Amino acid dipepetide frequency for Clostridium saccharoperbutylacetonicum N1-4(HMT)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.245AlaAla: 4.245 ± 0.062	0.756AlaCys: 0.756 ± 0.022	2.981AlaAsp: 2.981 ± 0.046	3.727AlaGlu: 3.727 ± 0.049	2.663AlaPhe: 2.663 ± 0.047	3.927AlaGly: 3.927 ± 0.057	0.821AlaHis: 0.821 ± 0.023	5.68AlaIle: 5.68 ± 0.064	4.79AlaLys: 4.79 ± 0.052	5.73AlaLeu: 5.73 ± 0.056	1.654AlaMet: 1.654 ± 0.028	2.994AlaAsn: 2.994 ± 0.047	1.456AlaPro: 1.456 ± 0.036	1.607AlaGln: 1.607 ± 0.051	1.773AlaArg: 1.773 ± 0.03	3.62AlaSer: 3.62 ± 0.051	3.128AlaThr: 3.128 ± 0.099	3.971AlaVal: 3.971 ± 0.057	0.468AlaTrp: 0.468 ± 0.017	2.278AlaTyr: 2.278 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	0.636CysAla: 0.636 ± 0.02	0.19CysCys: 0.19 ± 0.012	0.708CysAsp: 0.708 ± 0.02	0.802CysGlu: 0.802 ± 0.024	0.545CysPhe: 0.545 ± 0.017	1.06CysGly: 1.06 ± 0.028	0.228CysHis: 0.228 ± 0.01	1.149CysIle: 1.149 ± 0.027	0.993CysLys: 0.993 ± 0.022	0.918CysLeu: 0.918 ± 0.027	0.309CysMet: 0.309 ± 0.013	0.753CysAsn: 0.753 ± 0.023	0.476CysPro: 0.476 ± 0.015	0.217CysGln: 0.217 ± 0.011	0.386CysArg: 0.386 ± 0.016	0.795CysSer: 0.795 ± 0.023	0.601CysThr: 0.601 ± 0.019	0.693CysVal: 0.693 ± 0.019	0.087CysTrp: 0.087 ± 0.007	0.504CysTyr: 0.504 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	2.847AspAla: 2.847 ± 0.041	0.647AspCys: 0.647 ± 0.021	2.924AspAsp: 2.924 ± 0.05	4.549AspGlu: 4.549 ± 0.058	2.762AspPhe: 2.762 ± 0.038	3.814AspGly: 3.814 ± 0.058	0.576AspHis: 0.576 ± 0.016	5.832AspIle: 5.832 ± 0.063	5.473AspLys: 5.473 ± 0.056	4.851AspLeu: 4.851 ± 0.058	1.432AspMet: 1.432 ± 0.028	3.704AspAsn: 3.704 ± 0.045	1.372AspPro: 1.372 ± 0.033	0.953AspGln: 0.953 ± 0.026	1.709AspArg: 1.709 ± 0.036	3.445AspSer: 3.445 ± 0.048	2.628AspThr: 2.628 ± 0.04	3.335AspVal: 3.335 ± 0.042	0.474AspTrp: 0.474 ± 0.017	2.739AspTyr: 2.739 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.428GluAla: 4.428 ± 0.066	0.765GluCys: 0.765 ± 0.023	4.049GluAsp: 4.049 ± 0.056	6.705GluGlu: 6.705 ± 0.095	3.077GluPhe: 3.077 ± 0.048	3.795GluGly: 3.795 ± 0.044	1.038GluHis: 1.038 ± 0.028	7.364GluIle: 7.364 ± 0.071	7.376GluLys: 7.376 ± 0.094	6.862GluLeu: 6.862 ± 0.068	1.983GluMet: 1.983 ± 0.036	5.515GluAsn: 5.515 ± 0.066	1.431GluPro: 1.431 ± 0.027	1.956GluGln: 1.956 ± 0.038	2.408GluArg: 2.408 ± 0.045	3.484GluSer: 3.484 ± 0.044	3.057GluThr: 3.057 ± 0.04	4.857GluVal: 4.857 ± 0.056	0.578GluTrp: 0.578 ± 0.022	3.17GluTyr: 3.17 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	2.366PheAla: 2.366 ± 0.039	0.541PheCys: 0.541 ± 0.018	2.733PheAsp: 2.733 ± 0.035	3.001PheGlu: 3.001 ± 0.046	2.022PhePhe: 2.022 ± 0.036	2.943PheGly: 2.943 ± 0.047	0.591PheHis: 0.591 ± 0.019	4.34PheIle: 4.34 ± 0.06	3.781PheLys: 3.781 ± 0.046	3.732PheLeu: 3.732 ± 0.055	1.149PheMet: 1.149 ± 0.027	3.053PheAsn: 3.053 ± 0.041	1.151PhePro: 1.151 ± 0.028	1.001PheGln: 1.001 ± 0.026	1.199PheArg: 1.199 ± 0.027	3.13PheSer: 3.13 ± 0.046	2.372PheThr: 2.372 ± 0.035	2.631PheVal: 2.631 ± 0.038	0.358PheTrp: 0.358 ± 0.013	1.875PheTyr: 1.875 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.218GlyAla: 4.218 ± 0.102	0.918GlyCys: 0.918 ± 0.026	3.249GlyAsp: 3.249 ± 0.044	4.221GlyGlu: 4.221 ± 0.05	3.005GlyPhe: 3.005 ± 0.039	4.24GlyGly: 4.24 ± 0.074	0.944GlyHis: 0.944 ± 0.026	6.638GlyIle: 6.638 ± 0.073	5.351GlyLys: 5.351 ± 0.055	5.156GlyLeu: 5.156 ± 0.065	1.778GlyMet: 1.778 ± 0.038	3.554GlyAsn: 3.554 ± 0.055	1.201GlyPro: 1.201 ± 0.032	1.602GlyGln: 1.602 ± 0.034	1.998GlyArg: 1.998 ± 0.034	3.661GlySer: 3.661 ± 0.05	3.697GlyThr: 3.697 ± 0.06	4.426GlyVal: 4.426 ± 0.063	0.728GlyTrp: 0.728 ± 0.03	3.044GlyTyr: 3.044 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	0.7HisAla: 0.7 ± 0.02	0.224HisCys: 0.224 ± 0.011	0.759HisAsp: 0.759 ± 0.019	0.939HisGlu: 0.939 ± 0.022	0.66HisPhe: 0.66 ± 0.021	1.01HisGly: 1.01 ± 0.024	0.308HisHis: 0.308 ± 0.022	1.308HisIle: 1.308 ± 0.027	1.088HisLys: 1.088 ± 0.025	1.169HisLeu: 1.169 ± 0.027	0.352HisMet: 0.352 ± 0.013	0.779HisAsn: 0.779 ± 0.019	0.549HisPro: 0.549 ± 0.016	0.33HisGln: 0.33 ± 0.012	0.483HisArg: 0.483 ± 0.016	0.869HisSer: 0.869 ± 0.019	0.641HisThr: 0.641 ± 0.018	0.705HisVal: 0.705 ± 0.02	0.128HisTrp: 0.128 ± 0.009	0.621HisTyr: 0.621 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.765IleAla: 5.765 ± 0.064	1.214IleCys: 1.214 ± 0.028	5.734IleAsp: 5.734 ± 0.066	7.072IleGlu: 7.072 ± 0.078	4.037IlePhe: 4.037 ± 0.058	6.077IleGly: 6.077 ± 0.066	1.264IleHis: 1.264 ± 0.025	9.206IleIle: 9.206 ± 0.117	8.738IleLys: 8.738 ± 0.082	8.668IleLeu: 8.668 ± 0.092	2.259IleMet: 2.259 ± 0.036	6.767IleAsn: 6.767 ± 0.064	3.252IlePro: 3.252 ± 0.054	2.343IleGln: 2.343 ± 0.037	2.868IleArg: 2.868 ± 0.042	7.184IleSer: 7.184 ± 0.069	5.13IleThr: 5.13 ± 0.057	5.766IleVal: 5.766 ± 0.067	0.625IleTrp: 0.625 ± 0.018	3.647IleTyr: 3.647 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	5.205LysAla: 5.205 ± 0.054	0.911LysCys: 0.911 ± 0.025	5.637LysAsp: 5.637 ± 0.062	8.401LysGlu: 8.401 ± 0.093	3.362LysPhe: 3.362 ± 0.049	4.688LysGly: 4.688 ± 0.054	1.153LysHis: 1.153 ± 0.023	8.295LysIle: 8.295 ± 0.076	7.596LysLys: 7.596 ± 0.07	7.74LysLeu: 7.74 ± 0.067	2.311LysMet: 2.311 ± 0.037	6.292LysAsn: 6.292 ± 0.067	2.034LysPro: 2.034 ± 0.032	2.313LysGln: 2.313 ± 0.04	2.803LysArg: 2.803 ± 0.042	4.967LysSer: 4.967 ± 0.057	4.066LysThr: 4.066 ± 0.046	5.789LysVal: 5.789 ± 0.061	0.737LysTrp: 0.737 ± 0.024	4.14LysTyr: 4.14 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	5.171LeuAla: 5.171 ± 0.065	1.111LeuCys: 1.111 ± 0.022	5.038LeuAsp: 5.038 ± 0.06	6.431LeuGlu: 6.431 ± 0.069	3.736LeuPhe: 3.736 ± 0.052	5.735LeuGly: 5.735 ± 0.066	1.086LeuHis: 1.086 ± 0.026	8.238LeuIle: 8.238 ± 0.103	8.306LeuLys: 8.306 ± 0.081	7.653LeuLeu: 7.653 ± 0.089	2.283LeuMet: 2.283 ± 0.036	5.979LeuAsn: 5.979 ± 0.057	2.683LeuPro: 2.683 ± 0.041	2.247LeuGln: 2.247 ± 0.037	2.867LeuArg: 2.867 ± 0.041	6.391LeuSer: 6.391 ± 0.051	4.48LeuThr: 4.48 ± 0.058	5.094LeuVal: 5.094 ± 0.056	0.657LeuTrp: 0.657 ± 0.021	3.297LeuTyr: 3.297 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	1.68MetAla: 1.68 ± 0.034	0.278MetCys: 0.278 ± 0.012	1.459MetAsp: 1.459 ± 0.031	1.827MetGlu: 1.827 ± 0.035	1.016MetPhe: 1.016 ± 0.023	1.629MetGly: 1.629 ± 0.033	0.357MetHis: 0.357 ± 0.013	2.222MetIle: 2.222 ± 0.037	2.583MetLys: 2.583 ± 0.039	2.343MetLeu: 2.343 ± 0.039	0.647MetMet: 0.647 ± 0.021	1.76MetAsn: 1.76 ± 0.034	0.954MetPro: 0.954 ± 0.021	0.733MetGln: 0.733 ± 0.021	0.791MetArg: 0.791 ± 0.02	1.781MetSer: 1.781 ± 0.031	1.226MetThr: 1.226 ± 0.023	1.481MetVal: 1.481 ± 0.033	0.165MetTrp: 0.165 ± 0.01	0.852MetTyr: 0.852 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.256AsnAla: 3.256 ± 0.038	0.774AsnCys: 0.774 ± 0.022	3.46AsnAsp: 3.46 ± 0.045	4.738AsnGlu: 4.738 ± 0.06	2.739AsnPhe: 2.739 ± 0.039	4.136AsnGly: 4.136 ± 0.064	0.828AsnHis: 0.828 ± 0.022	7.069AsnIle: 7.069 ± 0.075	6.057AsnLys: 6.057 ± 0.064	5.806AsnLeu: 5.806 ± 0.055	1.701AsnMet: 1.701 ± 0.034	4.941AsnAsn: 4.941 ± 0.059	2.105AsnPro: 2.105 ± 0.04	1.62AsnGln: 1.62 ± 0.032	1.914AsnArg: 1.914 ± 0.033	4.605AsnSer: 4.605 ± 0.057	3.223AsnThr: 3.223 ± 0.039	3.873AsnVal: 3.873 ± 0.049	0.53AsnTrp: 0.53 ± 0.017	3.093AsnTyr: 3.093 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	1.489ProAla: 1.489 ± 0.034	0.322ProCys: 0.322 ± 0.014	1.467ProAsp: 1.467 ± 0.029	2.11ProGlu: 2.11 ± 0.036	1.366ProPhe: 1.366 ± 0.033	1.638ProGly: 1.638 ± 0.037	0.462ProHis: 0.462 ± 0.014	2.713ProIle: 2.713 ± 0.046	2.212ProLys: 2.212 ± 0.037	2.469ProLeu: 2.469 ± 0.035	0.713ProMet: 0.713 ± 0.019	1.71ProAsn: 1.71 ± 0.034	0.568ProPro: 0.568 ± 0.02	0.784ProGln: 0.784 ± 0.028	0.764ProArg: 0.764 ± 0.022	1.72ProSer: 1.72 ± 0.028	1.485ProThr: 1.485 ± 0.032	1.898ProVal: 1.898 ± 0.039	0.273ProTrp: 0.273 ± 0.013	1.261ProTyr: 1.261 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	1.557GlnAla: 1.557 ± 0.033	0.282GlnCys: 0.282 ± 0.012	1.325GlnAsp: 1.325 ± 0.027	1.794GlnGlu: 1.794 ± 0.038	1.018GlnPhe: 1.018 ± 0.026	1.739GlnGly: 1.739 ± 0.111	0.326GlnHis: 0.326 ± 0.014	2.312GlnIle: 2.312 ± 0.036	2.236GlnLys: 2.236 ± 0.034	2.202GlnLeu: 2.202 ± 0.033	0.699GlnMet: 0.699 ± 0.018	1.727GlnAsn: 1.727 ± 0.032	0.535GlnPro: 0.535 ± 0.017	0.693GlnGln: 0.693 ± 0.022	0.844GlnArg: 0.844 ± 0.022	1.426GlnSer: 1.426 ± 0.033	1.224GlnThr: 1.224 ± 0.028	1.585GlnVal: 1.585 ± 0.033	0.242GlnTrp: 0.242 ± 0.011	1.147GlnTyr: 1.147 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	1.784ArgAla: 1.784 ± 0.033	0.394ArgCys: 0.394 ± 0.015	1.656ArgAsp: 1.656 ± 0.032	2.536ArgGlu: 2.536 ± 0.045	1.358ArgPhe: 1.358 ± 0.026	1.735ArgGly: 1.735 ± 0.03	0.434ArgHis: 0.434 ± 0.017	3.105ArgIle: 3.105 ± 0.052	2.872ArgLys: 2.872 ± 0.036	2.683ArgLeu: 2.683 ± 0.05	0.833ArgMet: 0.833 ± 0.025	2.049ArgAsn: 2.049 ± 0.037	0.785ArgPro: 0.785 ± 0.023	0.861ArgGln: 0.861 ± 0.029	1.225ArgArg: 1.225 ± 0.026	1.545ArgSer: 1.545 ± 0.033	1.53ArgThr: 1.53 ± 0.03	1.932ArgVal: 1.932 ± 0.035	0.273ArgTrp: 0.273 ± 0.013	1.393ArgTyr: 1.393 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	3.457SerAla: 3.457 ± 0.047	0.655SerCys: 0.655 ± 0.02	3.54SerAsp: 3.54 ± 0.055	4.349SerGlu: 4.349 ± 0.064	3.002SerPhe: 3.002 ± 0.042	4.391SerGly: 4.391 ± 0.055	0.864SerHis: 0.864 ± 0.023	6.53SerIle: 6.53 ± 0.063	5.692SerLys: 5.692 ± 0.059	5.722SerLeu: 5.722 ± 0.054	1.666SerMet: 1.666 ± 0.029	4.283SerAsn: 4.283 ± 0.058	1.635SerPro: 1.635 ± 0.032	1.683SerGln: 1.683 ± 0.042	1.931SerArg: 1.931 ± 0.035	4.437SerSer: 4.437 ± 0.062	3.392SerThr: 3.392 ± 0.047	3.771SerVal: 3.771 ± 0.043	0.523SerTrp: 0.523 ± 0.021	2.642SerTyr: 2.642 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	3.215ThrAla: 3.215 ± 0.053	0.555ThrCys: 0.555 ± 0.018	2.625ThrAsp: 2.625 ± 0.044	3.076ThrGlu: 3.076 ± 0.043	2.336ThrPhe: 2.336 ± 0.041	3.907ThrGly: 3.907 ± 0.155	0.746ThrHis: 0.746 ± 0.021	4.9ThrIle: 4.9 ± 0.057	3.81ThrLys: 3.81 ± 0.039	4.694ThrLeu: 4.694 ± 0.055	1.141ThrMet: 1.141 ± 0.025	3.093ThrAsn: 3.093 ± 0.047	1.822ThrPro: 1.822 ± 0.032	1.314ThrGln: 1.314 ± 0.029	1.434ThrArg: 1.434 ± 0.031	3.362ThrSer: 3.362 ± 0.046	2.97ThrThr: 2.97 ± 0.051	3.382ThrVal: 3.382 ± 0.052	0.44ThrTrp: 0.44 ± 0.018	2.088ThrTyr: 2.088 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	3.742ValAla: 3.742 ± 0.055	0.831ValCys: 0.831 ± 0.018	3.721ValAsp: 3.721 ± 0.044	4.177ValGlu: 4.177 ± 0.054	2.774ValPhe: 2.774 ± 0.05	3.953ValGly: 3.953 ± 0.052	0.821ValHis: 0.821 ± 0.024	5.91ValIle: 5.91 ± 0.07	5.179ValLys: 5.179 ± 0.054	5.504ValLeu: 5.504 ± 0.065	1.597ValMet: 1.597 ± 0.03	3.922ValAsn: 3.922 ± 0.047	1.902ValPro: 1.902 ± 0.033	1.496ValGln: 1.496 ± 0.033	1.877ValArg: 1.877 ± 0.035	4.299ValSer: 4.299 ± 0.056	3.484ValThr: 3.484 ± 0.073	4.24ValVal: 4.24 ± 0.056	0.451ValTrp: 0.451 ± 0.018	2.383ValTyr: 2.383 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	0.441TrpAla: 0.441 ± 0.016	0.107TrpCys: 0.107 ± 0.008	0.453TrpAsp: 0.453 ± 0.017	0.476TrpGlu: 0.476 ± 0.017	0.402TrpPhe: 0.402 ± 0.018	0.565TrpGly: 0.565 ± 0.021	0.149TrpHis: 0.149 ± 0.008	0.787TrpIle: 0.787 ± 0.021	0.627TrpLys: 0.627 ± 0.02	0.679TrpLeu: 0.679 ± 0.02	0.232TrpMet: 0.232 ± 0.012	0.574TrpAsn: 0.574 ± 0.021	0.206TrpPro: 0.206 ± 0.011	0.266TrpGln: 0.266 ± 0.013	0.282TrpArg: 0.282 ± 0.013	0.504TrpSer: 0.504 ± 0.018	0.417TrpThr: 0.417 ± 0.018	0.439TrpVal: 0.439 ± 0.016	0.131TrpTrp: 0.131 ± 0.009	0.476TrpTyr: 0.476 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.068TyrAla: 2.068 ± 0.035	0.577TyrCys: 0.577 ± 0.021	2.545TyrAsp: 2.545 ± 0.049	2.966TyrGlu: 2.966 ± 0.046	2.091TyrPhe: 2.091 ± 0.036	2.646TyrGly: 2.646 ± 0.04	0.626TyrHis: 0.626 ± 0.019	4.019TyrIle: 4.019 ± 0.05	3.68TyrLys: 3.68 ± 0.052	3.901TyrLeu: 3.901 ± 0.056	1.046TyrMet: 1.046 ± 0.026	3.017TyrAsn: 3.017 ± 0.052	1.309TyrPro: 1.309 ± 0.029	0.895TyrGln: 0.895 ± 0.024	1.427TyrArg: 1.427 ± 0.03	2.937TyrSer: 2.937 ± 0.047	2.162TyrThr: 2.162 ± 0.038	2.396TyrVal: 2.396 ± 0.032	0.344TyrTrp: 0.344 ± 0.014	2.193TyrTyr: 2.193 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5810 proteins (1832897 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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