Circovirus-like genome RW-B

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GIH8|C6GIH8_9VIRU Coat protein OS=Circovirus-like genome RW-B OX=642252 PE=4 SV=1
MM1 pKa = 7.81DD2 pKa = 4.28VDD4 pKa = 3.96TTPVSRR10 pKa = 11.84RR11 pKa = 11.84GRR13 pKa = 11.84PSSSSSSSSTGEE25 pKa = 3.66RR26 pKa = 11.84FTARR30 pKa = 11.84TFCVTVNNRR39 pKa = 11.84DD40 pKa = 3.42EE41 pKa = 5.54VEE43 pKa = 4.36QNWPNAVQEE52 pKa = 4.07PSEE55 pKa = 4.16EE56 pKa = 3.82FVEE59 pKa = 4.46RR60 pKa = 11.84VRR62 pKa = 11.84YY63 pKa = 8.49VVWQRR68 pKa = 11.84EE69 pKa = 4.24LAPEE73 pKa = 4.26TGRR76 pKa = 11.84VHH78 pKa = 5.23IQAYY82 pKa = 9.44VEE84 pKa = 4.51LYY86 pKa = 7.99MAAGIVMLKK95 pKa = 10.4RR96 pKa = 11.84LFNCPTMHH104 pKa = 5.61VEE106 pKa = 4.5KK107 pKa = 10.76RR108 pKa = 11.84RR109 pKa = 11.84GTQDD113 pKa = 2.9QARR116 pKa = 11.84NYY118 pKa = 9.97CMKK121 pKa = 10.21EE122 pKa = 3.95DD123 pKa = 3.97TRR125 pKa = 11.84DD126 pKa = 3.83GPDD129 pKa = 3.42GGPFEE134 pKa = 4.72YY135 pKa = 8.77GTYY138 pKa = 10.75SKK140 pKa = 9.96TPGNGQGKK148 pKa = 10.3RR149 pKa = 11.84NDD151 pKa = 3.3LTDD154 pKa = 3.44AVEE157 pKa = 4.0ALEE160 pKa = 4.42AGGVEE165 pKa = 4.33AVVSKK170 pKa = 11.01HH171 pKa = 5.41PNTYY175 pKa = 9.13VRR177 pKa = 11.84YY178 pKa = 9.29HH179 pKa = 6.61RR180 pKa = 11.84GIHH183 pKa = 6.31ALYY186 pKa = 9.48QAKK189 pKa = 10.52LEE191 pKa = 4.95AIASRR196 pKa = 11.84QQRR199 pKa = 11.84NVKK202 pKa = 8.46TAVFYY207 pKa = 11.11GEE209 pKa = 4.42PGTGKK214 pKa = 6.71THH216 pKa = 7.2CAFEE220 pKa = 4.16LARR223 pKa = 11.84ITGEE227 pKa = 3.84EE228 pKa = 4.22IYY230 pKa = 10.59ILNAPAGRR238 pKa = 11.84NQSVWFNGYY247 pKa = 8.23QNQKK251 pKa = 9.73ILVVDD256 pKa = 4.13EE257 pKa = 4.97MNGDD261 pKa = 3.97WIGWQLLLRR270 pKa = 11.84MTDD273 pKa = 4.21KK274 pKa = 11.41YY275 pKa = 11.03PLQCQTKK282 pKa = 10.07GGMVWAMWEE291 pKa = 4.04IVIFTSNAHH300 pKa = 5.64WEE302 pKa = 4.02DD303 pKa = 3.36WYY305 pKa = 10.38PYY307 pKa = 9.7YY308 pKa = 10.98AGGMDD313 pKa = 3.31KK314 pKa = 10.96AALKK318 pKa = 10.47RR319 pKa = 11.84RR320 pKa = 11.84IHH322 pKa = 6.1KK323 pKa = 9.25VVKK326 pKa = 10.17FYY328 pKa = 11.25GSEE331 pKa = 3.78ASGNFTKK338 pKa = 10.33KK339 pKa = 10.74YY340 pKa = 9.53YY341 pKa = 10.91DD342 pKa = 4.07PDD344 pKa = 3.64LPEE347 pKa = 5.21ANWTVATTDD356 pKa = 3.37VIATFLRR363 pKa = 11.84NGTVRR368 pKa = 11.84PHH370 pKa = 5.92TDD372 pKa = 2.99EE373 pKa = 4.26EE374 pKa = 4.71LEE376 pKa = 4.15EE377 pKa = 4.41VEE379 pKa = 4.33DD380 pKa = 4.2VVMNTQQEE388 pKa = 4.58SQGTQTTEE396 pKa = 3.79LVEE399 pKa = 5.35DD400 pKa = 4.42SDD402 pKa = 6.05DD403 pKa = 3.84EE404 pKa = 4.21MSEE407 pKa = 4.29TFF409 pKa = 4.24

Molecular weight: 46.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GIH8|C6GIH8_9VIRU Coat protein OS=Circovirus-like genome RW-B OX=642252 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TNPYY8 pKa = 9.39QRR10 pKa = 11.84ASLPPKK16 pKa = 9.38KK17 pKa = 9.86RR18 pKa = 11.84YY19 pKa = 9.42RR20 pKa = 11.84ADD22 pKa = 3.35QQPAIPRR29 pKa = 11.84LRR31 pKa = 11.84LPYY34 pKa = 10.26HH35 pKa = 6.73LGGPLYY41 pKa = 10.52VSRR44 pKa = 11.84PLPMARR50 pKa = 11.84RR51 pKa = 11.84GAPTGRR57 pKa = 11.84YY58 pKa = 9.15ASNPRR63 pKa = 11.84MGEE66 pKa = 4.05LKK68 pKa = 9.9TVDD71 pKa = 3.78YY72 pKa = 10.72QFSAAYY78 pKa = 8.95AVPYY82 pKa = 10.67VGDD85 pKa = 3.85SQPFPLLNCTNATGNIQCVNLVQQGTGVAQRR116 pKa = 11.84IGNKK120 pKa = 8.72ICMRR124 pKa = 11.84SLRR127 pKa = 11.84IRR129 pKa = 11.84LRR131 pKa = 11.84FVEE134 pKa = 5.53AGTDD138 pKa = 3.8DD139 pKa = 3.62NQAPYY144 pKa = 9.69FARR147 pKa = 11.84IMLIYY152 pKa = 10.39DD153 pKa = 4.04RR154 pKa = 11.84NPNSLYY160 pKa = 10.33IATNNILANALQNNTLGNGTLDD182 pKa = 3.16SSLNPTYY189 pKa = 10.58YY190 pKa = 10.78DD191 pKa = 3.07RR192 pKa = 11.84FVVLRR197 pKa = 11.84DD198 pKa = 3.44WYY200 pKa = 9.09QTIPTFQEE208 pKa = 3.91STSNVAPNWIIDD220 pKa = 3.73DD221 pKa = 4.11FVNLKK226 pKa = 10.23NLEE229 pKa = 4.02TTYY232 pKa = 11.28NGTANPMTINQVSVGALLLFVISDD256 pKa = 3.59QVAATSDD263 pKa = 3.48AVALGGNLRR272 pKa = 11.84LRR274 pKa = 11.84FHH276 pKa = 7.04DD277 pKa = 3.49QQ278 pKa = 2.85

Molecular weight: 31.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

687

278

409

343.5

38.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.715 ± 0.588

1.164 ± 0.055

5.24 ± 0.131

5.822 ± 2.806

3.202 ± 0.022

6.987 ± 0.558

1.601 ± 0.564

3.93 ± 0.708

3.493 ± 0.855

6.987 ± 1.975

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.296

6.55 ± 1.563

5.386 ± 1.158

5.24 ± 0.33

7.132 ± 0.5

5.095 ± 0.193

7.569 ± 0.47

7.569 ± 0.931

1.747 ± 0.658

4.949 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski