Muricauda lutaonensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Muricauda

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2882 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0D5YVH3|A0A0D5YVH3_9FLAO Uncharacterized protein OS=Muricauda lutaonensis OX=516051 GN=VC82_2257 PE=4 SV=1
MM1 pKa = 7.49GKK3 pKa = 8.33MLKK6 pKa = 9.95IFVLFFLLTISIANAQDD23 pKa = 3.15TYY25 pKa = 11.34YY26 pKa = 10.24DD27 pKa = 3.72TFSSVSYY34 pKa = 10.67SNNDD38 pKa = 2.73GTQNWSTNWIEE49 pKa = 4.62SGDD52 pKa = 4.03DD53 pKa = 3.46NSPSGGYY60 pKa = 9.44IRR62 pKa = 11.84ITGNQLRR69 pKa = 11.84FAYY72 pKa = 9.21IWTEE76 pKa = 3.95TIRR79 pKa = 11.84RR80 pKa = 11.84SADD83 pKa = 2.89LSAYY87 pKa = 10.5SSATLSFDD95 pKa = 2.93WQTSSLEE102 pKa = 4.13SGEE105 pKa = 4.19TLDD108 pKa = 4.32VQVSSDD114 pKa = 3.04GSSYY118 pKa = 7.99TTLATFSGNQSGTFSQNISAHH139 pKa = 5.8ISSNTTIRR147 pKa = 11.84FIKK150 pKa = 10.4GGNNWSGNNDD160 pKa = 2.74RR161 pKa = 11.84VYY163 pKa = 10.71IDD165 pKa = 3.91NVLITATATASVSIADD181 pKa = 3.55VSVNEE186 pKa = 4.27GDD188 pKa = 3.54GTATFMVQHH197 pKa = 6.47VGPNATGAFTVNFTTNDD214 pKa = 3.2GTAVAGSDD222 pKa = 3.56YY223 pKa = 9.81TASSGTLSFNGTSGDD238 pKa = 3.86TEE240 pKa = 5.27SITVPINDD248 pKa = 4.52DD249 pKa = 3.56GDD251 pKa = 4.15FEE253 pKa = 4.46GLEE256 pKa = 4.24TFTVSFTGTSDD267 pKa = 3.23GTINISDD274 pKa = 4.01TATGSITDD282 pKa = 3.94NDD284 pKa = 4.33LLGNTPLALFEE295 pKa = 5.23AFDD298 pKa = 4.64GYY300 pKa = 11.75VDD302 pKa = 3.73YY303 pKa = 10.63TSTGGTLRR311 pKa = 11.84TQPNTGDD318 pKa = 3.02ACAITTSSSNTLTSSIPATATIQKK342 pKa = 10.6ALLYY346 pKa = 7.77WAHH349 pKa = 6.44SAATPDD355 pKa = 3.46SQVTFEE361 pKa = 4.74GNTVTADD368 pKa = 3.47LMYY371 pKa = 7.82TTTLTNRR378 pKa = 11.84VFFGGVSDD386 pKa = 3.7VTSIVQGISNPSTNTYY402 pKa = 11.07DD403 pKa = 3.75FTDD406 pKa = 3.63LTVDD410 pKa = 3.25NTGNYY415 pKa = 9.69CSTATVLGGWSLFIFYY431 pKa = 10.47EE432 pKa = 4.43DD433 pKa = 3.65ASLPASTVNLYY444 pKa = 10.58QGFNGEE450 pKa = 4.72SNSSSSFSLSGFFAIGASGSKK471 pKa = 7.02TTVLSWEE478 pKa = 4.35GDD480 pKa = 3.31QTLSNNEE487 pKa = 4.26LLTVTTGLGTFTLAGDD503 pKa = 3.95GDD505 pKa = 4.49NNGVAVNNPFNSTIFDD521 pKa = 3.34NTIIPNVNNASAYY534 pKa = 10.45GLDD537 pKa = 3.84LDD539 pKa = 4.69TYY541 pKa = 10.88DD542 pKa = 3.65VSPYY546 pKa = 9.34IQPGEE551 pKa = 4.33STVTTNVQSGQDD563 pKa = 3.7FVIMNALVLKK573 pKa = 10.55VPSNLITGTVFEE585 pKa = 4.77DD586 pKa = 3.34VNYY589 pKa = 11.09GGGAGRR595 pKa = 11.84NRR597 pKa = 11.84ASSSGVGVQGATVEE611 pKa = 5.45LYY613 pKa = 10.24DD614 pKa = 4.31ALNNFVDD621 pKa = 4.06NTTTDD626 pKa = 2.97ANGLYY631 pKa = 10.26RR632 pKa = 11.84FGGMANGSYY641 pKa = 9.93SVRR644 pKa = 11.84VVNGTVRR651 pKa = 11.84STRR654 pKa = 11.84GGGAACNTCWPVQTFRR670 pKa = 11.84TSFPGGSVIEE680 pKa = 4.33VTNEE684 pKa = 3.38VGGADD689 pKa = 3.81PSAEE693 pKa = 3.89DD694 pKa = 3.55SAAGTLTGAQSVAPVLITSEE714 pKa = 4.62GVVGMDD720 pKa = 3.81FGYY723 pKa = 11.06NFNTIVNTNEE733 pKa = 4.11DD734 pKa = 3.71GQGSLEE740 pKa = 3.96QFIVNANNLDD750 pKa = 4.17EE751 pKa = 5.45IGLDD755 pKa = 3.1IVANSIFDD763 pKa = 3.84PAAGQDD769 pKa = 3.34TSIFMIPSSSDD780 pKa = 3.21PLGRR784 pKa = 11.84TPDD787 pKa = 3.37SNFASGYY794 pKa = 9.29FDD796 pKa = 4.8IFISDD801 pKa = 4.5GNPLSDD807 pKa = 2.97ITADD811 pKa = 3.19NTFIDD816 pKa = 3.98GRR818 pKa = 11.84TQTAYY823 pKa = 10.84SGDD826 pKa = 4.11TNSGTIGAGGNPVGVSGSLLPIFEE850 pKa = 4.64LPEE853 pKa = 4.12IQVHH857 pKa = 6.33RR858 pKa = 11.84DD859 pKa = 3.0GGDD862 pKa = 3.42VLKK865 pKa = 11.1VQADD869 pKa = 3.65VVLRR873 pKa = 11.84NLSIYY878 pKa = 10.79ADD880 pKa = 3.64NTAGIQVGSGSAVIEE895 pKa = 4.13RR896 pKa = 11.84NLIGVNAQGTNSGNINYY913 pKa = 10.03GIEE916 pKa = 4.3NIGGSILIDD925 pKa = 3.41GNYY928 pKa = 9.66IATNTDD934 pKa = 2.2AGIFVNGGTTNIIQNNHH951 pKa = 4.4ITNNGNSACTDD962 pKa = 3.63NILVQGGSGLVITQNFIEE980 pKa = 4.47NAASAGIDD988 pKa = 3.28ASASSGNITISEE1000 pKa = 4.06NSITASGQDD1009 pKa = 3.42GGNCSGNIEE1018 pKa = 3.98GMAIKK1023 pKa = 10.26IGGNNSVVTNNMIYY1037 pKa = 10.77ANGGAGIVVVGGTANRR1053 pKa = 11.84ISQNSIYY1060 pKa = 11.06ANGTISDD1067 pKa = 4.14ALGIDD1072 pKa = 4.48LDD1074 pKa = 4.2ASSGAGDD1081 pKa = 4.12GVTLNDD1087 pKa = 4.52NGDD1090 pKa = 3.74GDD1092 pKa = 4.15TGPNDD1097 pKa = 3.7LQNFPIISAAYY1108 pKa = 8.58ISGSNLVVKK1117 pKa = 10.34GWSRR1121 pKa = 11.84PGATIEE1127 pKa = 5.38FFFTDD1132 pKa = 3.6INEE1135 pKa = 4.34GTATTGDD1142 pKa = 3.83NQLGMAVDD1150 pKa = 3.8YY1151 pKa = 11.67GEE1153 pKa = 4.39GQTFIGAAVEE1163 pKa = 4.33GSGADD1168 pKa = 3.46ADD1170 pKa = 4.29NTSSSYY1176 pKa = 11.66SDD1178 pKa = 3.31VDD1180 pKa = 3.85GNTDD1184 pKa = 2.99NTNRR1188 pKa = 11.84FHH1190 pKa = 7.56FNLPVPSGATLGEE1203 pKa = 4.73MITATATLGNSTSEE1217 pKa = 4.18FAPMSILRR1225 pKa = 11.84AATVITNRR1233 pKa = 11.84RR1234 pKa = 11.84ITYY1237 pKa = 9.66RR1238 pKa = 11.84VSKK1241 pKa = 10.76NN1242 pKa = 3.0

Molecular weight:
128.63 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0D5YRP8|A0A0D5YRP8_9FLAO Uncharacterized protein OS=Muricauda lutaonensis OX=516051 GN=VC82_1328 PE=4 SV=1
MM1 pKa = 7.46NEE3 pKa = 3.5VSLLDD8 pKa = 3.32WFLAFTAIFLLGISKK23 pKa = 10.51SGLKK27 pKa = 10.23GLSIFNVTLLALAFGARR44 pKa = 11.84EE45 pKa = 4.05STGILIPLLLVGDD58 pKa = 4.35VFAVLYY64 pKa = 9.15YY65 pKa = 10.44NRR67 pKa = 11.84HH68 pKa = 4.39TQWRR72 pKa = 11.84YY73 pKa = 9.19ILRR76 pKa = 11.84FLPWMISGILIGVFLGKK93 pKa = 9.89NLPEE97 pKa = 4.12TQFKK101 pKa = 10.11IGMVIVIFISLGVLTYY117 pKa = 9.61WDD119 pKa = 3.4RR120 pKa = 11.84RR121 pKa = 11.84KK122 pKa = 10.3SKK124 pKa = 10.37KK125 pKa = 10.44VPTHH129 pKa = 4.16WTFAGGVGILAGICTMIGNLAGAFTNIFFLAMRR162 pKa = 11.84LPKK165 pKa = 10.57NEE167 pKa = 4.14FVGTAAWLFLITNLFKK183 pKa = 11.08LPFHH187 pKa = 5.87IWVWKK192 pKa = 8.77TITPEE197 pKa = 3.93TVGINLKK204 pKa = 10.0LLPAILVGLAIGVRR218 pKa = 11.84IVRR221 pKa = 11.84RR222 pKa = 11.84INEE225 pKa = 3.62KK226 pKa = 8.96QYY228 pKa = 10.98RR229 pKa = 11.84RR230 pKa = 11.84FILIVTAIGAVVILLRR246 pKa = 4.58

Molecular weight:
27.62 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2882

0

2882

971754

50

4585

337.2

37.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.973 ± 0.047

0.746 ± 0.017

5.839 ± 0.055

6.859 ± 0.043

5.143 ± 0.037

6.989 ± 0.053

1.954 ± 0.03

6.91 ± 0.041

7.198 ± 0.079

9.491 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.028

5.332 ± 0.049

3.748 ± 0.028

3.475 ± 0.026

4.09 ± 0.038

5.798 ± 0.036

5.508 ± 0.066

6.663 ± 0.034

1.125 ± 0.019

3.832 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski