 11.84ALSAGEE36 pKa = 3.8LAGIAGIAPSTASSHH51 pKa = 6.25LARR54 pKa = 11.84LSAGGLVAMRR64 pKa = 11.84QQGRR68 pKa = 11.84HH69 pKa = 5.09RR70 pKa = 11.84YY71 pKa = 9.14FEE73 pKa = 4.2ISGSAAVEE81 pKa = 3.92LLEE84 pKa = 4.07SLMRR88 pKa = 11.84FSTVTLPRR96 pKa = 11.84LGPRR100 pKa = 11.84DD101 pKa = 3.28ADD103 pKa = 3.41LRR105 pKa = 11.84EE106 pKa = 3.93ARR108 pKa = 11.84LCYY111 pKa = 10.33NHH113 pKa = 6.98LAGRR117 pKa = 11.84AGVALYY123 pKa = 10.62DD124 pKa = 3.59GLIARR129 pKa = 11.84GALCPGHH136 pKa = 7.3DD137 pKa = 3.97GPEE140 pKa = 3.93LTEE143 pKa = 3.77TGRR146 pKa = 11.84AFVQTLGIDD155 pKa = 4.04LSAKK159 pKa = 6.85TFKK162 pKa = 10.63RR163 pKa = 11.84PPICRR168 pKa = 11.84SCLDD172 pKa = 2.99WSEE175 pKa = 4.31RR176 pKa = 11.84RR177 pKa = 11.84SHH179 pKa = 7.01LGGALGRR186 pKa = 11.84ALWEE190 pKa = 4.67AIATKK195 pKa = 10.08RR196 pKa = 11.84WARR199 pKa = 11.84QCGDD203 pKa = 3.0SRR205 pKa = 11.84VVRR208 pKa = 11.84FTPEE212 pKa = 3.41GQRR215 pKa = 11.84RR216 pKa = 11.84FAQLLAPKK224 pKa = 10.03TSMQGGKK231 pKa = 9.68SGQKK235 pKa = 9.57PRR237 pKa = 11.84LPAA240 pKa = 4.82

Molecular weight: 25.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4007

0

4007

1230192

39

4774

307.0

33.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.642 ± 0.052

0.869 ± 0.013

5.805 ± 0.033

5.8 ± 0.04

3.642 ± 0.023

8.64 ± 0.042

2.022 ± 0.019

5.241 ± 0.027

3.293 ± 0.035

10.188 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.763 ± 0.019

2.479 ± 0.022

5.23 ± 0.028

3.369 ± 0.024

6.794 ± 0.039

5.385 ± 0.028

5.312 ± 0.03

6.927 ± 0.035

1.418 ± 0.018

2.181 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski