Mitosporidium daphniae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Microsporidia incertae sedis; Mitosporidium

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A098VPZ4|A0A098VPZ4_9MICR Aa_trans domain-containing protein OS=Mitosporidium daphniae OX=1485682 GN=DI09_44p20 PE=3 SV=1
NN1 pKa = 6.07TGYY4 pKa = 11.21YY5 pKa = 10.33LLNASEE11 pKa = 4.12WGVPEE16 pKa = 4.28VSATYY21 pKa = 10.97SLTIDD26 pKa = 3.4SMLGSGSIEE35 pKa = 3.32LTMFDD40 pKa = 4.48KK41 pKa = 10.74VTLNVTIDD49 pKa = 3.58PSIVLSDD56 pKa = 3.97DD57 pKa = 3.43GAVNVNWSPIDD68 pKa = 3.57PSEE71 pKa = 4.19PFVNLSVYY79 pKa = 10.53NSSNSLLKK87 pKa = 10.04TFEE90 pKa = 4.06VANTGSYY97 pKa = 10.98SNDD100 pKa = 3.0VKK102 pKa = 11.34SLGLFPGGYY111 pKa = 9.68KK112 pKa = 9.81IQVDD116 pKa = 4.02SNMGFGFNHH125 pKa = 6.45FLVYY129 pKa = 10.48SATPLNILVPNSEE142 pKa = 4.39SIIYY146 pKa = 10.02NNSASLSVQWTPFDD160 pKa = 4.35EE161 pKa = 4.58NVLPAIITIVNQNTMEE177 pKa = 4.27NNSFAVTNNGNASLDD192 pKa = 3.69LSSIDD197 pKa = 4.22VPAGTYY203 pKa = 9.84NISVEE208 pKa = 4.31TVIGKK213 pKa = 9.63SKK215 pKa = 10.07SPSMFVLLYY224 pKa = 10.48PEE226 pKa = 4.43TLLIASPNSTT236 pKa = 3.47

Molecular weight: 25.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A098VN14|A0A098VN14_9MICR 60S ribosomal protein L30 OS=Mitosporidium daphniae OX=1485682 GN=DI09_76p90 PE=3 SV=1
MM1 pKa = 7.66AVPFAISSEE10 pKa = 4.09RR11 pKa = 11.84QQYY14 pKa = 9.64APLWMQAPAAPSRR27 pKa = 11.84CSVATLRR34 pKa = 11.84FADD37 pKa = 4.1LASKK41 pKa = 10.39RR42 pKa = 11.84VNNKK46 pKa = 8.6AHH48 pKa = 6.54TSRR51 pKa = 11.84IVKK54 pKa = 10.07SRR56 pKa = 11.84FSISDD61 pKa = 3.24TWCATLTARR70 pKa = 11.84WISSAVSCFLKK81 pKa = 10.87

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3256

0

3256

1247860

31

4452

383.2

42.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.162 ± 0.047

1.76 ± 0.022

5.087 ± 0.027

5.951 ± 0.042

4.992 ± 0.037

5.24 ± 0.034

2.367 ± 0.018

6.729 ± 0.035

5.891 ± 0.05

10.581 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.015

4.175 ± 0.031

5.143 ± 0.034

3.525 ± 0.026

4.77 ± 0.039

10.452 ± 0.048

4.885 ± 0.026

5.375 ± 0.032

1.009 ± 0.014

2.804 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski