Physcomitrium patens (Spreading-leaved earth moss) (Physcomitrella patens)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Bryophyta; Bryophytina; Bryopsida; Funariidae; Funariales; Funariaceae; Physcomitrium

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47782 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K1ICN0|A0A2K1ICN0_PHYPA Uncharacterized protein OS=Physcomitrium patens OX=3218 GN=PHYPA_030524 PE=4 SV=1
MM1 pKa = 7.65APMADD6 pKa = 3.53FVDD9 pKa = 4.72DD10 pKa = 3.91KK11 pKa = 11.64GSTKK15 pKa = 10.26TYY17 pKa = 9.64LCHH20 pKa = 7.29DD21 pKa = 4.67LGYY24 pKa = 10.97APRR27 pKa = 11.84EE28 pKa = 3.97PDD30 pKa = 4.62DD31 pKa = 4.66VDD33 pKa = 5.64DD34 pKa = 4.82VDD36 pKa = 5.96DD37 pKa = 5.38DD38 pKa = 5.37GIYY41 pKa = 9.99TPPPLSPLPLPTNNIIEE58 pKa = 4.41DD59 pKa = 3.53VNANVTINTSANHH72 pKa = 4.99FTYY75 pKa = 10.91VSMSFKK81 pKa = 10.52IPSNPPDD88 pKa = 3.17

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K1K3B6|A0A2K1K3B6_PHYPA Isoform of A0A7I4ERJ9 Hexosyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA_012750 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.27WKK6 pKa = 9.53KK7 pKa = 9.88KK8 pKa = 8.64RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31365

16417

47782

19763506

12

5619

413.6

45.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.781 ± 0.014

1.796 ± 0.006

5.217 ± 0.008

6.601 ± 0.017

3.673 ± 0.008

6.893 ± 0.015

2.444 ± 0.005

4.522 ± 0.009

5.405 ± 0.012

9.586 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.419 ± 0.005

3.868 ± 0.008

5.094 ± 0.013

4.087 ± 0.01

5.994 ± 0.01

8.808 ± 0.012

5.156 ± 0.008

6.886 ± 0.009

1.274 ± 0.004

2.493 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski