Amino acid dipepetide frequency for Escherichia phage Halfdan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.554AlaAla: 14.554 ± 2.32
0.951AlaCys: 0.951 ± 0.199
4.9AlaAsp: 4.9 ± 0.677
7.679AlaGlu: 7.679 ± 0.76
3.876AlaPhe: 3.876 ± 0.549
6.655AlaGly: 6.655 ± 0.722
1.316AlaHis: 1.316 ± 0.231
6.655AlaIle: 6.655 ± 0.894
7.241AlaLys: 7.241 ± 1.14
8.557AlaLeu: 8.557 ± 1.428
2.925AlaMet: 2.925 ± 0.484
5.339AlaAsn: 5.339 ± 0.696
4.242AlaPro: 4.242 ± 0.496
4.681AlaGln: 4.681 ± 0.832
4.461AlaArg: 4.461 ± 0.435
6.217AlaSer: 6.217 ± 0.884
7.314AlaThr: 7.314 ± 1.057
7.533AlaVal: 7.533 ± 0.891
1.243AlaTrp: 1.243 ± 0.301
2.925AlaTyr: 2.925 ± 0.415
0.0AlaXaa: 0.0 ± 0.0
Cys
1.316CysAla: 1.316 ± 0.315
0.146CysCys: 0.146 ± 0.115
1.17CysAsp: 1.17 ± 0.302
0.951CysGlu: 0.951 ± 0.26
0.146CysPhe: 0.146 ± 0.089
0.878CysGly: 0.878 ± 0.348
0.585CysHis: 0.585 ± 0.267
0.585CysIle: 0.585 ± 0.21
0.439CysLys: 0.439 ± 0.166
0.878CysLeu: 0.878 ± 0.278
0.0CysMet: 0.0 ± 0.0
0.439CysAsn: 0.439 ± 0.2
0.805CysPro: 0.805 ± 0.243
0.439CysGln: 0.439 ± 0.15
0.658CysArg: 0.658 ± 0.243
0.293CysSer: 0.293 ± 0.139
0.366CysThr: 0.366 ± 0.159
0.366CysVal: 0.366 ± 0.15
0.293CysTrp: 0.293 ± 0.159
0.366CysTyr: 0.366 ± 0.181
0.0CysXaa: 0.0 ± 0.0
Asp
5.558AspAla: 5.558 ± 0.632
0.658AspCys: 0.658 ± 0.211
3.072AspAsp: 3.072 ± 0.705
3.657AspGlu: 3.657 ± 0.469
2.194AspPhe: 2.194 ± 0.359
6.582AspGly: 6.582 ± 0.65
0.585AspHis: 0.585 ± 0.218
2.414AspIle: 2.414 ± 0.373
2.852AspLys: 2.852 ± 0.403
5.485AspLeu: 5.485 ± 0.862
1.17AspMet: 1.17 ± 0.297
2.999AspAsn: 2.999 ± 0.369
2.633AspPro: 2.633 ± 0.383
1.682AspGln: 1.682 ± 0.369
3.072AspArg: 3.072 ± 0.504
3.291AspSer: 3.291 ± 0.444
3.291AspThr: 3.291 ± 0.495
3.437AspVal: 3.437 ± 0.486
1.39AspTrp: 1.39 ± 0.3
2.267AspTyr: 2.267 ± 0.456
0.0AspXaa: 0.0 ± 0.0
Glu
6.655GluAla: 6.655 ± 0.789
1.17GluCys: 1.17 ± 0.326
2.194GluAsp: 2.194 ± 0.367
2.999GluGlu: 2.999 ± 0.571
2.267GluPhe: 2.267 ± 0.424
2.999GluGly: 2.999 ± 0.419
0.951GluHis: 0.951 ± 0.305
4.023GluIle: 4.023 ± 0.526
3.364GluLys: 3.364 ± 0.483
5.412GluLeu: 5.412 ± 0.674
2.121GluMet: 2.121 ± 0.376
1.828GluAsn: 1.828 ± 0.413
1.828GluPro: 1.828 ± 0.319
3.072GluGln: 3.072 ± 0.511
2.414GluArg: 2.414 ± 0.432
2.999GluSer: 2.999 ± 0.506
3.364GluThr: 3.364 ± 0.556
3.364GluVal: 3.364 ± 0.513
1.243GluTrp: 1.243 ± 0.279
2.999GluTyr: 2.999 ± 0.593
0.0GluXaa: 0.0 ± 0.0
Phe
3.437PheAla: 3.437 ± 0.746
0.512PheCys: 0.512 ± 0.249
4.242PheAsp: 4.242 ± 0.463
1.828PheGlu: 1.828 ± 0.464
1.39PhePhe: 1.39 ± 0.407
3.73PheGly: 3.73 ± 0.477
0.219PheHis: 0.219 ± 0.136
1.682PheIle: 1.682 ± 0.353
1.975PheLys: 1.975 ± 0.321
1.755PheLeu: 1.755 ± 0.494
1.097PheMet: 1.097 ± 0.222
1.902PheAsn: 1.902 ± 0.363
1.755PhePro: 1.755 ± 0.363
0.878PheGln: 0.878 ± 0.242
1.682PheArg: 1.682 ± 0.37
1.536PheSer: 1.536 ± 0.347
2.048PheThr: 2.048 ± 0.35
3.73PheVal: 3.73 ± 0.46
0.512PheTrp: 0.512 ± 0.28
1.39PheTyr: 1.39 ± 0.365
0.0PheXaa: 0.0 ± 0.0
Gly
7.826GlyAla: 7.826 ± 0.93
0.951GlyCys: 0.951 ± 0.236
5.046GlyAsp: 5.046 ± 0.506
4.534GlyGlu: 4.534 ± 0.745
3.803GlyPhe: 3.803 ± 0.565
6.802GlyGly: 6.802 ± 1.319
1.243GlyHis: 1.243 ± 0.315
4.534GlyIle: 4.534 ± 0.502
4.315GlyLys: 4.315 ± 0.452
6.143GlyLeu: 6.143 ± 0.497
1.682GlyMet: 1.682 ± 0.354
3.511GlyAsn: 3.511 ± 1.032
2.852GlyPro: 2.852 ± 0.435
4.388GlyGln: 4.388 ± 0.856
3.291GlyArg: 3.291 ± 0.413
3.803GlySer: 3.803 ± 0.52
4.681GlyThr: 4.681 ± 0.532
6.729GlyVal: 6.729 ± 0.811
2.194GlyTrp: 2.194 ± 0.486
3.218GlyTyr: 3.218 ± 0.367
0.0GlyXaa: 0.0 ± 0.0
His
1.024HisAla: 1.024 ± 0.227
0.073HisCys: 0.073 ± 0.071
0.366HisAsp: 0.366 ± 0.18
0.805HisGlu: 0.805 ± 0.228
0.805HisPhe: 0.805 ± 0.316
0.951HisGly: 0.951 ± 0.242
0.073HisHis: 0.073 ± 0.078
1.316HisIle: 1.316 ± 0.333
0.512HisLys: 0.512 ± 0.154
1.463HisLeu: 1.463 ± 0.292
0.146HisMet: 0.146 ± 0.088
0.585HisAsn: 0.585 ± 0.208
0.658HisPro: 0.658 ± 0.236
0.219HisGln: 0.219 ± 0.115
0.731HisArg: 0.731 ± 0.221
0.878HisSer: 0.878 ± 0.285
0.512HisThr: 0.512 ± 0.167
0.805HisVal: 0.805 ± 0.296
0.146HisTrp: 0.146 ± 0.091
0.585HisTyr: 0.585 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
7.533IleAla: 7.533 ± 0.789
0.731IleCys: 0.731 ± 0.26
4.023IleAsp: 4.023 ± 0.563
3.803IleGlu: 3.803 ± 0.454
1.097IlePhe: 1.097 ± 0.392
3.949IleGly: 3.949 ± 0.548
0.219IleHis: 0.219 ± 0.121
2.999IleIle: 2.999 ± 0.62
2.999IleLys: 2.999 ± 0.409
2.779IleLeu: 2.779 ± 0.492
0.658IleMet: 0.658 ± 0.199
2.633IleAsn: 2.633 ± 0.426
3.072IlePro: 3.072 ± 0.394
2.34IleGln: 2.34 ± 0.382
3.291IleArg: 3.291 ± 0.376
2.852IleSer: 2.852 ± 0.394
3.657IleThr: 3.657 ± 0.422
3.437IleVal: 3.437 ± 0.452
0.658IleTrp: 0.658 ± 0.241
2.414IleTyr: 2.414 ± 0.495
0.0IleXaa: 0.0 ± 0.0
Lys
7.899LysAla: 7.899 ± 0.999
0.512LysCys: 0.512 ± 0.215
3.364LysAsp: 3.364 ± 0.518
2.34LysGlu: 2.34 ± 0.545
1.609LysPhe: 1.609 ± 0.328
5.12LysGly: 5.12 ± 0.684
1.17LysHis: 1.17 ± 0.276
2.56LysIle: 2.56 ± 0.423
2.706LysLys: 2.706 ± 0.426
5.266LysLeu: 5.266 ± 0.568
1.316LysMet: 1.316 ± 0.274
1.975LysAsn: 1.975 ± 0.386
2.121LysPro: 2.121 ± 0.378
2.267LysGln: 2.267 ± 0.402
2.852LysArg: 2.852 ± 0.459
2.706LysSer: 2.706 ± 0.412
3.291LysThr: 3.291 ± 0.623
3.218LysVal: 3.218 ± 0.492
0.585LysTrp: 0.585 ± 0.218
1.975LysTyr: 1.975 ± 0.253
0.0LysXaa: 0.0 ± 0.0
Leu
6.948LeuAla: 6.948 ± 0.764
1.097LeuCys: 1.097 ± 0.28
4.096LeuAsp: 4.096 ± 0.469
3.657LeuGlu: 3.657 ± 0.516
3.437LeuPhe: 3.437 ± 0.498
5.412LeuGly: 5.412 ± 0.562
0.951LeuHis: 0.951 ± 0.236
3.949LeuIle: 3.949 ± 0.558
4.096LeuLys: 4.096 ± 0.505
5.339LeuLeu: 5.339 ± 0.655
1.755LeuMet: 1.755 ± 0.315
4.608LeuAsn: 4.608 ± 0.467
3.291LeuPro: 3.291 ± 0.46
3.584LeuGln: 3.584 ± 0.704
3.949LeuArg: 3.949 ± 0.566
5.924LeuSer: 5.924 ± 0.632
4.754LeuThr: 4.754 ± 0.635
4.534LeuVal: 4.534 ± 0.518
0.951LeuTrp: 0.951 ± 0.186
2.852LeuTyr: 2.852 ± 0.383
0.0LeuXaa: 0.0 ± 0.0
Met
2.56MetAla: 2.56 ± 0.378
0.073MetCys: 0.073 ± 0.066
0.731MetAsp: 0.731 ± 0.228
0.219MetGlu: 0.219 ± 0.121
0.878MetPhe: 0.878 ± 0.235
1.755MetGly: 1.755 ± 0.44
0.219MetHis: 0.219 ± 0.112
0.951MetIle: 0.951 ± 0.28
1.243MetLys: 1.243 ± 0.312
2.34MetLeu: 2.34 ± 0.407
0.878MetMet: 0.878 ± 0.29
1.243MetAsn: 1.243 ± 0.339
1.682MetPro: 1.682 ± 0.374
1.463MetGln: 1.463 ± 0.295
1.17MetArg: 1.17 ± 0.295
1.536MetSer: 1.536 ± 0.426
1.682MetThr: 1.682 ± 0.321
1.682MetVal: 1.682 ± 0.339
0.0MetTrp: 0.0 ± 0.0
0.439MetTyr: 0.439 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
4.534AsnAla: 4.534 ± 0.56
0.731AsnCys: 0.731 ± 0.281
2.852AsnAsp: 2.852 ± 0.47
3.145AsnGlu: 3.145 ± 0.683
1.097AsnPhe: 1.097 ± 0.367
6.07AsnGly: 6.07 ± 1.002
0.146AsnHis: 0.146 ± 0.094
2.633AsnIle: 2.633 ± 0.438
3.291AsnLys: 3.291 ± 0.506
2.633AsnLeu: 2.633 ± 0.359
0.951AsnMet: 0.951 ± 0.23
3.437AsnAsn: 3.437 ± 0.892
3.072AsnPro: 3.072 ± 0.443
2.56AsnGln: 2.56 ± 0.746
2.633AsnArg: 2.633 ± 0.462
2.706AsnSer: 2.706 ± 0.384
2.999AsnThr: 2.999 ± 0.467
3.949AsnVal: 3.949 ± 0.415
0.585AsnTrp: 0.585 ± 0.177
1.463AsnTyr: 1.463 ± 0.325
0.0AsnXaa: 0.0 ± 0.0
Pro
4.534ProAla: 4.534 ± 0.604
0.658ProCys: 0.658 ± 0.223
3.145ProAsp: 3.145 ± 0.555
2.925ProGlu: 2.925 ± 0.507
1.975ProPhe: 1.975 ± 0.402
3.657ProGly: 3.657 ± 0.577
0.805ProHis: 0.805 ± 0.215
2.34ProIle: 2.34 ± 0.506
2.487ProLys: 2.487 ± 0.487
2.706ProLeu: 2.706 ± 0.641
0.658ProMet: 0.658 ± 0.197
3.218ProAsn: 3.218 ± 0.616
2.487ProPro: 2.487 ± 0.506
1.609ProGln: 1.609 ± 0.425
2.487ProArg: 2.487 ± 0.422
2.34ProSer: 2.34 ± 0.465
4.169ProThr: 4.169 ± 0.63
3.072ProVal: 3.072 ± 0.399
0.731ProTrp: 0.731 ± 0.186
1.902ProTyr: 1.902 ± 0.432
0.0ProXaa: 0.0 ± 0.0
Gln
5.266GlnAla: 5.266 ± 1.022
0.293GlnCys: 0.293 ± 0.144
0.951GlnAsp: 0.951 ± 0.205
1.609GlnGlu: 1.609 ± 0.332
1.975GlnPhe: 1.975 ± 0.355
2.925GlnGly: 2.925 ± 0.684
0.585GlnHis: 0.585 ± 0.225
2.487GlnIle: 2.487 ± 0.414
1.902GlnLys: 1.902 ± 0.403
3.803GlnLeu: 3.803 ± 0.456
0.805GlnMet: 0.805 ± 0.202
2.925GlnAsn: 2.925 ± 0.711
2.194GlnPro: 2.194 ± 0.376
3.657GlnGln: 3.657 ± 0.811
2.194GlnArg: 2.194 ± 0.367
2.487GlnSer: 2.487 ± 0.373
1.902GlnThr: 1.902 ± 0.328
2.414GlnVal: 2.414 ± 0.477
0.512GlnTrp: 0.512 ± 0.213
1.243GlnTyr: 1.243 ± 0.303
0.0GlnXaa: 0.0 ± 0.0
Arg
3.876ArgAla: 3.876 ± 0.591
0.439ArgCys: 0.439 ± 0.184
3.291ArgAsp: 3.291 ± 0.476
2.048ArgGlu: 2.048 ± 0.323
1.536ArgPhe: 1.536 ± 0.387
2.34ArgGly: 2.34 ± 0.401
0.658ArgHis: 0.658 ± 0.216
3.949ArgIle: 3.949 ± 0.54
2.56ArgLys: 2.56 ± 0.454
4.315ArgLeu: 4.315 ± 0.535
2.048ArgMet: 2.048 ± 0.364
3.072ArgAsn: 3.072 ± 0.44
2.267ArgPro: 2.267 ± 0.407
1.536ArgGln: 1.536 ± 0.454
2.487ArgArg: 2.487 ± 0.349
2.487ArgSer: 2.487 ± 0.386
2.706ArgThr: 2.706 ± 0.499
2.852ArgVal: 2.852 ± 0.497
0.951ArgTrp: 0.951 ± 0.311
1.243ArgTyr: 1.243 ± 0.336
0.0ArgXaa: 0.0 ± 0.0
Ser
6.07SerAla: 6.07 ± 0.961
0.219SerCys: 0.219 ± 0.131
4.096SerAsp: 4.096 ± 0.551
4.023SerGlu: 4.023 ± 0.466
2.194SerPhe: 2.194 ± 0.369
4.534SerGly: 4.534 ± 0.581
0.878SerHis: 0.878 ± 0.254
2.34SerIle: 2.34 ± 0.517
2.925SerLys: 2.925 ± 0.457
4.315SerLeu: 4.315 ± 0.562
1.243SerMet: 1.243 ± 0.267
2.194SerAsn: 2.194 ± 0.459
2.925SerPro: 2.925 ± 0.448
1.828SerGln: 1.828 ± 0.369
2.34SerArg: 2.34 ± 0.475
2.633SerSer: 2.633 ± 0.449
3.437SerThr: 3.437 ± 0.539
4.096SerVal: 4.096 ± 0.459
0.731SerTrp: 0.731 ± 0.187
1.755SerTyr: 1.755 ± 0.327
0.0SerXaa: 0.0 ± 0.0
Thr
7.533ThrAla: 7.533 ± 1.555
0.512ThrCys: 0.512 ± 0.23
3.803ThrAsp: 3.803 ± 0.474
4.242ThrGlu: 4.242 ± 0.543
2.267ThrPhe: 2.267 ± 0.374
6.509ThrGly: 6.509 ± 0.577
0.512ThrHis: 0.512 ± 0.177
3.584ThrIle: 3.584 ± 0.623
3.437ThrLys: 3.437 ± 0.492
4.681ThrLeu: 4.681 ± 0.635
0.585ThrMet: 0.585 ± 0.192
3.291ThrAsn: 3.291 ± 0.519
3.73ThrPro: 3.73 ± 0.635
1.755ThrGln: 1.755 ± 0.245
2.267ThrArg: 2.267 ± 0.374
2.925ThrSer: 2.925 ± 0.49
3.657ThrThr: 3.657 ± 0.683
5.12ThrVal: 5.12 ± 0.693
0.512ThrTrp: 0.512 ± 0.183
1.828ThrTyr: 1.828 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
8.264ValAla: 8.264 ± 0.968
0.439ValCys: 0.439 ± 0.163
3.584ValAsp: 3.584 ± 0.432
3.949ValGlu: 3.949 ± 0.497
2.706ValPhe: 2.706 ± 0.502
6.363ValGly: 6.363 ± 0.64
1.024ValHis: 1.024 ± 0.26
3.803ValIle: 3.803 ± 0.433
4.096ValLys: 4.096 ± 0.502
4.388ValLeu: 4.388 ± 0.605
1.243ValMet: 1.243 ± 0.351
3.364ValAsn: 3.364 ± 0.569
3.437ValPro: 3.437 ± 0.607
2.414ValGln: 2.414 ± 0.407
2.121ValArg: 2.121 ± 0.427
3.803ValSer: 3.803 ± 0.658
5.412ValThr: 5.412 ± 0.83
4.9ValVal: 4.9 ± 0.883
1.463ValTrp: 1.463 ± 0.336
1.828ValTyr: 1.828 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
1.097TrpAla: 1.097 ± 0.318
0.366TrpCys: 0.366 ± 0.146
0.658TrpAsp: 0.658 ± 0.182
0.805TrpGlu: 0.805 ± 0.291
0.658TrpPhe: 0.658 ± 0.201
1.39TrpGly: 1.39 ± 0.419
0.219TrpHis: 0.219 ± 0.132
0.731TrpIle: 0.731 ± 0.21
0.731TrpLys: 0.731 ± 0.24
0.951TrpLeu: 0.951 ± 0.252
0.219TrpMet: 0.219 ± 0.111
0.805TrpAsn: 0.805 ± 0.312
1.024TrpPro: 1.024 ± 0.313
0.512TrpGln: 0.512 ± 0.172
0.878TrpArg: 0.878 ± 0.275
1.39TrpSer: 1.39 ± 0.308
1.024TrpThr: 1.024 ± 0.301
0.951TrpVal: 0.951 ± 0.24
0.366TrpTrp: 0.366 ± 0.162
0.731TrpTyr: 0.731 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.779TyrAla: 2.779 ± 0.452
0.585TyrCys: 0.585 ± 0.294
2.706TyrAsp: 2.706 ± 0.482
2.048TyrGlu: 2.048 ± 0.522
1.39TyrPhe: 1.39 ± 0.326
2.925TyrGly: 2.925 ± 0.482
0.293TyrHis: 0.293 ± 0.13
1.609TyrIle: 1.609 ± 0.37
1.682TyrLys: 1.682 ± 0.387
1.975TyrLeu: 1.975 ± 0.343
1.17TyrMet: 1.17 ± 0.308
2.121TyrAsn: 2.121 ± 0.357
1.828TyrPro: 1.828 ± 0.429
1.243TyrGln: 1.243 ± 0.338
1.755TyrArg: 1.755 ± 0.356
1.975TyrSer: 1.975 ± 0.358
2.487TyrThr: 2.487 ± 0.55
2.414TyrVal: 2.414 ± 0.456
0.439TyrTrp: 0.439 ± 0.128
1.39TyrTyr: 1.39 ± 0.471
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13674 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski